Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06915 and MMJJ_RS06920 are separated by 183 nucleotides MMJJ_RS06920 and MMJJ_RS06925 are separated by 39 nucleotides MMJJ_RS06925 and MMJJ_RS06930 are separated by 19 nucleotides MMJJ_RS06930 and MMJJ_RS06935 are separated by 10 nucleotides
MMJJ_RS06915: MMJJ_RS06915 - helix-turn-helix domain-containing protein, at 1,269,720 to 1,270,358
_RS06915
MMJJ_RS06920: MMJJ_RS06920 - ZPR1 zinc finger domain-containing protein, at 1,270,542 to 1,271,114
_RS06920
MMJJ_RS06925: MMJJ_RS06925 - GMP synthase subunit A, at 1,271,154 to 1,271,723
_RS06925
MMJJ_RS06930: MMJJ_RS06930 - type II methionyl aminopeptidase, at 1,271,743 to 1,272,630
_RS06930
MMJJ_RS06935: MMJJ_RS06935 - GTP-binding protein, at 1,272,641 to 1,273,750
_RS06935
Position (kb) 1271
1272 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1270.157 kb on - strand, within MMJJ_RS06915 at 1270.158 kb on + strand, within MMJJ_RS06915 at 1270.302 kb on - strand at 1270.326 kb on + strand at 1270.345 kb on + strand at 1270.430 kb on - strand at 1270.431 kb on + strand at 1270.434 kb on - strand at 1270.434 kb on - strand at 1270.434 kb on - strand at 1270.434 kb on - strand at 1270.435 kb on + strand at 1270.437 kb on + strand at 1270.472 kb on - strand at 1270.472 kb on - strand at 1270.472 kb on - strand at 1270.472 kb on - strand at 1270.473 kb on + strand at 1270.473 kb on + strand at 1270.494 kb on + strand at 1270.498 kb on - strand at 1270.499 kb on + strand at 1270.499 kb on + strand at 1270.499 kb on + strand at 1270.509 kb on + strand at 1270.561 kb on - strand at 1270.728 kb on + strand at 1270.772 kb on + strand, within MMJJ_RS06920 at 1270.846 kb on + strand, within MMJJ_RS06920 at 1271.083 kb on - strand at 1271.093 kb on - strand at 1271.094 kb on + strand at 1271.096 kb on - strand at 1271.096 kb on - strand at 1271.098 kb on - strand at 1271.099 kb on + strand at 1271.099 kb on + strand at 1271.103 kb on + strand at 1271.103 kb on + strand at 1271.113 kb on + strand at 1271.113 kb on + strand at 1271.142 kb on - strand at 1271.142 kb on - strand at 1271.146 kb on - strand at 1271.147 kb on + strand at 1271.549 kb on - strand, within MMJJ_RS06925 at 1271.641 kb on + strand at 1271.688 kb on - strand at 1271.957 kb on + strand, within MMJJ_RS06930 at 1272.013 kb on + strand, within MMJJ_RS06930 at 1272.013 kb on + strand at 1272.160 kb on - strand, within MMJJ_RS06930 at 1272.161 kb on + strand, within MMJJ_RS06930 at 1272.277 kb on - strand, within MMJJ_RS06930 at 1272.328 kb on - strand, within MMJJ_RS06930 at 1272.554 kb on + strand at 1272.610 kb on + strand at 1272.671 kb on - strand at 1272.671 kb on - strand at 1272.672 kb on + strand at 1272.674 kb on + strand at 1272.674 kb on + strand at 1272.686 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 1,270,157 - MMJJ_RS06915 0.68 -0.2 1,270,158 + MMJJ_RS06915 0.69 -0.6 1,270,302 - +0.7 1,270,326 + -0.2 1,270,345 + -1.1 1,270,430 - -2.1 1,270,431 + -0.5 1,270,434 - +0.4 1,270,434 - +0.8 1,270,434 - +0.2 1,270,434 - -0.3 1,270,435 + +0.4 1,270,437 + -0.3 1,270,472 - -0.5 1,270,472 - +0.1 1,270,472 - +0.6 1,270,472 - -0.6 1,270,473 + +0.0 1,270,473 + +0.9 1,270,494 + +1.1 1,270,498 - -3.4 1,270,499 + +0.1 1,270,499 + -0.5 1,270,499 + -1.1 1,270,509 + -2.1 1,270,561 - -0.5 1,270,728 + +0.5 1,270,772 + MMJJ_RS06920 0.40 -0.5 1,270,846 + MMJJ_RS06920 0.53 -0.5 1,271,083 - -1.6 1,271,093 - +0.6 1,271,094 + -0.1 1,271,096 - -0.4 1,271,096 - -0.8 1,271,098 - -3.1 1,271,099 + -4.0 1,271,099 + -3.6 1,271,103 + +2.1 1,271,103 + -0.1 1,271,113 + +0.4 1,271,113 + +0.3 1,271,142 - +0.9 1,271,142 - +0.2 1,271,146 - -0.5 1,271,147 + -0.4 1,271,549 - MMJJ_RS06925 0.69 -0.7 1,271,641 + -0.5 1,271,688 - -1.6 1,271,957 + MMJJ_RS06930 0.24 -1.3 1,272,013 + MMJJ_RS06930 0.30 -2.5 1,272,013 + -1.3 1,272,160 - MMJJ_RS06930 0.47 -2.3 1,272,161 + MMJJ_RS06930 0.47 -1.5 1,272,277 - MMJJ_RS06930 0.60 +0.5 1,272,328 - MMJJ_RS06930 0.66 -2.4 1,272,554 + -1.3 1,272,610 + +0.5 1,272,671 - -3.2 1,272,671 - -3.6 1,272,672 + -1.6 1,272,674 + -3.1 1,272,674 + -3.6 1,272,686 - -2.3
Or see this region's nucleotide sequence