Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05005 and MMJJ_RS05010 are separated by 321 nucleotides MMJJ_RS05010 and MMJJ_RS05015 are separated by 32 nucleotides MMJJ_RS05015 and MMJJ_RS05020 are separated by 20 nucleotides
MMJJ_RS05005: MMJJ_RS05005 - hypothetical protein, at 929,531 to 930,271
_RS05005
MMJJ_RS05010: MMJJ_RS05010 - Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain, at 930,593 to 931,672
_RS05010
MMJJ_RS05015: MMJJ_RS05015 - hypothetical protein, at 931,705 to 932,475
_RS05015
MMJJ_RS05020: MMJJ_RS05020 - glutamate synthase-related protein, at 932,496 to 934,028
_RS05020
Position (kb) 930
931
932 Strain fitness (log2 ratio) -1
0
1
2
3 at 929.593 kb on + strand at 929.594 kb on - strand at 929.594 kb on - strand at 929.594 kb on - strand at 929.594 kb on - strand at 929.594 kb on - strand at 929.594 kb on - strand at 929.595 kb on + strand at 929.595 kb on + strand at 929.597 kb on - strand at 929.598 kb on + strand at 929.598 kb on + strand at 929.614 kb on + strand, within MMJJ_RS05005 at 929.640 kb on - strand, within MMJJ_RS05005 at 929.640 kb on - strand, within MMJJ_RS05005 at 929.642 kb on - strand, within MMJJ_RS05005 at 929.665 kb on - strand, within MMJJ_RS05005 at 929.674 kb on + strand, within MMJJ_RS05005 at 929.683 kb on + strand, within MMJJ_RS05005 at 929.753 kb on + strand, within MMJJ_RS05005 at 929.753 kb on + strand, within MMJJ_RS05005 at 929.796 kb on - strand, within MMJJ_RS05005 at 929.801 kb on - strand, within MMJJ_RS05005 at 929.812 kb on - strand, within MMJJ_RS05005 at 929.827 kb on + strand, within MMJJ_RS05005 at 929.850 kb on - strand, within MMJJ_RS05005 at 929.854 kb on + strand, within MMJJ_RS05005 at 929.854 kb on + strand, within MMJJ_RS05005 at 929.860 kb on - strand, within MMJJ_RS05005 at 929.894 kb on + strand, within MMJJ_RS05005 at 929.986 kb on + strand, within MMJJ_RS05005 at 929.986 kb on + strand, within MMJJ_RS05005 at 929.986 kb on + strand, within MMJJ_RS05005 at 929.989 kb on - strand, within MMJJ_RS05005 at 930.043 kb on + strand, within MMJJ_RS05005 at 930.043 kb on + strand, within MMJJ_RS05005 at 930.094 kb on - strand, within MMJJ_RS05005 at 930.122 kb on + strand, within MMJJ_RS05005 at 930.138 kb on - strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.139 kb on + strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.176 kb on - strand, within MMJJ_RS05005 at 930.177 kb on + strand, within MMJJ_RS05005 at 930.185 kb on - strand, within MMJJ_RS05005 at 930.185 kb on - strand, within MMJJ_RS05005 at 930.186 kb on + strand, within MMJJ_RS05005 at 930.197 kb on + strand, within MMJJ_RS05005 at 930.210 kb on - strand at 930.246 kb on - strand at 930.269 kb on - strand at 930.284 kb on - strand at 930.284 kb on - strand at 930.284 kb on - strand at 930.285 kb on + strand at 930.304 kb on - strand at 930.304 kb on - strand at 930.305 kb on + strand at 930.308 kb on - strand at 930.309 kb on + strand at 930.309 kb on + strand at 930.309 kb on + strand at 930.323 kb on - strand at 930.338 kb on - strand at 930.385 kb on + strand at 930.395 kb on + strand at 930.421 kb on - strand at 930.429 kb on - strand at 930.438 kb on - strand at 930.444 kb on + strand at 930.461 kb on - strand at 930.461 kb on - strand at 930.476 kb on - strand at 930.505 kb on + strand at 930.525 kb on - strand at 930.533 kb on - strand at 930.534 kb on + strand at 930.544 kb on - strand at 930.560 kb on - strand at 930.569 kb on + strand at 930.604 kb on - strand at 930.729 kb on + strand, within MMJJ_RS05010 at 930.731 kb on - strand, within MMJJ_RS05010 at 930.732 kb on + strand, within MMJJ_RS05010 at 930.796 kb on - strand, within MMJJ_RS05010 at 930.831 kb on - strand, within MMJJ_RS05010 at 930.832 kb on + strand, within MMJJ_RS05010 at 930.832 kb on + strand, within MMJJ_RS05010 at 930.832 kb on + strand, within MMJJ_RS05010 at 930.854 kb on + strand, within MMJJ_RS05010 at 930.863 kb on - strand, within MMJJ_RS05010 at 930.863 kb on - strand, within MMJJ_RS05010 at 930.864 kb on + strand, within MMJJ_RS05010 at 930.875 kb on - strand, within MMJJ_RS05010 at 930.875 kb on - strand, within MMJJ_RS05010 at 930.875 kb on - strand, within MMJJ_RS05010 at 930.875 kb on - strand, within MMJJ_RS05010 at 930.875 kb on - strand, within MMJJ_RS05010 at 930.876 kb on + strand, within MMJJ_RS05010 at 930.876 kb on + strand, within MMJJ_RS05010 at 930.876 kb on + strand, within MMJJ_RS05010 at 930.876 kb on + strand, within MMJJ_RS05010 at 930.876 kb on + strand, within MMJJ_RS05010 at 930.887 kb on - strand, within MMJJ_RS05010 at 930.887 kb on - strand, within MMJJ_RS05010 at 930.950 kb on - strand, within MMJJ_RS05010 at 930.951 kb on + strand, within MMJJ_RS05010 at 930.951 kb on + strand, within MMJJ_RS05010 at 930.967 kb on - strand, within MMJJ_RS05010 at 931.037 kb on - strand, within MMJJ_RS05010 at 931.038 kb on + strand, within MMJJ_RS05010 at 931.075 kb on - strand, within MMJJ_RS05010 at 931.076 kb on + strand, within MMJJ_RS05010 at 931.128 kb on + strand, within MMJJ_RS05010 at 931.128 kb on + strand, within MMJJ_RS05010 at 931.140 kb on + strand, within MMJJ_RS05010 at 931.170 kb on - strand, within MMJJ_RS05010 at 931.170 kb on - strand, within MMJJ_RS05010 at 931.170 kb on - strand, within MMJJ_RS05010 at 931.174 kb on - strand, within MMJJ_RS05010 at 931.175 kb on + strand, within MMJJ_RS05010 at 931.219 kb on + strand, within MMJJ_RS05010 at 931.243 kb on - strand, within MMJJ_RS05010 at 931.255 kb on - strand, within MMJJ_RS05010 at 931.274 kb on - strand, within MMJJ_RS05010 at 931.275 kb on + strand, within MMJJ_RS05010 at 931.331 kb on + strand, within MMJJ_RS05010 at 931.400 kb on + strand, within MMJJ_RS05010 at 931.437 kb on - strand, within MMJJ_RS05010 at 931.437 kb on - strand, within MMJJ_RS05010 at 931.438 kb on + strand, within MMJJ_RS05010 at 931.438 kb on + strand, within MMJJ_RS05010 at 931.455 kb on + strand, within MMJJ_RS05010 at 931.455 kb on + strand, within MMJJ_RS05010 at 931.461 kb on - strand, within MMJJ_RS05010 at 931.469 kb on - strand, within MMJJ_RS05010 at 931.470 kb on + strand, within MMJJ_RS05010 at 931.522 kb on - strand, within MMJJ_RS05010 at 931.525 kb on - strand, within MMJJ_RS05010 at 931.525 kb on - strand, within MMJJ_RS05010 at 931.525 kb on - strand, within MMJJ_RS05010 at 931.525 kb on - strand, within MMJJ_RS05010 at 931.526 kb on + strand, within MMJJ_RS05010 at 931.526 kb on + strand, within MMJJ_RS05010 at 931.526 kb on + strand, within MMJJ_RS05010 at 931.526 kb on + strand, within MMJJ_RS05010 at 931.570 kb on - strand at 931.571 kb on + strand at 931.571 kb on + strand at 931.575 kb on - strand at 931.575 kb on - strand at 931.577 kb on - strand at 931.579 kb on - strand at 931.579 kb on - strand at 931.579 kb on - strand at 931.579 kb on - strand at 931.580 kb on + strand at 931.580 kb on + strand at 931.595 kb on - strand at 931.596 kb on + strand at 931.596 kb on + strand at 931.640 kb on - strand at 931.641 kb on + strand at 931.653 kb on + strand at 931.672 kb on - strand at 931.693 kb on + strand at 931.706 kb on - strand at 931.929 kb on + strand, within MMJJ_RS05015
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 929,593 + -0.3 929,594 - +0.7 929,594 - +1.4 929,594 - +0.4 929,594 - +0.4 929,594 - -0.2 929,594 - -0.4 929,595 + +2.8 929,595 + -0.6 929,597 - +0.6 929,598 + -0.8 929,598 + -1.6 929,614 + MMJJ_RS05005 0.11 -0.0 929,640 - MMJJ_RS05005 0.15 +0.0 929,640 - MMJJ_RS05005 0.15 -0.6 929,642 - MMJJ_RS05005 0.15 +0.0 929,665 - MMJJ_RS05005 0.18 -1.4 929,674 + MMJJ_RS05005 0.19 +0.5 929,683 + MMJJ_RS05005 0.21 +1.0 929,753 + MMJJ_RS05005 0.30 +0.2 929,753 + MMJJ_RS05005 0.30 +0.8 929,796 - MMJJ_RS05005 0.36 +0.6 929,801 - MMJJ_RS05005 0.36 +0.4 929,812 - MMJJ_RS05005 0.38 -0.7 929,827 + MMJJ_RS05005 0.40 +1.6 929,850 - MMJJ_RS05005 0.43 -0.8 929,854 + MMJJ_RS05005 0.44 +1.9 929,854 + MMJJ_RS05005 0.44 -1.5 929,860 - MMJJ_RS05005 0.44 +0.4 929,894 + MMJJ_RS05005 0.49 +2.1 929,986 + MMJJ_RS05005 0.61 +0.7 929,986 + MMJJ_RS05005 0.61 +1.4 929,986 + MMJJ_RS05005 0.61 +1.4 929,989 - MMJJ_RS05005 0.62 -1.6 930,043 + MMJJ_RS05005 0.69 -0.1 930,043 + MMJJ_RS05005 0.69 -0.4 930,094 - MMJJ_RS05005 0.76 -0.3 930,122 + MMJJ_RS05005 0.80 -0.1 930,138 - MMJJ_RS05005 0.82 -0.3 930,139 + MMJJ_RS05005 0.82 -0.6 930,139 + MMJJ_RS05005 0.82 +0.1 930,139 + MMJJ_RS05005 0.82 +1.0 930,139 + MMJJ_RS05005 0.82 +2.1 930,139 + MMJJ_RS05005 0.82 -0.3 930,139 + MMJJ_RS05005 0.82 -0.1 930,139 + MMJJ_RS05005 0.82 +1.8 930,176 - MMJJ_RS05005 0.87 +0.5 930,176 - MMJJ_RS05005 0.87 -0.3 930,176 - MMJJ_RS05005 0.87 -0.2 930,176 - MMJJ_RS05005 0.87 +1.8 930,176 - MMJJ_RS05005 0.87 +0.4 930,176 - MMJJ_RS05005 0.87 +0.5 930,177 + MMJJ_RS05005 0.87 -0.8 930,185 - MMJJ_RS05005 0.88 +2.3 930,185 - MMJJ_RS05005 0.88 +0.3 930,186 + MMJJ_RS05005 0.88 -1.2 930,197 + MMJJ_RS05005 0.90 -0.5 930,210 - +0.8 930,246 - -1.0 930,269 - +1.7 930,284 - -0.9 930,284 - +0.4 930,284 - -1.3 930,285 + +0.4 930,304 - -0.1 930,304 - -0.1 930,305 + -1.1 930,308 - -0.9 930,309 + -0.7 930,309 + +1.3 930,309 + +0.2 930,323 - +2.0 930,338 - +1.6 930,385 + +0.1 930,395 + -0.4 930,421 - +0.2 930,429 - +0.3 930,438 - -0.6 930,444 + +1.1 930,461 - +0.1 930,461 - +0.1 930,476 - +3.6 930,505 + -0.3 930,525 - -0.9 930,533 - -0.2 930,534 + -1.7 930,544 - +1.8 930,560 - -0.5 930,569 + -0.2 930,604 - +1.0 930,729 + MMJJ_RS05010 0.13 +0.9 930,731 - MMJJ_RS05010 0.13 -0.6 930,732 + MMJJ_RS05010 0.13 +1.8 930,796 - MMJJ_RS05010 0.19 +1.4 930,831 - MMJJ_RS05010 0.22 -0.0 930,832 + MMJJ_RS05010 0.22 +0.9 930,832 + MMJJ_RS05010 0.22 +1.5 930,832 + MMJJ_RS05010 0.22 +0.7 930,854 + MMJJ_RS05010 0.24 +0.3 930,863 - MMJJ_RS05010 0.25 +1.3 930,863 - MMJJ_RS05010 0.25 +2.1 930,864 + MMJJ_RS05010 0.25 +0.4 930,875 - MMJJ_RS05010 0.26 +2.3 930,875 - MMJJ_RS05010 0.26 +2.2 930,875 - MMJJ_RS05010 0.26 +0.3 930,875 - MMJJ_RS05010 0.26 -0.2 930,875 - MMJJ_RS05010 0.26 +1.0 930,876 + MMJJ_RS05010 0.26 +1.2 930,876 + MMJJ_RS05010 0.26 -0.7 930,876 + MMJJ_RS05010 0.26 +0.7 930,876 + MMJJ_RS05010 0.26 +1.0 930,876 + MMJJ_RS05010 0.26 -0.1 930,887 - MMJJ_RS05010 0.27 -0.6 930,887 - MMJJ_RS05010 0.27 -1.0 930,950 - MMJJ_RS05010 0.33 +1.8 930,951 + MMJJ_RS05010 0.33 +1.0 930,951 + MMJJ_RS05010 0.33 +2.1 930,967 - MMJJ_RS05010 0.35 +0.8 931,037 - MMJJ_RS05010 0.41 +0.7 931,038 + MMJJ_RS05010 0.41 +0.5 931,075 - MMJJ_RS05010 0.45 +0.7 931,076 + MMJJ_RS05010 0.45 -0.0 931,128 + MMJJ_RS05010 0.50 +1.4 931,128 + MMJJ_RS05010 0.50 +2.3 931,140 + MMJJ_RS05010 0.51 +0.8 931,170 - MMJJ_RS05010 0.53 +1.1 931,170 - MMJJ_RS05010 0.53 +0.9 931,170 - MMJJ_RS05010 0.53 -0.2 931,174 - MMJJ_RS05010 0.54 +0.3 931,175 + MMJJ_RS05010 0.54 -0.3 931,219 + MMJJ_RS05010 0.58 +1.2 931,243 - MMJJ_RS05010 0.60 +0.5 931,255 - MMJJ_RS05010 0.61 +0.6 931,274 - MMJJ_RS05010 0.63 +0.4 931,275 + MMJJ_RS05010 0.63 +1.2 931,331 + MMJJ_RS05010 0.68 -0.2 931,400 + MMJJ_RS05010 0.75 +1.1 931,437 - MMJJ_RS05010 0.78 +0.9 931,437 - MMJJ_RS05010 0.78 +0.1 931,438 + MMJJ_RS05010 0.78 +0.8 931,438 + MMJJ_RS05010 0.78 -0.3 931,455 + MMJJ_RS05010 0.80 -0.5 931,455 + MMJJ_RS05010 0.80 +1.4 931,461 - MMJJ_RS05010 0.80 +0.8 931,469 - MMJJ_RS05010 0.81 +2.0 931,470 + MMJJ_RS05010 0.81 +1.0 931,522 - MMJJ_RS05010 0.86 +0.8 931,525 - MMJJ_RS05010 0.86 +1.2 931,525 - MMJJ_RS05010 0.86 +2.8 931,525 - MMJJ_RS05010 0.86 +0.6 931,525 - MMJJ_RS05010 0.86 +1.8 931,526 + MMJJ_RS05010 0.86 -0.4 931,526 + MMJJ_RS05010 0.86 +0.8 931,526 + MMJJ_RS05010 0.86 +0.1 931,526 + MMJJ_RS05010 0.86 -0.1 931,570 - +1.7 931,571 + -0.0 931,571 + +1.0 931,575 - +1.1 931,575 - -1.4 931,577 - +0.3 931,579 - +1.0 931,579 - -1.7 931,579 - -0.7 931,579 - -0.0 931,580 + +0.0 931,580 + -0.5 931,595 - +0.1 931,596 + -0.4 931,596 + -0.8 931,640 - +0.1 931,641 + +0.5 931,653 + +0.9 931,672 - +0.0 931,693 + +0.7 931,706 - -0.3 931,929 + MMJJ_RS05015 0.29 -0.7
Or see this region's nucleotide sequence