Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02975 and MMJJ_RS02980 are separated by 18 nucleotides MMJJ_RS02980 and MMJJ_RS02985 are separated by 170 nucleotides MMJJ_RS02985 and MMJJ_RS02990 are separated by 55 nucleotides
MMJJ_RS02975: MMJJ_RS02975 - NAD(P)H-dependent oxidoreductase, at 554,450 to 554,944
_RS02975
MMJJ_RS02980: MMJJ_RS02980 - TatD family hydrolase, at 554,963 to 555,724
_RS02980
MMJJ_RS02985: MMJJ_RS02985 - MBL fold metallo-hydrolase, at 555,895 to 557,196
_RS02985
MMJJ_RS02990: MMJJ_RS02990 - hypothetical protein, at 557,252 to 560,617
_RS02990
Position (kb) 555
556
557
558 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.919 kb on - strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.920 kb on + strand at 554.922 kb on + strand at 554.944 kb on - strand at 554.945 kb on + strand at 554.945 kb on + strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.946 kb on - strand at 554.947 kb on + strand at 554.947 kb on + strand at 554.960 kb on - strand at 554.960 kb on - strand at 554.965 kb on + strand at 554.965 kb on + strand at 554.968 kb on - strand at 554.968 kb on - strand at 554.975 kb on + strand at 554.992 kb on - strand at 554.992 kb on - strand at 554.992 kb on - strand at 554.995 kb on - strand at 554.995 kb on - strand at 555.011 kb on - strand at 555.011 kb on - strand at 555.012 kb on + strand at 555.012 kb on + strand at 555.041 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.071 kb on - strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.072 kb on + strand, within MMJJ_RS02980 at 555.094 kb on - strand, within MMJJ_RS02980 at 555.100 kb on - strand, within MMJJ_RS02980 at 555.105 kb on + strand, within MMJJ_RS02980 at 555.138 kb on + strand, within MMJJ_RS02980 at 555.182 kb on + strand, within MMJJ_RS02980 at 555.213 kb on + strand, within MMJJ_RS02980 at 555.243 kb on + strand, within MMJJ_RS02980 at 555.243 kb on + strand, within MMJJ_RS02980 at 555.326 kb on + strand, within MMJJ_RS02980 at 555.344 kb on - strand, within MMJJ_RS02980 at 555.344 kb on - strand, within MMJJ_RS02980 at 555.378 kb on + strand, within MMJJ_RS02980 at 555.465 kb on - strand, within MMJJ_RS02980 at 555.523 kb on - strand, within MMJJ_RS02980 at 555.523 kb on - strand, within MMJJ_RS02980 at 555.524 kb on + strand, within MMJJ_RS02980 at 555.647 kb on + strand, within MMJJ_RS02980 at 555.760 kb on - strand at 555.761 kb on + strand at 555.791 kb on + strand at 555.794 kb on - strand at 555.803 kb on + strand at 555.812 kb on + strand at 555.844 kb on - strand at 555.844 kb on - strand at 555.847 kb on + strand at 555.847 kb on + strand at 555.847 kb on + strand at 555.849 kb on + strand at 555.855 kb on - strand at 555.855 kb on - strand at 555.856 kb on + strand at 555.863 kb on + strand at 555.863 kb on + strand at 555.872 kb on - strand at 555.872 kb on - strand at 555.872 kb on - strand at 555.873 kb on + strand at 555.944 kb on - strand at 555.975 kb on + strand at 556.029 kb on - strand, within MMJJ_RS02985 at 556.110 kb on - strand, within MMJJ_RS02985 at 556.110 kb on - strand, within MMJJ_RS02985 at 556.111 kb on + strand, within MMJJ_RS02985 at 556.126 kb on - strand, within MMJJ_RS02985 at 556.126 kb on - strand, within MMJJ_RS02985 at 556.127 kb on + strand, within MMJJ_RS02985 at 556.233 kb on - strand, within MMJJ_RS02985 at 556.261 kb on + strand, within MMJJ_RS02985 at 556.264 kb on - strand, within MMJJ_RS02985 at 556.347 kb on + strand, within MMJJ_RS02985 at 556.347 kb on + strand, within MMJJ_RS02985 at 556.373 kb on + strand, within MMJJ_RS02985 at 556.409 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.471 kb on - strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.472 kb on + strand, within MMJJ_RS02985 at 556.495 kb on + strand, within MMJJ_RS02985 at 556.562 kb on - strand, within MMJJ_RS02985 at 556.563 kb on + strand, within MMJJ_RS02985 at 556.607 kb on - strand, within MMJJ_RS02985 at 556.610 kb on - strand, within MMJJ_RS02985 at 556.611 kb on + strand, within MMJJ_RS02985 at 556.673 kb on - strand, within MMJJ_RS02985 at 556.673 kb on - strand, within MMJJ_RS02985 at 556.730 kb on - strand, within MMJJ_RS02985 at 556.739 kb on - strand, within MMJJ_RS02985 at 556.739 kb on - strand, within MMJJ_RS02985 at 556.740 kb on + strand, within MMJJ_RS02985 at 556.740 kb on + strand, within MMJJ_RS02985 at 556.769 kb on - strand, within MMJJ_RS02985 at 556.772 kb on - strand, within MMJJ_RS02985 at 556.773 kb on + strand, within MMJJ_RS02985 at 556.799 kb on - strand, within MMJJ_RS02985 at 556.805 kb on - strand, within MMJJ_RS02985 at 556.805 kb on - strand, within MMJJ_RS02985 at 556.806 kb on + strand, within MMJJ_RS02985 at 556.847 kb on + strand, within MMJJ_RS02985 at 556.847 kb on + strand, within MMJJ_RS02985 at 556.847 kb on + strand, within MMJJ_RS02985 at 556.850 kb on - strand, within MMJJ_RS02985 at 556.851 kb on + strand, within MMJJ_RS02985 at 556.861 kb on + strand, within MMJJ_RS02985 at 556.884 kb on + strand, within MMJJ_RS02985 at 556.886 kb on - strand, within MMJJ_RS02985 at 556.994 kb on + strand, within MMJJ_RS02985 at 557.061 kb on + strand, within MMJJ_RS02985 at 557.093 kb on - strand at 557.099 kb on - strand at 557.099 kb on - strand at 557.100 kb on + strand at 557.100 kb on + strand at 557.100 kb on + strand at 557.121 kb on + strand at 557.139 kb on - strand at 557.139 kb on - strand at 557.139 kb on - strand at 557.144 kb on - strand at 557.149 kb on + strand at 557.171 kb on - strand at 557.194 kb on - strand at 557.203 kb on - strand at 557.203 kb on - strand at 557.203 kb on - strand at 557.236 kb on - strand at 557.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 554,919 - -1.6 554,919 - -0.1 554,919 - +0.3 554,919 - -1.0 554,919 - -1.0 554,919 - +0.7 554,919 - -0.6 554,919 - -0.9 554,919 - -0.3 554,920 + +1.3 554,920 + +0.8 554,920 + +0.6 554,920 + -1.3 554,922 + -3.6 554,944 - -1.3 554,945 + -0.1 554,945 + -1.1 554,946 - +0.3 554,946 - -1.0 554,946 - +0.4 554,946 - -0.1 554,947 + +1.6 554,947 + +0.2 554,960 - +1.0 554,960 - -0.3 554,965 + -0.5 554,965 + -2.0 554,968 - -1.2 554,968 - -1.4 554,975 + -0.7 554,992 - -0.4 554,992 - +0.5 554,992 - +0.2 554,995 - +1.1 554,995 - +0.9 555,011 - +0.4 555,011 - +0.6 555,012 + -1.5 555,012 + -0.3 555,041 - MMJJ_RS02980 0.10 +0.7 555,071 - MMJJ_RS02980 0.14 +0.6 555,071 - MMJJ_RS02980 0.14 -0.3 555,071 - MMJJ_RS02980 0.14 -0.5 555,071 - MMJJ_RS02980 0.14 -1.2 555,072 + MMJJ_RS02980 0.14 -0.1 555,072 + MMJJ_RS02980 0.14 +0.7 555,072 + MMJJ_RS02980 0.14 +0.3 555,072 + MMJJ_RS02980 0.14 -0.9 555,072 + MMJJ_RS02980 0.14 -1.2 555,094 - MMJJ_RS02980 0.17 +0.6 555,100 - MMJJ_RS02980 0.18 -0.1 555,105 + MMJJ_RS02980 0.19 -0.6 555,138 + MMJJ_RS02980 0.23 -0.7 555,182 + MMJJ_RS02980 0.29 -1.5 555,213 + MMJJ_RS02980 0.33 +0.6 555,243 + MMJJ_RS02980 0.37 +0.4 555,243 + MMJJ_RS02980 0.37 -1.2 555,326 + MMJJ_RS02980 0.48 -0.4 555,344 - MMJJ_RS02980 0.50 +2.4 555,344 - MMJJ_RS02980 0.50 +1.4 555,378 + MMJJ_RS02980 0.54 -0.4 555,465 - MMJJ_RS02980 0.66 +0.9 555,523 - MMJJ_RS02980 0.73 +0.8 555,523 - MMJJ_RS02980 0.73 -1.5 555,524 + MMJJ_RS02980 0.74 -0.6 555,647 + MMJJ_RS02980 0.90 +0.3 555,760 - -0.4 555,761 + +1.4 555,791 + -1.1 555,794 - -0.7 555,803 + -0.6 555,812 + -0.9 555,844 - +0.0 555,844 - +0.9 555,847 + -2.1 555,847 + +0.3 555,847 + -4.0 555,849 + -2.6 555,855 - -3.5 555,855 - -1.4 555,856 + -1.2 555,863 + -4.1 555,863 + -2.7 555,872 - -3.8 555,872 - -3.8 555,872 - -4.3 555,873 + -2.6 555,944 - -1.5 555,975 + -0.2 556,029 - MMJJ_RS02985 0.10 -3.7 556,110 - MMJJ_RS02985 0.17 -3.4 556,110 - MMJJ_RS02985 0.17 -3.1 556,111 + MMJJ_RS02985 0.17 -3.8 556,126 - MMJJ_RS02985 0.18 -3.8 556,126 - MMJJ_RS02985 0.18 -3.6 556,127 + MMJJ_RS02985 0.18 -3.7 556,233 - MMJJ_RS02985 0.26 -1.1 556,261 + MMJJ_RS02985 0.28 -3.3 556,264 - MMJJ_RS02985 0.28 -4.7 556,347 + MMJJ_RS02985 0.35 -2.9 556,347 + MMJJ_RS02985 0.35 -5.2 556,373 + MMJJ_RS02985 0.37 -4.7 556,409 - MMJJ_RS02985 0.39 -2.5 556,471 - MMJJ_RS02985 0.44 -3.4 556,471 - MMJJ_RS02985 0.44 -4.2 556,471 - MMJJ_RS02985 0.44 -2.7 556,472 + MMJJ_RS02985 0.44 -4.6 556,472 + MMJJ_RS02985 0.44 +0.3 556,472 + MMJJ_RS02985 0.44 -2.1 556,472 + MMJJ_RS02985 0.44 -2.1 556,472 + MMJJ_RS02985 0.44 -5.5 556,472 + MMJJ_RS02985 0.44 -3.6 556,472 + MMJJ_RS02985 0.44 -3.5 556,495 + MMJJ_RS02985 0.46 -5.5 556,562 - MMJJ_RS02985 0.51 -3.6 556,563 + MMJJ_RS02985 0.51 -4.3 556,607 - MMJJ_RS02985 0.55 -0.8 556,610 - MMJJ_RS02985 0.55 -3.3 556,611 + MMJJ_RS02985 0.55 -4.5 556,673 - MMJJ_RS02985 0.60 -2.4 556,673 - MMJJ_RS02985 0.60 -3.8 556,730 - MMJJ_RS02985 0.64 -3.9 556,739 - MMJJ_RS02985 0.65 +0.3 556,739 - MMJJ_RS02985 0.65 -4.3 556,740 + MMJJ_RS02985 0.65 -4.4 556,740 + MMJJ_RS02985 0.65 -4.5 556,769 - MMJJ_RS02985 0.67 -4.0 556,772 - MMJJ_RS02985 0.67 -5.8 556,773 + MMJJ_RS02985 0.67 -2.4 556,799 - MMJJ_RS02985 0.69 -3.9 556,805 - MMJJ_RS02985 0.70 -3.1 556,805 - MMJJ_RS02985 0.70 -3.2 556,806 + MMJJ_RS02985 0.70 -5.7 556,847 + MMJJ_RS02985 0.73 -3.5 556,847 + MMJJ_RS02985 0.73 -1.9 556,847 + MMJJ_RS02985 0.73 -4.5 556,850 - MMJJ_RS02985 0.73 -2.1 556,851 + MMJJ_RS02985 0.73 -3.2 556,861 + MMJJ_RS02985 0.74 -3.2 556,884 + MMJJ_RS02985 0.76 -3.5 556,886 - MMJJ_RS02985 0.76 +0.3 556,994 + MMJJ_RS02985 0.84 -3.0 557,061 + MMJJ_RS02985 0.90 -5.0 557,093 - -2.4 557,099 - -3.1 557,099 - -3.4 557,100 + -5.5 557,100 + -2.6 557,100 + -4.3 557,121 + -4.3 557,139 - -4.5 557,139 - -3.8 557,139 - -2.5 557,144 - -2.7 557,149 + -4.8 557,171 - -3.8 557,194 - -0.2 557,203 - -1.1 557,203 - -1.5 557,203 - -0.6 557,236 - -1.6 557,248 - -0.2
Or see this region's nucleotide sequence