Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS01980 and MMJJ_RS01985 are separated by 26 nucleotides MMJJ_RS01985 and MMJJ_RS01990 are separated by 4 nucleotides MMJJ_RS01990 and MMJJ_RS01995 are separated by 422 nucleotides
MMJJ_RS01980: MMJJ_RS01980 - cation:proton antiporter, at 378,405 to 379,574
_RS01980
MMJJ_RS01985: MMJJ_RS01985 - endonuclease III, at 379,601 to 380,299
_RS01985
MMJJ_RS01990: MMJJ_RS01990 - universal stress protein, at 380,304 to 380,741
_RS01990
MMJJ_RS01995: MMJJ_RS01995 - valine--tRNA ligase, at 381,164 to 383,824
_RS01995
Position (kb) 380
381 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 379.456 kb on - strand, within MMJJ_RS01980 at 379.493 kb on + strand at 379.501 kb on - strand at 379.525 kb on + strand at 379.525 kb on + strand at 379.525 kb on + strand at 379.568 kb on + strand at 379.590 kb on + strand at 379.607 kb on - strand at 379.607 kb on - strand at 379.608 kb on + strand at 379.641 kb on - strand at 379.686 kb on - strand, within MMJJ_RS01985 at 379.687 kb on + strand, within MMJJ_RS01985 at 379.687 kb on + strand, within MMJJ_RS01985 at 379.696 kb on - strand, within MMJJ_RS01985 at 379.697 kb on + strand at 379.697 kb on + strand, within MMJJ_RS01985 at 379.697 kb on + strand, within MMJJ_RS01985 at 379.719 kb on - strand, within MMJJ_RS01985 at 379.738 kb on - strand, within MMJJ_RS01985 at 379.739 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.743 kb on + strand, within MMJJ_RS01985 at 379.797 kb on - strand, within MMJJ_RS01985 at 379.845 kb on - strand, within MMJJ_RS01985 at 379.855 kb on + strand, within MMJJ_RS01985 at 379.858 kb on + strand, within MMJJ_RS01985 at 379.863 kb on - strand, within MMJJ_RS01985 at 379.864 kb on + strand, within MMJJ_RS01985 at 379.864 kb on + strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.882 kb on - strand, within MMJJ_RS01985 at 379.883 kb on + strand, within MMJJ_RS01985 at 379.883 kb on + strand, within MMJJ_RS01985 at 379.902 kb on - strand, within MMJJ_RS01985 at 379.954 kb on + strand, within MMJJ_RS01985 at 379.983 kb on - strand, within MMJJ_RS01985 at 380.019 kb on - strand, within MMJJ_RS01985 at 380.060 kb on - strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.061 kb on + strand, within MMJJ_RS01985 at 380.068 kb on - strand, within MMJJ_RS01985 at 380.092 kb on - strand, within MMJJ_RS01985 at 380.092 kb on - strand, within MMJJ_RS01985 at 380.093 kb on + strand, within MMJJ_RS01985 at 380.093 kb on + strand, within MMJJ_RS01985 at 380.096 kb on - strand at 380.096 kb on - strand, within MMJJ_RS01985 at 380.096 kb on - strand, within MMJJ_RS01985 at 380.097 kb on + strand, within MMJJ_RS01985 at 380.097 kb on + strand, within MMJJ_RS01985 at 380.098 kb on - strand, within MMJJ_RS01985 at 380.098 kb on - strand, within MMJJ_RS01985 at 380.128 kb on + strand, within MMJJ_RS01985 at 380.199 kb on - strand, within MMJJ_RS01985 at 380.200 kb on + strand, within MMJJ_RS01985 at 380.200 kb on + strand, within MMJJ_RS01985 at 380.243 kb on - strand at 380.243 kb on - strand at 380.284 kb on + strand at 380.290 kb on + strand at 380.297 kb on - strand at 380.297 kb on - strand at 380.298 kb on + strand at 380.303 kb on + strand at 380.328 kb on - strand at 380.328 kb on - strand at 380.379 kb on - strand, within MMJJ_RS01990 at 380.409 kb on + strand, within MMJJ_RS01990 at 380.421 kb on - strand, within MMJJ_RS01990 at 380.436 kb on + strand, within MMJJ_RS01990 at 380.436 kb on + strand, within MMJJ_RS01990 at 380.625 kb on - strand, within MMJJ_RS01990 at 380.625 kb on - strand, within MMJJ_RS01990 at 380.626 kb on + strand, within MMJJ_RS01990 at 380.629 kb on - strand, within MMJJ_RS01990 at 380.691 kb on - strand, within MMJJ_RS01990 at 380.692 kb on + strand, within MMJJ_RS01990 at 380.692 kb on + strand, within MMJJ_RS01990 at 380.721 kb on + strand at 380.732 kb on - strand at 380.735 kb on + strand at 380.758 kb on + strand at 380.766 kb on - strand at 380.767 kb on + strand at 380.767 kb on + strand at 380.767 kb on + strand at 380.773 kb on + strand at 380.784 kb on - strand at 380.785 kb on + strand at 380.785 kb on + strand at 380.786 kb on - strand at 380.786 kb on - strand at 380.786 kb on - strand at 380.786 kb on - strand at 380.787 kb on + strand at 380.787 kb on + strand at 380.787 kb on + strand at 380.787 kb on + strand at 380.788 kb on - strand at 380.789 kb on + strand at 380.789 kb on + strand at 380.789 kb on + strand at 380.791 kb on - strand at 380.791 kb on - strand at 380.792 kb on + strand at 380.792 kb on + strand at 380.794 kb on - strand at 380.798 kb on - strand at 380.798 kb on - strand at 380.799 kb on + strand at 380.811 kb on - strand at 380.825 kb on - strand at 380.826 kb on + strand at 380.844 kb on - strand at 380.844 kb on - strand at 380.845 kb on + strand at 380.848 kb on + strand at 380.853 kb on - strand at 380.854 kb on + strand at 380.872 kb on - strand at 380.875 kb on - strand at 380.875 kb on - strand at 380.875 kb on - strand at 380.875 kb on - strand at 380.875 kb on - strand at 380.876 kb on + strand at 380.876 kb on + strand at 380.876 kb on + strand at 380.876 kb on + strand at 380.877 kb on - strand at 380.877 kb on - strand at 380.878 kb on + strand at 380.878 kb on + strand at 380.878 kb on + strand at 380.878 kb on + strand at 380.879 kb on - strand at 380.880 kb on + strand at 380.880 kb on + strand at 380.887 kb on + strand at 380.890 kb on - strand at 380.890 kb on - strand at 380.890 kb on - strand at 380.894 kb on - strand at 380.894 kb on - strand at 380.904 kb on + strand at 380.904 kb on + strand at 380.912 kb on - strand at 380.913 kb on + strand at 380.914 kb on - strand at 380.915 kb on + strand at 380.915 kb on + strand at 380.915 kb on + strand at 380.921 kb on - strand at 380.930 kb on - strand at 380.992 kb on + strand at 380.992 kb on + strand at 381.014 kb on + strand at 381.029 kb on + strand at 381.034 kb on - strand at 381.035 kb on + strand at 381.099 kb on - strand at 381.100 kb on + strand at 381.584 kb on + strand, within MMJJ_RS01995
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 379,456 - MMJJ_RS01980 0.90 +0.0 379,493 + -0.1 379,501 - -0.2 379,525 + -0.8 379,525 + +0.9 379,525 + -0.7 379,568 + +0.4 379,590 + +1.7 379,607 - -0.4 379,607 - -0.3 379,608 + +0.7 379,641 - +0.3 379,686 - MMJJ_RS01985 0.12 +0.1 379,687 + MMJJ_RS01985 0.12 +0.9 379,687 + MMJJ_RS01985 0.12 +0.4 379,696 - MMJJ_RS01985 0.14 -0.7 379,697 + +0.3 379,697 + MMJJ_RS01985 0.14 -2.4 379,697 + MMJJ_RS01985 0.14 -0.6 379,719 - MMJJ_RS01985 0.17 -1.8 379,738 - MMJJ_RS01985 0.20 +0.2 379,739 + MMJJ_RS01985 0.20 -0.8 379,743 + MMJJ_RS01985 0.20 +0.0 379,743 + MMJJ_RS01985 0.20 +0.6 379,743 + MMJJ_RS01985 0.20 -0.1 379,797 - MMJJ_RS01985 0.28 -0.4 379,845 - MMJJ_RS01985 0.35 -0.1 379,855 + MMJJ_RS01985 0.36 +0.1 379,858 + MMJJ_RS01985 0.37 +0.3 379,863 - MMJJ_RS01985 0.37 -0.2 379,864 + MMJJ_RS01985 0.38 -0.6 379,864 + MMJJ_RS01985 0.38 +0.1 379,882 - MMJJ_RS01985 0.40 -1.1 379,882 - MMJJ_RS01985 0.40 -0.3 379,882 - MMJJ_RS01985 0.40 -0.6 379,883 + MMJJ_RS01985 0.40 -1.0 379,883 + MMJJ_RS01985 0.40 -0.5 379,902 - MMJJ_RS01985 0.43 +1.6 379,954 + MMJJ_RS01985 0.51 +0.4 379,983 - MMJJ_RS01985 0.55 +1.1 380,019 - MMJJ_RS01985 0.60 +0.9 380,060 - MMJJ_RS01985 0.66 -0.5 380,061 + MMJJ_RS01985 0.66 +2.2 380,061 + MMJJ_RS01985 0.66 -1.2 380,061 + MMJJ_RS01985 0.66 -1.0 380,068 - MMJJ_RS01985 0.67 +1.1 380,092 - MMJJ_RS01985 0.70 +0.4 380,092 - MMJJ_RS01985 0.70 +0.3 380,093 + MMJJ_RS01985 0.70 +0.3 380,093 + MMJJ_RS01985 0.70 +1.8 380,096 - +0.7 380,096 - MMJJ_RS01985 0.71 +0.5 380,096 - MMJJ_RS01985 0.71 -1.2 380,097 + MMJJ_RS01985 0.71 -3.6 380,097 + MMJJ_RS01985 0.71 -0.2 380,098 - MMJJ_RS01985 0.71 -0.7 380,098 - MMJJ_RS01985 0.71 +1.3 380,128 + MMJJ_RS01985 0.75 -1.4 380,199 - MMJJ_RS01985 0.86 +0.9 380,200 + MMJJ_RS01985 0.86 -0.1 380,200 + MMJJ_RS01985 0.86 +0.6 380,243 - +1.2 380,243 - -0.8 380,284 + -0.4 380,290 + -1.9 380,297 - -2.1 380,297 - -0.3 380,298 + -1.3 380,303 + -0.9 380,328 - -4.5 380,328 - -3.4 380,379 - MMJJ_RS01990 0.17 -4.1 380,409 + MMJJ_RS01990 0.24 -4.7 380,421 - MMJJ_RS01990 0.27 -1.1 380,436 + MMJJ_RS01990 0.30 -3.6 380,436 + MMJJ_RS01990 0.30 -3.4 380,625 - MMJJ_RS01990 0.73 -2.4 380,625 - MMJJ_RS01990 0.73 -3.1 380,626 + MMJJ_RS01990 0.74 -1.0 380,629 - MMJJ_RS01990 0.74 -3.5 380,691 - MMJJ_RS01990 0.88 -1.3 380,692 + MMJJ_RS01990 0.89 -2.6 380,692 + MMJJ_RS01990 0.89 +0.3 380,721 + -2.8 380,732 - -3.2 380,735 + -2.3 380,758 + -2.7 380,766 - -3.6 380,767 + -4.4 380,767 + -3.1 380,767 + -2.9 380,773 + -3.0 380,784 - -2.7 380,785 + -4.0 380,785 + -1.9 380,786 - -2.1 380,786 - -3.1 380,786 - -0.3 380,786 - -3.3 380,787 + -1.5 380,787 + -4.2 380,787 + -3.1 380,787 + -1.3 380,788 - -3.2 380,789 + -2.5 380,789 + -1.7 380,789 + -2.3 380,791 - -3.8 380,791 - -4.0 380,792 + -3.1 380,792 + -3.1 380,794 - -3.5 380,798 - -2.4 380,798 - -3.0 380,799 + -1.6 380,811 - +0.1 380,825 - -0.2 380,826 + -0.0 380,844 - -0.5 380,844 - -1.3 380,845 + -1.6 380,848 + -1.4 380,853 - -1.7 380,854 + -2.2 380,872 - -0.1 380,875 - -0.9 380,875 - +0.4 380,875 - +0.2 380,875 - -1.5 380,875 - +0.6 380,876 + -0.1 380,876 + -1.9 380,876 + -0.2 380,876 + -3.0 380,877 - +2.1 380,877 - +1.5 380,878 + +3.3 380,878 + -0.2 380,878 + -1.3 380,878 + +0.1 380,879 - -0.2 380,880 + -0.4 380,880 + +1.0 380,887 + -0.2 380,890 - +0.7 380,890 - -0.4 380,890 - -1.3 380,894 - -0.1 380,894 - -0.6 380,904 + +0.5 380,904 + +0.4 380,912 - -3.7 380,913 + -1.8 380,914 - -2.8 380,915 + -2.9 380,915 + -0.5 380,915 + -0.9 380,921 - -2.7 380,930 - -1.9 380,992 + -2.7 380,992 + -0.7 381,014 + -1.5 381,029 + -3.1 381,034 - -0.3 381,035 + -1.3 381,099 - -1.3 381,100 + -0.7 381,584 + MMJJ_RS01995 0.16 -1.0
Or see this region's nucleotide sequence