Experiment: CDM1 without Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15365 and BBR_RS15370 are separated by 74 nucleotides BBR_RS15370 and BBR_RS15375 are separated by 20 nucleotides
BBR_RS15365: BBR_RS15365 - alpha/beta hydrolase, at 1,218,000 to 1,218,935
_RS15365
BBR_RS15370: BBR_RS15370 - macro domain-containing protein, at 1,219,010 to 1,219,519
_RS15370
BBR_RS15375: BBR_RS15375 - phosphoesterase, at 1,219,540 to 1,220,283
_RS15375
Position (kb)
1219
1220 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1218.100 kb on - strand, within BBR_RS15365 at 1218.108 kb on + strand, within BBR_RS15365 at 1218.150 kb on + strand, within BBR_RS15365 at 1218.152 kb on + strand, within BBR_RS15365 at 1218.185 kb on - strand, within BBR_RS15365 at 1218.198 kb on + strand, within BBR_RS15365 at 1218.206 kb on - strand, within BBR_RS15365 at 1218.211 kb on + strand, within BBR_RS15365 at 1218.211 kb on + strand, within BBR_RS15365 at 1218.228 kb on + strand, within BBR_RS15365 at 1218.273 kb on + strand, within BBR_RS15365 at 1218.273 kb on + strand, within BBR_RS15365 at 1218.324 kb on + strand, within BBR_RS15365 at 1218.328 kb on + strand, within BBR_RS15365 at 1218.388 kb on - strand, within BBR_RS15365 at 1218.461 kb on + strand, within BBR_RS15365 at 1218.465 kb on + strand, within BBR_RS15365 at 1218.599 kb on + strand, within BBR_RS15365 at 1218.644 kb on + strand, within BBR_RS15365 at 1218.667 kb on + strand, within BBR_RS15365 at 1218.668 kb on - strand, within BBR_RS15365 at 1218.668 kb on - strand, within BBR_RS15365 at 1218.712 kb on + strand, within BBR_RS15365 at 1218.769 kb on - strand, within BBR_RS15365 at 1218.893 kb on - strand at 1219.101 kb on + strand, within BBR_RS15370 at 1219.101 kb on + strand, within BBR_RS15370 at 1219.218 kb on - strand, within BBR_RS15370 at 1219.247 kb on + strand, within BBR_RS15370 at 1219.354 kb on + strand, within BBR_RS15370 at 1219.387 kb on - strand, within BBR_RS15370 at 1219.391 kb on - strand, within BBR_RS15370 at 1219.670 kb on - strand, within BBR_RS15375 at 1219.724 kb on + strand, within BBR_RS15375 at 1219.724 kb on + strand, within BBR_RS15375 at 1219.757 kb on + strand, within BBR_RS15375 at 1219.830 kb on - strand, within BBR_RS15375 at 1219.848 kb on - strand, within BBR_RS15375 at 1219.880 kb on - strand, within BBR_RS15375 at 1219.890 kb on + strand, within BBR_RS15375 at 1219.968 kb on + strand, within BBR_RS15375 at 1219.968 kb on + strand, within BBR_RS15375 at 1220.034 kb on - strand, within BBR_RS15375 at 1220.090 kb on + strand, within BBR_RS15375 at 1220.152 kb on - strand, within BBR_RS15375 at 1220.169 kb on + strand, within BBR_RS15375 at 1220.169 kb on + strand, within BBR_RS15375 at 1220.196 kb on - strand, within BBR_RS15375 at 1220.225 kb on - strand at 1220.225 kb on - strand at 1220.300 kb on + strand at 1220.308 kb on - strand at 1220.308 kb on - strand at 1220.328 kb on - strand at 1220.348 kb on - strand at 1220.376 kb on - strand at 1220.402 kb on - strand at 1220.448 kb on - strand at 1220.471 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Asparagine remove 1,218,100 - BBR_RS15365 0.11 -1.2 1,218,108 + BBR_RS15365 0.12 -2.0 1,218,150 + BBR_RS15365 0.16 -2.0 1,218,152 + BBR_RS15365 0.16 -1.0 1,218,185 - BBR_RS15365 0.20 -2.2 1,218,198 + BBR_RS15365 0.21 +2.1 1,218,206 - BBR_RS15365 0.22 -1.6 1,218,211 + BBR_RS15365 0.23 -3.1 1,218,211 + BBR_RS15365 0.23 -2.8 1,218,228 + BBR_RS15365 0.24 -0.9 1,218,273 + BBR_RS15365 0.29 -1.0 1,218,273 + BBR_RS15365 0.29 +0.9 1,218,324 + BBR_RS15365 0.35 +0.2 1,218,328 + BBR_RS15365 0.35 -1.3 1,218,388 - BBR_RS15365 0.41 -3.1 1,218,461 + BBR_RS15365 0.49 -0.2 1,218,465 + BBR_RS15365 0.50 +0.5 1,218,599 + BBR_RS15365 0.64 -0.2 1,218,644 + BBR_RS15365 0.69 +1.2 1,218,667 + BBR_RS15365 0.71 -0.9 1,218,668 - BBR_RS15365 0.71 -1.0 1,218,668 - BBR_RS15365 0.71 -1.2 1,218,712 + BBR_RS15365 0.76 -0.6 1,218,769 - BBR_RS15365 0.82 -0.1 1,218,893 - -0.9 1,219,101 + BBR_RS15370 0.18 -0.6 1,219,101 + BBR_RS15370 0.18 -3.1 1,219,218 - BBR_RS15370 0.41 -1.6 1,219,247 + BBR_RS15370 0.46 -1.6 1,219,354 + BBR_RS15370 0.67 -2.1 1,219,387 - BBR_RS15370 0.74 -1.5 1,219,391 - BBR_RS15370 0.75 -0.7 1,219,670 - BBR_RS15375 0.17 +0.0 1,219,724 + BBR_RS15375 0.25 -3.0 1,219,724 + BBR_RS15375 0.25 -1.3 1,219,757 + BBR_RS15375 0.29 -2.1 1,219,830 - BBR_RS15375 0.39 -0.6 1,219,848 - BBR_RS15375 0.41 -3.1 1,219,880 - BBR_RS15375 0.46 -2.2 1,219,890 + BBR_RS15375 0.47 -0.8 1,219,968 + BBR_RS15375 0.58 +0.6 1,219,968 + BBR_RS15375 0.58 -0.4 1,220,034 - BBR_RS15375 0.66 +0.9 1,220,090 + BBR_RS15375 0.74 -0.9 1,220,152 - BBR_RS15375 0.82 -1.8 1,220,169 + BBR_RS15375 0.85 -1.3 1,220,169 + BBR_RS15375 0.85 -1.2 1,220,196 - BBR_RS15375 0.88 -0.2 1,220,225 - -3.0 1,220,225 - -3.1 1,220,300 + -0.0 1,220,308 - -1.1 1,220,308 - -0.1 1,220,328 - -3.2 1,220,348 - -0.6 1,220,376 - -1.2 1,220,402 - -0.6 1,220,448 - -3.4 1,220,471 + -1.5
Or see this region's nucleotide sequence