Experiment: CDM1 without Asparagine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12530 and BBR_RS20715 are separated by 10 nucleotides BBR_RS20715 and BBR_RS12535 are separated by 86 nucleotides BBR_RS12535 and BBR_RS12540 are separated by 40 nucleotides
BBR_RS12530: BBR_RS12530 - HNH endonuclease, at 566,793 to 567,551
_RS12530
BBR_RS20715: BBR_RS20715 - hypothetical protein, at 567,562 to 567,885
_RS20715
BBR_RS12535: BBR_RS12535 - divalent metal cation transporter MntH, at 567,972 to 569,297
_RS12535
BBR_RS12540: BBR_RS12540 - MFS transporter, at 569,338 to 570,936
_RS12540
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 566.975 kb on - strand, within BBR_RS12530 at 567.007 kb on - strand, within BBR_RS12530 at 567.012 kb on + strand, within BBR_RS12530 at 567.030 kb on + strand, within BBR_RS12530 at 567.162 kb on + strand, within BBR_RS12530 at 567.173 kb on - strand, within BBR_RS12530 at 567.175 kb on + strand, within BBR_RS12530 at 567.179 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.185 kb on + strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.193 kb on - strand, within BBR_RS12530 at 567.203 kb on - strand, within BBR_RS12530 at 567.235 kb on + strand, within BBR_RS12530 at 567.240 kb on - strand, within BBR_RS12530 at 567.296 kb on - strand, within BBR_RS12530 at 567.324 kb on + strand, within BBR_RS12530 at 567.334 kb on + strand, within BBR_RS12530 at 567.334 kb on + strand, within BBR_RS12530 at 567.349 kb on + strand, within BBR_RS12530 at 567.357 kb on - strand, within BBR_RS12530 at 567.386 kb on - strand, within BBR_RS12530 at 567.393 kb on + strand, within BBR_RS12530 at 567.393 kb on + strand, within BBR_RS12530 at 567.423 kb on - strand, within BBR_RS12530 at 567.513 kb on + strand at 567.513 kb on + strand at 567.518 kb on - strand at 567.520 kb on - strand at 567.522 kb on + strand at 567.546 kb on + strand at 567.546 kb on + strand at 567.546 kb on + strand at 567.554 kb on - strand at 567.559 kb on + strand at 567.562 kb on - strand at 567.562 kb on - strand at 567.580 kb on + strand at 567.585 kb on - strand at 567.590 kb on - strand at 567.592 kb on - strand at 567.651 kb on + strand, within BBR_RS20715 at 567.661 kb on - strand, within BBR_RS20715 at 567.663 kb on - strand, within BBR_RS20715 at 567.670 kb on + strand, within BBR_RS20715 at 567.723 kb on + strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.731 kb on - strand, within BBR_RS20715 at 567.918 kb on + strand at 567.929 kb on + strand at 567.937 kb on - strand at 567.958 kb on - strand at 567.968 kb on + strand at 567.976 kb on - strand at 567.989 kb on - strand at 568.147 kb on - strand, within BBR_RS12535 at 568.151 kb on - strand, within BBR_RS12535 at 568.196 kb on - strand, within BBR_RS12535 at 568.202 kb on + strand, within BBR_RS12535 at 568.203 kb on - strand, within BBR_RS12535 at 568.205 kb on + strand, within BBR_RS12535 at 568.210 kb on + strand, within BBR_RS12535 at 568.210 kb on + strand, within BBR_RS12535 at 568.223 kb on + strand, within BBR_RS12535 at 568.246 kb on + strand, within BBR_RS12535 at 568.246 kb on + strand, within BBR_RS12535 at 568.254 kb on - strand, within BBR_RS12535 at 568.254 kb on - strand, within BBR_RS12535 at 568.280 kb on + strand, within BBR_RS12535 at 568.296 kb on + strand, within BBR_RS12535 at 568.319 kb on - strand, within BBR_RS12535 at 568.339 kb on + strand, within BBR_RS12535 at 568.349 kb on + strand, within BBR_RS12535 at 568.356 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.357 kb on - strand, within BBR_RS12535 at 568.423 kb on - strand, within BBR_RS12535 at 568.475 kb on - strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.481 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.502 kb on + strand, within BBR_RS12535 at 568.504 kb on + strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.515 kb on - strand, within BBR_RS12535 at 568.547 kb on - strand, within BBR_RS12535 at 568.568 kb on + strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.576 kb on - strand, within BBR_RS12535 at 568.604 kb on - strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.619 kb on + strand, within BBR_RS12535 at 568.634 kb on - strand, within BBR_RS12535 at 568.638 kb on + strand, within BBR_RS12535 at 568.648 kb on - strand, within BBR_RS12535 at 568.692 kb on - strand, within BBR_RS12535 at 568.696 kb on + strand, within BBR_RS12535 at 568.714 kb on + strand, within BBR_RS12535 at 568.716 kb on + strand, within BBR_RS12535 at 568.717 kb on + strand, within BBR_RS12535 at 568.736 kb on + strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.762 kb on - strand, within BBR_RS12535 at 568.786 kb on - strand, within BBR_RS12535 at 568.827 kb on - strand, within BBR_RS12535 at 568.842 kb on + strand, within BBR_RS12535 at 568.898 kb on + strand, within BBR_RS12535 at 568.965 kb on - strand, within BBR_RS12535 at 569.004 kb on + strand, within BBR_RS12535 at 569.024 kb on + strand, within BBR_RS12535 at 569.032 kb on - strand, within BBR_RS12535 at 569.033 kb on - strand, within BBR_RS12535 at 569.035 kb on + strand, within BBR_RS12535 at 569.038 kb on - strand, within BBR_RS12535 at 569.038 kb on - strand, within BBR_RS12535 at 569.053 kb on + strand, within BBR_RS12535 at 569.077 kb on - strand, within BBR_RS12535 at 569.077 kb on - strand, within BBR_RS12535 at 569.113 kb on + strand, within BBR_RS12535 at 569.130 kb on + strand, within BBR_RS12535 at 569.130 kb on + strand, within BBR_RS12535 at 569.143 kb on + strand, within BBR_RS12535 at 569.151 kb on - strand, within BBR_RS12535 at 569.189 kb on + strand at 569.189 kb on - strand at 569.189 kb on - strand at 569.228 kb on - strand at 569.272 kb on - strand at 569.277 kb on + strand at 569.279 kb on - strand at 569.279 kb on - strand at 569.285 kb on - strand at 569.313 kb on + strand at 569.343 kb on + strand at 569.353 kb on - strand at 569.353 kb on - strand at 569.353 kb on - strand at 569.392 kb on - strand at 569.406 kb on - strand at 569.411 kb on - strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on + strand at 569.463 kb on - strand at 569.471 kb on - strand at 569.471 kb on - strand at 569.481 kb on - strand at 569.505 kb on - strand, within BBR_RS12540 at 569.541 kb on + strand, within BBR_RS12540 at 569.571 kb on - strand, within BBR_RS12540 at 569.606 kb on + strand, within BBR_RS12540 at 569.608 kb on + strand, within BBR_RS12540 at 569.645 kb on - strand, within BBR_RS12540 at 569.645 kb on - strand, within BBR_RS12540 at 569.775 kb on + strand, within BBR_RS12540 at 569.775 kb on - strand, within BBR_RS12540 at 569.868 kb on + strand, within BBR_RS12540 at 569.876 kb on - strand, within BBR_RS12540 at 569.897 kb on - strand, within BBR_RS12540 at 569.955 kb on + strand, within BBR_RS12540 at 569.955 kb on + strand, within BBR_RS12540 at 570.003 kb on + strand, within BBR_RS12540 at 570.013 kb on - strand, within BBR_RS12540 at 570.013 kb on - strand, within BBR_RS12540 at 570.035 kb on - strand, within BBR_RS12540 at 570.035 kb on - strand, within BBR_RS12540 at 570.042 kb on + strand, within BBR_RS12540 at 570.047 kb on + strand, within BBR_RS12540 at 570.055 kb on - strand, within BBR_RS12540 at 570.059 kb on + strand, within BBR_RS12540 at 570.108 kb on - strand, within BBR_RS12540 at 570.143 kb on + strand, within BBR_RS12540 at 570.143 kb on + strand, within BBR_RS12540 at 570.151 kb on - strand, within BBR_RS12540 at 570.151 kb on - strand, within BBR_RS12540 at 570.162 kb on - strand, within BBR_RS12540 at 570.162 kb on - strand, within BBR_RS12540 at 570.168 kb on + strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.176 kb on - strand, within BBR_RS12540 at 570.217 kb on + strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540 at 570.260 kb on - strand, within BBR_RS12540
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Asparagine remove 566,975 - BBR_RS12530 0.24 -1.1 567,007 - BBR_RS12530 0.28 -0.1 567,012 + BBR_RS12530 0.29 -1.9 567,030 + BBR_RS12530 0.31 +0.6 567,162 + BBR_RS12530 0.49 +0.4 567,173 - BBR_RS12530 0.50 +0.7 567,175 + BBR_RS12530 0.50 +0.1 567,179 + BBR_RS12530 0.51 -0.8 567,185 + BBR_RS12530 0.52 -2.0 567,185 + BBR_RS12530 0.52 +1.2 567,185 + BBR_RS12530 0.52 -0.7 567,185 + BBR_RS12530 0.52 -1.4 567,185 + BBR_RS12530 0.52 -0.0 567,185 + BBR_RS12530 0.52 +1.1 567,185 + BBR_RS12530 0.52 -1.2 567,185 + BBR_RS12530 0.52 -0.2 567,185 + BBR_RS12530 0.52 +0.4 567,185 + BBR_RS12530 0.52 -0.9 567,185 + BBR_RS12530 0.52 -1.6 567,185 + BBR_RS12530 0.52 +0.8 567,185 + BBR_RS12530 0.52 +0.2 567,193 - BBR_RS12530 0.53 +1.2 567,193 - BBR_RS12530 0.53 -0.8 567,193 - BBR_RS12530 0.53 -0.8 567,193 - BBR_RS12530 0.53 -0.2 567,193 - BBR_RS12530 0.53 +0.8 567,193 - BBR_RS12530 0.53 +0.1 567,193 - BBR_RS12530 0.53 +1.5 567,193 - BBR_RS12530 0.53 +1.6 567,193 - BBR_RS12530 0.53 -2.0 567,193 - BBR_RS12530 0.53 +0.3 567,203 - BBR_RS12530 0.54 -0.6 567,235 + BBR_RS12530 0.58 -0.4 567,240 - BBR_RS12530 0.59 +0.7 567,296 - BBR_RS12530 0.66 +1.0 567,324 + BBR_RS12530 0.70 +0.2 567,334 + BBR_RS12530 0.71 -2.0 567,334 + BBR_RS12530 0.71 -1.5 567,349 + BBR_RS12530 0.73 +0.9 567,357 - BBR_RS12530 0.74 +2.4 567,386 - BBR_RS12530 0.78 +0.7 567,393 + BBR_RS12530 0.79 -2.0 567,393 + BBR_RS12530 0.79 -2.3 567,423 - BBR_RS12530 0.83 -0.2 567,513 + -0.3 567,513 + +2.6 567,518 - -0.2 567,520 - -1.9 567,522 + +0.9 567,546 + +0.3 567,546 + +0.9 567,546 + -0.1 567,554 - +1.1 567,559 + -3.1 567,562 - +0.0 567,562 - +0.4 567,580 + +0.0 567,585 - +0.8 567,590 - +1.1 567,592 - -2.1 567,651 + BBR_RS20715 0.27 -2.4 567,661 - BBR_RS20715 0.31 -0.4 567,663 - BBR_RS20715 0.31 -1.5 567,670 + BBR_RS20715 0.33 -1.0 567,723 + BBR_RS20715 0.50 +0.1 567,731 - BBR_RS20715 0.52 +0.6 567,731 - BBR_RS20715 0.52 +0.9 567,731 - BBR_RS20715 0.52 -2.4 567,731 - BBR_RS20715 0.52 +0.5 567,918 + -1.8 567,929 + -1.8 567,937 - +1.1 567,958 - -1.3 567,968 + -3.7 567,976 - +0.2 567,989 - -0.1 568,147 - BBR_RS12535 0.13 -3.1 568,151 - BBR_RS12535 0.13 -2.7 568,196 - BBR_RS12535 0.17 -3.8 568,202 + BBR_RS12535 0.17 +0.5 568,203 - BBR_RS12535 0.17 -0.4 568,205 + BBR_RS12535 0.18 -2.0 568,210 + BBR_RS12535 0.18 -0.6 568,210 + BBR_RS12535 0.18 -2.5 568,223 + BBR_RS12535 0.19 +0.6 568,246 + BBR_RS12535 0.21 +1.9 568,246 + BBR_RS12535 0.21 -0.3 568,254 - BBR_RS12535 0.21 -2.2 568,254 - BBR_RS12535 0.21 -2.5 568,280 + BBR_RS12535 0.23 -1.5 568,296 + BBR_RS12535 0.24 +0.3 568,319 - BBR_RS12535 0.26 -2.8 568,339 + BBR_RS12535 0.28 -1.3 568,349 + BBR_RS12535 0.28 -3.0 568,356 - BBR_RS12535 0.29 -2.8 568,357 - BBR_RS12535 0.29 -3.4 568,357 - BBR_RS12535 0.29 -0.3 568,357 - BBR_RS12535 0.29 -2.1 568,423 - BBR_RS12535 0.34 -1.6 568,475 - BBR_RS12535 0.38 -2.8 568,481 + BBR_RS12535 0.38 -0.7 568,481 + BBR_RS12535 0.38 +0.7 568,502 + BBR_RS12535 0.40 +1.0 568,502 + BBR_RS12535 0.40 -0.8 568,504 + BBR_RS12535 0.40 -3.6 568,515 - BBR_RS12535 0.41 -3.5 568,515 - BBR_RS12535 0.41 -1.7 568,547 - BBR_RS12535 0.43 -2.8 568,568 + BBR_RS12535 0.45 -2.1 568,576 - BBR_RS12535 0.46 -1.6 568,576 - BBR_RS12535 0.46 -2.1 568,604 - BBR_RS12535 0.48 -1.1 568,619 + BBR_RS12535 0.49 -2.4 568,619 + BBR_RS12535 0.49 -2.0 568,634 - BBR_RS12535 0.50 -2.0 568,638 + BBR_RS12535 0.50 -2.4 568,648 - BBR_RS12535 0.51 -2.9 568,692 - BBR_RS12535 0.54 -2.2 568,696 + BBR_RS12535 0.55 -1.6 568,714 + BBR_RS12535 0.56 -0.3 568,716 + BBR_RS12535 0.56 -1.0 568,717 + BBR_RS12535 0.56 +0.9 568,736 + BBR_RS12535 0.58 -3.7 568,762 - BBR_RS12535 0.60 -2.1 568,762 - BBR_RS12535 0.60 -4.2 568,786 - BBR_RS12535 0.61 -0.6 568,827 - BBR_RS12535 0.64 -3.3 568,842 + BBR_RS12535 0.66 -3.1 568,898 + BBR_RS12535 0.70 -0.9 568,965 - BBR_RS12535 0.75 -2.6 569,004 + BBR_RS12535 0.78 -0.6 569,024 + BBR_RS12535 0.79 -0.1 569,032 - BBR_RS12535 0.80 -1.2 569,033 - BBR_RS12535 0.80 -3.1 569,035 + BBR_RS12535 0.80 -2.0 569,038 - BBR_RS12535 0.80 -3.6 569,038 - BBR_RS12535 0.80 -3.2 569,053 + BBR_RS12535 0.82 -3.7 569,077 - BBR_RS12535 0.83 -3.5 569,077 - BBR_RS12535 0.83 -2.2 569,113 + BBR_RS12535 0.86 +0.2 569,130 + BBR_RS12535 0.87 -2.3 569,130 + BBR_RS12535 0.87 -2.2 569,143 + BBR_RS12535 0.88 -3.6 569,151 - BBR_RS12535 0.89 -2.2 569,189 + -3.6 569,189 - -3.6 569,189 - -4.1 569,228 - -0.8 569,272 - -0.5 569,277 + +0.8 569,279 - -1.8 569,279 - -0.9 569,285 - -2.7 569,313 + -0.2 569,343 + -3.1 569,353 - +0.8 569,353 - -0.7 569,353 - -1.2 569,392 - +0.0 569,406 - +1.4 569,411 - -0.5 569,463 + +0.3 569,463 + -0.9 569,463 + +0.8 569,463 + -0.2 569,463 - -0.5 569,471 - -1.4 569,471 - -2.2 569,481 - +0.1 569,505 - BBR_RS12540 0.10 +0.6 569,541 + BBR_RS12540 0.13 -0.1 569,571 - BBR_RS12540 0.15 -1.0 569,606 + BBR_RS12540 0.17 +0.7 569,608 + BBR_RS12540 0.17 -2.4 569,645 - BBR_RS12540 0.19 -0.2 569,645 - BBR_RS12540 0.19 -1.2 569,775 + BBR_RS12540 0.27 -0.9 569,775 - BBR_RS12540 0.27 -0.8 569,868 + BBR_RS12540 0.33 -3.3 569,876 - BBR_RS12540 0.34 -0.4 569,897 - BBR_RS12540 0.35 +0.3 569,955 + BBR_RS12540 0.39 +1.6 569,955 + BBR_RS12540 0.39 -1.0 570,003 + BBR_RS12540 0.42 -1.5 570,013 - BBR_RS12540 0.42 +0.3 570,013 - BBR_RS12540 0.42 +0.8 570,035 - BBR_RS12540 0.44 +0.8 570,035 - BBR_RS12540 0.44 -0.7 570,042 + BBR_RS12540 0.44 -0.3 570,047 + BBR_RS12540 0.44 -0.4 570,055 - BBR_RS12540 0.45 -1.6 570,059 + BBR_RS12540 0.45 -0.0 570,108 - BBR_RS12540 0.48 +0.2 570,143 + BBR_RS12540 0.50 -1.9 570,143 + BBR_RS12540 0.50 +0.5 570,151 - BBR_RS12540 0.51 -0.9 570,151 - BBR_RS12540 0.51 +1.4 570,162 - BBR_RS12540 0.52 +0.9 570,162 - BBR_RS12540 0.52 -1.2 570,168 + BBR_RS12540 0.52 -1.6 570,176 - BBR_RS12540 0.52 +1.9 570,176 - BBR_RS12540 0.52 -0.3 570,176 - BBR_RS12540 0.52 +0.1 570,176 - BBR_RS12540 0.52 +0.5 570,176 - BBR_RS12540 0.52 -2.6 570,217 + BBR_RS12540 0.55 -0.6 570,260 - BBR_RS12540 0.58 -1.1 570,260 - BBR_RS12540 0.58 +0.3 570,260 - BBR_RS12540 0.58 +0.2 570,260 - BBR_RS12540 0.58 -0.5
Or see this region's nucleotide sequence