Experiment: LB, 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30000 and BPHYT_RS30005 are separated by 89 nucleotides BPHYT_RS30005 and BPHYT_RS30010 are separated by 80 nucleotides BPHYT_RS30010 and BPHYT_RS30015 are separated by 531 nucleotides
BPHYT_RS30000: BPHYT_RS30000 - hypothetical protein, at 2,285,690 to 2,286,484
_RS30000
BPHYT_RS30005: BPHYT_RS30005 - hypothetical protein, at 2,286,574 to 2,286,834
_RS30005
BPHYT_RS30010: BPHYT_RS30010 - cell division protein, at 2,286,915 to 2,288,819
_RS30010
BPHYT_RS30015: BPHYT_RS30015 - 2-nitropropane dioxygenase, at 2,289,351 to 2,290,376
_RS30015
Position (kb)
2286
2287
2288
2289 Strain fitness (log2 ratio)
-1
0
1 at 2286.139 kb on - strand, within BPHYT_RS30000 at 2286.139 kb on - strand, within BPHYT_RS30000 at 2286.203 kb on + strand, within BPHYT_RS30000 at 2286.204 kb on - strand, within BPHYT_RS30000 at 2287.045 kb on + strand at 2288.522 kb on - strand, within BPHYT_RS30010 at 2288.858 kb on - strand at 2289.019 kb on + strand at 2289.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 37C remove 2,286,139 - BPHYT_RS30000 0.56 +0.0 2,286,139 - BPHYT_RS30000 0.56 -1.3 2,286,203 + BPHYT_RS30000 0.65 -1.0 2,286,204 - BPHYT_RS30000 0.65 -0.3 2,287,045 + -0.6 2,288,522 - BPHYT_RS30010 0.84 -0.4 2,288,858 - +0.3 2,289,019 + -0.6 2,289,260 - -0.1
Or see this region's nucleotide sequence