Experiment: LB, 37C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21455 and BPHYT_RS21460 are separated by 66 nucleotides BPHYT_RS21460 and BPHYT_RS21465 are separated by 21 nucleotides
BPHYT_RS21455: BPHYT_RS21455 - type 11 methyltransferase, at 373,209 to 374,396
_RS21455
BPHYT_RS21460: BPHYT_RS21460 - UDP-N-acetylglucosamine 2-epimerase, at 374,463 to 375,566
_RS21460
BPHYT_RS21465: BPHYT_RS21465 - TetR family transcriptional regulator, at 375,588 to 376,316
_RS21465
Position (kb)
374
375
376 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 373.789 kb on + strand, within BPHYT_RS21455 at 373.801 kb on - strand, within BPHYT_RS21455 at 374.006 kb on + strand, within BPHYT_RS21455 at 374.213 kb on + strand, within BPHYT_RS21455 at 374.213 kb on + strand, within BPHYT_RS21455 at 374.438 kb on - strand at 375.190 kb on + strand, within BPHYT_RS21460 at 375.255 kb on + strand, within BPHYT_RS21460 at 375.474 kb on + strand at 375.536 kb on - strand at 375.763 kb on + strand, within BPHYT_RS21465 at 375.784 kb on + strand, within BPHYT_RS21465 at 375.992 kb on - strand, within BPHYT_RS21465 at 376.087 kb on + strand, within BPHYT_RS21465 at 376.141 kb on + strand, within BPHYT_RS21465 at 376.178 kb on + strand, within BPHYT_RS21465 at 376.201 kb on + strand, within BPHYT_RS21465 at 376.449 kb on - strand at 376.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 37C remove 373,789 + BPHYT_RS21455 0.49 +0.1 373,801 - BPHYT_RS21455 0.50 -0.6 374,006 + BPHYT_RS21455 0.67 -0.3 374,213 + BPHYT_RS21455 0.85 -0.6 374,213 + BPHYT_RS21455 0.85 -1.1 374,438 - -0.3 375,190 + BPHYT_RS21460 0.66 -4.1 375,255 + BPHYT_RS21460 0.72 -0.9 375,474 + -1.4 375,536 - -0.1 375,763 + BPHYT_RS21465 0.24 +1.3 375,784 + BPHYT_RS21465 0.27 +1.0 375,992 - BPHYT_RS21465 0.55 -1.6 376,087 + BPHYT_RS21465 0.68 +2.9 376,141 + BPHYT_RS21465 0.76 -0.4 376,178 + BPHYT_RS21465 0.81 -0.9 376,201 + BPHYT_RS21465 0.84 -0.5 376,449 - -2.4 376,465 - +1.6
Or see this region's nucleotide sequence