Experiment: CDM1 without Glutamate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS17680 and BBR_RS17685 are separated by 156 nucleotides BBR_RS17685 and BBR_RS17690 are separated by 459 nucleotides BBR_RS17690 and BBR_RS17695 are separated by 56 nucleotides
BBR_RS17680: BBR_RS17680 - hypothetical protein, at 1,747,720 to 1,747,947
_RS17680
BBR_RS17685: BBR_RS17685 - hypothetical protein, at 1,748,104 to 1,748,337
_RS17685
BBR_RS17690: BBR_RS17690 - CRISPR-associated endonuclease Cas2, at 1,748,797 to 1,749,087
_RS17690
BBR_RS17695: BBR_RS17695 - type I-C CRISPR-associated endonuclease Cas1, at 1,749,144 to 1,750,175
_RS17695
Position (kb)
1748
1749
1750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1747.838 kb on + strand, within BBR_RS17680 at 1747.840 kb on - strand, within BBR_RS17680 at 1747.841 kb on + strand, within BBR_RS17680 at 1747.883 kb on + strand, within BBR_RS17680 at 1747.883 kb on + strand, within BBR_RS17680 at 1747.955 kb on + strand at 1747.955 kb on + strand at 1747.968 kb on - strand at 1747.993 kb on - strand at 1748.014 kb on - strand at 1748.027 kb on + strand at 1748.027 kb on + strand at 1748.056 kb on + strand at 1748.065 kb on + strand at 1748.065 kb on - strand at 1748.072 kb on + strand at 1748.100 kb on - strand at 1748.121 kb on - strand at 1748.121 kb on - strand at 1748.121 kb on - strand at 1748.160 kb on + strand, within BBR_RS17685 at 1748.203 kb on - strand, within BBR_RS17685 at 1748.203 kb on - strand, within BBR_RS17685 at 1748.209 kb on - strand, within BBR_RS17685 at 1748.228 kb on + strand, within BBR_RS17685 at 1748.249 kb on + strand, within BBR_RS17685 at 1748.256 kb on - strand, within BBR_RS17685 at 1748.271 kb on - strand, within BBR_RS17685 at 1748.340 kb on - strand at 1748.347 kb on - strand at 1748.382 kb on + strand at 1748.394 kb on + strand at 1748.402 kb on - strand at 1748.402 kb on - strand at 1748.412 kb on - strand at 1748.422 kb on + strand at 1748.432 kb on + strand at 1748.435 kb on - strand at 1748.435 kb on - strand at 1748.440 kb on - strand at 1748.471 kb on - strand at 1748.471 kb on - strand at 1748.513 kb on - strand at 1748.542 kb on - strand at 1748.616 kb on + strand at 1748.623 kb on - strand at 1748.626 kb on + strand at 1748.634 kb on - strand at 1748.634 kb on - strand at 1748.634 kb on - strand at 1748.676 kb on + strand at 1748.701 kb on - strand at 1748.710 kb on + strand at 1748.710 kb on + strand at 1748.711 kb on - strand at 1748.752 kb on - strand at 1748.813 kb on - strand at 1748.826 kb on + strand, within BBR_RS17690 at 1748.827 kb on + strand, within BBR_RS17690 at 1748.835 kb on + strand, within BBR_RS17690 at 1748.835 kb on - strand, within BBR_RS17690 at 1748.886 kb on + strand, within BBR_RS17690 at 1748.894 kb on + strand, within BBR_RS17690 at 1748.910 kb on - strand, within BBR_RS17690 at 1748.955 kb on + strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1748.963 kb on - strand, within BBR_RS17690 at 1749.018 kb on - strand, within BBR_RS17690 at 1749.018 kb on - strand, within BBR_RS17690 at 1749.042 kb on - strand, within BBR_RS17690 at 1749.043 kb on - strand, within BBR_RS17690 at 1749.064 kb on - strand at 1749.064 kb on - strand at 1749.065 kb on + strand at 1749.087 kb on + strand at 1749.129 kb on - strand at 1749.201 kb on - strand at 1749.217 kb on + strand at 1749.227 kb on - strand at 1749.235 kb on - strand at 1749.313 kb on - strand, within BBR_RS17695 at 1749.330 kb on - strand, within BBR_RS17695 at 1749.364 kb on + strand, within BBR_RS17695 at 1749.372 kb on - strand, within BBR_RS17695 at 1749.379 kb on + strand, within BBR_RS17695 at 1749.382 kb on - strand, within BBR_RS17695 at 1749.382 kb on - strand, within BBR_RS17695 at 1749.387 kb on - strand, within BBR_RS17695 at 1749.472 kb on - strand, within BBR_RS17695 at 1749.510 kb on + strand, within BBR_RS17695 at 1749.516 kb on - strand, within BBR_RS17695 at 1749.518 kb on - strand, within BBR_RS17695 at 1749.566 kb on + strand, within BBR_RS17695 at 1749.574 kb on - strand, within BBR_RS17695 at 1749.576 kb on + strand, within BBR_RS17695 at 1749.584 kb on - strand, within BBR_RS17695 at 1749.584 kb on - strand, within BBR_RS17695 at 1749.584 kb on - strand, within BBR_RS17695 at 1749.584 kb on - strand, within BBR_RS17695 at 1749.584 kb on - strand, within BBR_RS17695 at 1749.595 kb on - strand, within BBR_RS17695 at 1749.669 kb on + strand, within BBR_RS17695 at 1749.675 kb on + strand, within BBR_RS17695 at 1749.685 kb on - strand, within BBR_RS17695 at 1749.685 kb on - strand, within BBR_RS17695 at 1749.695 kb on + strand, within BBR_RS17695 at 1749.737 kb on - strand, within BBR_RS17695 at 1749.760 kb on - strand, within BBR_RS17695 at 1749.777 kb on - strand, within BBR_RS17695 at 1749.789 kb on + strand, within BBR_RS17695 at 1749.789 kb on + strand, within BBR_RS17695 at 1749.793 kb on + strand, within BBR_RS17695 at 1749.801 kb on - strand, within BBR_RS17695 at 1749.822 kb on + strand, within BBR_RS17695 at 1749.830 kb on - strand, within BBR_RS17695 at 1749.873 kb on - strand, within BBR_RS17695 at 1749.879 kb on - strand, within BBR_RS17695 at 1749.914 kb on + strand, within BBR_RS17695 at 1749.935 kb on + strand, within BBR_RS17695 at 1749.943 kb on - strand, within BBR_RS17695 at 1749.946 kb on - strand, within BBR_RS17695 at 1750.055 kb on - strand, within BBR_RS17695
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glutamate remove 1,747,838 + BBR_RS17680 0.52 -0.4 1,747,840 - BBR_RS17680 0.53 +0.6 1,747,841 + BBR_RS17680 0.53 +0.3 1,747,883 + BBR_RS17680 0.71 -0.5 1,747,883 + BBR_RS17680 0.71 +0.1 1,747,955 + -0.9 1,747,955 + -0.3 1,747,968 - -0.6 1,747,993 - +0.2 1,748,014 - -0.6 1,748,027 + -2.1 1,748,027 + +0.2 1,748,056 + -3.7 1,748,065 + -0.1 1,748,065 - +0.7 1,748,072 + -0.2 1,748,100 - +0.5 1,748,121 - +0.2 1,748,121 - -1.6 1,748,121 - +0.6 1,748,160 + BBR_RS17685 0.24 -0.7 1,748,203 - BBR_RS17685 0.42 +0.6 1,748,203 - BBR_RS17685 0.42 +1.5 1,748,209 - BBR_RS17685 0.45 -0.5 1,748,228 + BBR_RS17685 0.53 -0.5 1,748,249 + BBR_RS17685 0.62 -1.0 1,748,256 - BBR_RS17685 0.65 +0.9 1,748,271 - BBR_RS17685 0.71 +0.5 1,748,340 - -0.2 1,748,347 - -0.7 1,748,382 + -0.5 1,748,394 + +1.6 1,748,402 - +0.4 1,748,402 - +0.0 1,748,412 - +3.1 1,748,422 + +0.1 1,748,432 + -1.4 1,748,435 - -0.5 1,748,435 - -0.7 1,748,440 - -0.4 1,748,471 - +0.1 1,748,471 - +0.3 1,748,513 - +1.1 1,748,542 - +0.1 1,748,616 + +1.2 1,748,623 - -1.7 1,748,626 + +0.1 1,748,634 - +0.0 1,748,634 - -1.2 1,748,634 - +0.1 1,748,676 + -0.3 1,748,701 - +0.8 1,748,710 + -0.8 1,748,710 + -1.0 1,748,711 - +0.5 1,748,752 - -0.6 1,748,813 - +1.1 1,748,826 + BBR_RS17690 0.10 -0.6 1,748,827 + BBR_RS17690 0.10 +0.3 1,748,835 + BBR_RS17690 0.13 -0.9 1,748,835 - BBR_RS17690 0.13 -1.2 1,748,886 + BBR_RS17690 0.31 +1.1 1,748,894 + BBR_RS17690 0.33 -3.1 1,748,910 - BBR_RS17690 0.39 -0.5 1,748,955 + BBR_RS17690 0.54 -1.8 1,748,963 - BBR_RS17690 0.57 +0.4 1,748,963 - BBR_RS17690 0.57 -0.4 1,748,963 - BBR_RS17690 0.57 -1.2 1,749,018 - BBR_RS17690 0.76 -0.8 1,749,018 - BBR_RS17690 0.76 -0.2 1,749,042 - BBR_RS17690 0.84 -0.4 1,749,043 - BBR_RS17690 0.85 -1.4 1,749,064 - -2.1 1,749,064 - +2.5 1,749,065 + +0.0 1,749,087 + -0.5 1,749,129 - +0.1 1,749,201 - -1.2 1,749,217 + -1.1 1,749,227 - -1.9 1,749,235 - -0.8 1,749,313 - BBR_RS17695 0.16 -0.5 1,749,330 - BBR_RS17695 0.18 +1.0 1,749,364 + BBR_RS17695 0.21 -0.2 1,749,372 - BBR_RS17695 0.22 -1.8 1,749,379 + BBR_RS17695 0.23 -0.3 1,749,382 - BBR_RS17695 0.23 -0.1 1,749,382 - BBR_RS17695 0.23 -0.3 1,749,387 - BBR_RS17695 0.24 +0.1 1,749,472 - BBR_RS17695 0.32 -0.7 1,749,510 + BBR_RS17695 0.35 +0.2 1,749,516 - BBR_RS17695 0.36 -1.8 1,749,518 - BBR_RS17695 0.36 -0.3 1,749,566 + BBR_RS17695 0.41 +0.2 1,749,574 - BBR_RS17695 0.42 -1.3 1,749,576 + BBR_RS17695 0.42 -0.0 1,749,584 - BBR_RS17695 0.43 +0.4 1,749,584 - BBR_RS17695 0.43 -1.2 1,749,584 - BBR_RS17695 0.43 -1.2 1,749,584 - BBR_RS17695 0.43 +0.6 1,749,584 - BBR_RS17695 0.43 +1.0 1,749,595 - BBR_RS17695 0.44 -0.8 1,749,669 + BBR_RS17695 0.51 -1.4 1,749,675 + BBR_RS17695 0.51 +0.6 1,749,685 - BBR_RS17695 0.52 -0.6 1,749,685 - BBR_RS17695 0.52 -0.1 1,749,695 + BBR_RS17695 0.53 -2.5 1,749,737 - BBR_RS17695 0.57 -2.8 1,749,760 - BBR_RS17695 0.60 +0.3 1,749,777 - BBR_RS17695 0.61 -0.8 1,749,789 + BBR_RS17695 0.62 -1.4 1,749,789 + BBR_RS17695 0.62 -0.1 1,749,793 + BBR_RS17695 0.63 +0.1 1,749,801 - BBR_RS17695 0.64 +0.6 1,749,822 + BBR_RS17695 0.66 -1.5 1,749,830 - BBR_RS17695 0.66 -1.4 1,749,873 - BBR_RS17695 0.71 -0.8 1,749,879 - BBR_RS17695 0.71 -0.5 1,749,914 + BBR_RS17695 0.75 -1.2 1,749,935 + BBR_RS17695 0.77 -0.6 1,749,943 - BBR_RS17695 0.77 +0.0 1,749,946 - BBR_RS17695 0.78 +0.8 1,750,055 - BBR_RS17695 0.88 -0.2
Or see this region's nucleotide sequence