Experiment: CDM1 without Glutamate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12365 and BBR_RS12370 are separated by 115 nucleotides BBR_RS12370 and BBR_RS12375 are separated by 5 nucleotides BBR_RS12375 and BBR_RS12380 are separated by 104 nucleotides BBR_RS12380 and BBR_RS12385 are separated by 195 nucleotides
BBR_RS12365: BBR_RS12365 - DUF177 domain-containing protein, at 525,358 to 525,978
_RS12365
BBR_RS12370: BBR_RS12370 - cell division protein, at 526,094 to 526,879
_RS12370
BBR_RS12375: BBR_RS12375 - pantetheine-phosphate adenylyltransferase, at 526,885 to 527,385
_RS12375
BBR_RS12380: BBR_RS12380 - DUF3039 domain-containing protein, at 527,490 to 527,771
_RS12380
BBR_RS12385: BBR_RS12385 - oxidoreductase, at 527,967 to 528,977
_RS12385
Position (kb)
526
527
528 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 525.890 kb on - strand, within BBR_RS12365 at 525.896 kb on + strand, within BBR_RS12365 at 525.905 kb on + strand, within BBR_RS12365 at 525.939 kb on - strand at 526.027 kb on - strand at 526.034 kb on + strand at 526.065 kb on + strand at 526.070 kb on + strand at 526.073 kb on - strand at 526.073 kb on - strand at 526.078 kb on - strand at 526.078 kb on - strand at 526.082 kb on + strand at 526.082 kb on + strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.090 kb on - strand at 526.110 kb on - strand at 526.131 kb on + strand at 526.733 kb on - strand, within BBR_RS12370 at 526.891 kb on - strand at 526.896 kb on - strand at 526.898 kb on + strand at 526.916 kb on + strand at 527.083 kb on - strand, within BBR_RS12375 at 527.125 kb on - strand, within BBR_RS12375 at 527.212 kb on - strand, within BBR_RS12375 at 527.388 kb on + strand at 527.388 kb on - strand at 527.420 kb on - strand at 527.420 kb on - strand at 527.420 kb on - strand at 527.422 kb on + strand at 527.422 kb on + strand at 527.422 kb on + strand at 527.430 kb on - strand at 527.506 kb on + strand at 527.514 kb on + strand at 527.540 kb on + strand, within BBR_RS12380 at 527.558 kb on - strand, within BBR_RS12380 at 527.561 kb on + strand, within BBR_RS12380 at 527.574 kb on - strand, within BBR_RS12380 at 527.629 kb on - strand, within BBR_RS12380 at 527.636 kb on + strand, within BBR_RS12380 at 527.666 kb on + strand, within BBR_RS12380 at 527.671 kb on + strand, within BBR_RS12380 at 527.685 kb on + strand, within BBR_RS12380 at 527.698 kb on - strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.704 kb on + strand, within BBR_RS12380 at 527.712 kb on + strand, within BBR_RS12380 at 527.712 kb on - strand, within BBR_RS12380 at 527.734 kb on - strand, within BBR_RS12380 at 527.835 kb on - strand at 527.886 kb on - strand at 527.897 kb on + strand at 527.913 kb on + strand at 527.915 kb on + strand at 527.916 kb on - strand at 528.000 kb on - strand at 528.022 kb on - strand at 528.039 kb on - strand at 528.067 kb on + strand at 528.082 kb on + strand, within BBR_RS12385 at 528.082 kb on + strand, within BBR_RS12385 at 528.090 kb on - strand, within BBR_RS12385 at 528.090 kb on - strand, within BBR_RS12385 at 528.104 kb on - strand, within BBR_RS12385 at 528.107 kb on - strand, within BBR_RS12385 at 528.114 kb on - strand, within BBR_RS12385 at 528.139 kb on - strand, within BBR_RS12385 at 528.140 kb on - strand, within BBR_RS12385 at 528.142 kb on + strand, within BBR_RS12385 at 528.150 kb on - strand, within BBR_RS12385 at 528.173 kb on - strand, within BBR_RS12385 at 528.180 kb on - strand, within BBR_RS12385 at 528.180 kb on - strand, within BBR_RS12385 at 528.252 kb on + strand, within BBR_RS12385 at 528.280 kb on + strand, within BBR_RS12385 at 528.310 kb on + strand, within BBR_RS12385 at 528.320 kb on + strand, within BBR_RS12385 at 528.328 kb on - strand, within BBR_RS12385 at 528.328 kb on - strand, within BBR_RS12385 at 528.331 kb on + strand, within BBR_RS12385 at 528.343 kb on - strand, within BBR_RS12385 at 528.348 kb on + strand, within BBR_RS12385 at 528.363 kb on + strand, within BBR_RS12385 at 528.372 kb on + strand, within BBR_RS12385 at 528.381 kb on + strand, within BBR_RS12385
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Glutamate remove 525,890 - BBR_RS12365 0.86 +0.5 525,896 + BBR_RS12365 0.87 -1.9 525,905 + BBR_RS12365 0.88 -0.6 525,939 - +0.6 526,027 - +1.7 526,034 + -0.1 526,065 + -0.4 526,070 + -0.5 526,073 - +0.2 526,073 - +1.1 526,078 - -1.0 526,078 - +1.1 526,082 + -0.4 526,082 + +1.2 526,090 - +0.4 526,090 - +0.6 526,090 - +0.5 526,110 - +0.0 526,131 + -0.3 526,733 - BBR_RS12370 0.81 +0.3 526,891 - +0.9 526,896 - -1.0 526,898 + +0.6 526,916 + +0.3 527,083 - BBR_RS12375 0.40 -1.5 527,125 - BBR_RS12375 0.48 +0.2 527,212 - BBR_RS12375 0.65 +1.3 527,388 + -1.3 527,388 - -1.5 527,420 - -0.3 527,420 - -0.3 527,420 - -2.2 527,422 + -1.5 527,422 + -0.4 527,422 + -1.4 527,430 - -0.4 527,506 + -3.1 527,514 + -2.5 527,540 + BBR_RS12380 0.18 -0.5 527,558 - BBR_RS12380 0.24 +0.2 527,561 + BBR_RS12380 0.25 -0.5 527,574 - BBR_RS12380 0.30 -1.1 527,629 - BBR_RS12380 0.49 +0.1 527,636 + BBR_RS12380 0.52 -0.3 527,666 + BBR_RS12380 0.62 +2.0 527,671 + BBR_RS12380 0.64 -3.4 527,685 + BBR_RS12380 0.69 -0.7 527,698 - BBR_RS12380 0.74 -0.5 527,704 + BBR_RS12380 0.76 -0.2 527,704 + BBR_RS12380 0.76 -0.3 527,704 + BBR_RS12380 0.76 -2.7 527,712 + BBR_RS12380 0.79 -0.6 527,712 - BBR_RS12380 0.79 -1.7 527,734 - BBR_RS12380 0.87 -1.3 527,835 - +0.5 527,886 - -0.4 527,897 + +0.3 527,913 + +2.0 527,915 + +0.6 527,916 - -0.1 528,000 - +1.4 528,022 - +1.1 528,039 - +0.1 528,067 + -1.1 528,082 + BBR_RS12385 0.11 -1.1 528,082 + BBR_RS12385 0.11 +1.4 528,090 - BBR_RS12385 0.12 -0.6 528,090 - BBR_RS12385 0.12 +0.4 528,104 - BBR_RS12385 0.14 -0.1 528,107 - BBR_RS12385 0.14 -0.0 528,114 - BBR_RS12385 0.15 +0.4 528,139 - BBR_RS12385 0.17 +0.3 528,140 - BBR_RS12385 0.17 +0.2 528,142 + BBR_RS12385 0.17 +0.9 528,150 - BBR_RS12385 0.18 -0.3 528,173 - BBR_RS12385 0.20 +0.6 528,180 - BBR_RS12385 0.21 +1.5 528,180 - BBR_RS12385 0.21 +2.4 528,252 + BBR_RS12385 0.28 -0.5 528,280 + BBR_RS12385 0.31 -0.2 528,310 + BBR_RS12385 0.34 +0.3 528,320 + BBR_RS12385 0.35 +0.5 528,328 - BBR_RS12385 0.36 +1.5 528,328 - BBR_RS12385 0.36 -0.2 528,331 + BBR_RS12385 0.36 -0.3 528,343 - BBR_RS12385 0.37 +0.0 528,348 + BBR_RS12385 0.38 -0.9 528,363 + BBR_RS12385 0.39 -0.8 528,372 + BBR_RS12385 0.40 +0.3 528,381 + BBR_RS12385 0.41 +0.7
Or see this region's nucleotide sequence