Experiment: LB, 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20449 and SM_b20450 overlap by 4 nucleotides SM_b20450 and SM_b20451 are separated by 4 nucleotides SM_b20451 and SM_b20452 are separated by 14 nucleotides SM_b20452 and SM_b20453 are separated by 35 nucleotides
SM_b20449: SM_b20449 - methyltransferase, at 461,270 to 462,328
_b20449
SM_b20450: SM_b20450 - regulatory protein, at 462,325 to 464,685
_b20450
SM_b20451: SM_b20451 - hypothetical protein, at 464,690 to 465,253
_b20451
SM_b20452: SM_b20452 - hypothetical protein, at 465,268 to 465,606
_b20452
SM_b20453: SM_b20453 - gluconolactonase, at 465,642 to 466,553
_b20453
Position (kb)
462
463
464
465 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 461.351 kb on + strand at 461.351 kb on + strand at 461.352 kb on - strand at 461.498 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.646 kb on + strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.647 kb on - strand, within SM_b20449 at 461.786 kb on + strand, within SM_b20449 at 461.786 kb on + strand, within SM_b20449 at 461.786 kb on + strand, within SM_b20449 at 461.786 kb on + strand, within SM_b20449 at 461.786 kb on + strand, within SM_b20449 at 461.788 kb on + strand, within SM_b20449 at 461.788 kb on + strand, within SM_b20449 at 461.788 kb on + strand, within SM_b20449 at 461.788 kb on + strand, within SM_b20449 at 461.788 kb on + strand, within SM_b20449 at 461.789 kb on - strand, within SM_b20449 at 461.789 kb on - strand, within SM_b20449 at 461.789 kb on - strand, within SM_b20449 at 461.789 kb on - strand, within SM_b20449 at 461.792 kb on + strand, within SM_b20449 at 461.792 kb on + strand, within SM_b20449 at 461.792 kb on + strand, within SM_b20449 at 461.793 kb on - strand, within SM_b20449 at 461.793 kb on - strand, within SM_b20449 at 461.793 kb on - strand, within SM_b20449 at 461.931 kb on - strand, within SM_b20449 at 461.932 kb on + strand, within SM_b20449 at 461.932 kb on + strand, within SM_b20449 at 461.932 kb on + strand, within SM_b20449 at 461.932 kb on + strand, within SM_b20449 at 461.932 kb on + strand, within SM_b20449 at 461.933 kb on - strand, within SM_b20449 at 461.933 kb on - strand, within SM_b20449 at 461.933 kb on - strand, within SM_b20449 at 461.933 kb on - strand, within SM_b20449 at 461.934 kb on + strand, within SM_b20449 at 461.934 kb on + strand, within SM_b20449 at 461.935 kb on - strand, within SM_b20449 at 461.996 kb on + strand, within SM_b20449 at 461.997 kb on - strand, within SM_b20449 at 461.997 kb on - strand, within SM_b20449 at 462.176 kb on + strand, within SM_b20449 at 462.177 kb on - strand, within SM_b20449 at 462.195 kb on + strand, within SM_b20449 at 462.282 kb on - strand at 462.518 kb on - strand at 462.518 kb on - strand at 462.518 kb on - strand at 462.519 kb on + strand at 462.530 kb on - strand at 462.530 kb on - strand at 462.880 kb on + strand, within SM_b20450 at 462.880 kb on + strand, within SM_b20450 at 462.881 kb on - strand, within SM_b20450 at 462.881 kb on - strand, within SM_b20450 at 462.881 kb on - strand, within SM_b20450 at 462.881 kb on - strand, within SM_b20450 at 462.881 kb on - strand, within SM_b20450 at 462.883 kb on + strand, within SM_b20450 at 462.883 kb on + strand, within SM_b20450 at 462.883 kb on + strand, within SM_b20450 at 462.883 kb on + strand, within SM_b20450 at 462.885 kb on + strand, within SM_b20450 at 462.886 kb on - strand, within SM_b20450 at 462.926 kb on - strand, within SM_b20450 at 462.926 kb on - strand, within SM_b20450 at 462.928 kb on - strand, within SM_b20450 at 463.009 kb on + strand, within SM_b20450 at 463.085 kb on - strand, within SM_b20450 at 463.085 kb on - strand, within SM_b20450 at 463.085 kb on - strand, within SM_b20450 at 463.086 kb on + strand, within SM_b20450 at 463.218 kb on + strand, within SM_b20450 at 463.459 kb on + strand, within SM_b20450 at 463.624 kb on + strand, within SM_b20450 at 463.624 kb on + strand, within SM_b20450 at 463.706 kb on + strand, within SM_b20450 at 463.816 kb on - strand, within SM_b20450 at 463.975 kb on - strand, within SM_b20450 at 463.975 kb on - strand, within SM_b20450 at 464.041 kb on + strand, within SM_b20450 at 464.041 kb on + strand, within SM_b20450 at 464.042 kb on - strand, within SM_b20450 at 464.043 kb on + strand, within SM_b20450 at 464.043 kb on + strand, within SM_b20450 at 464.043 kb on + strand, within SM_b20450 at 464.075 kb on - strand, within SM_b20450 at 464.075 kb on - strand, within SM_b20450 at 464.077 kb on + strand, within SM_b20450 at 464.077 kb on + strand, within SM_b20450 at 464.177 kb on + strand, within SM_b20450 at 464.249 kb on - strand, within SM_b20450 at 464.249 kb on - strand, within SM_b20450 at 464.490 kb on + strand at 464.700 kb on + strand at 464.700 kb on + strand at 464.700 kb on + strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.701 kb on - strand at 464.702 kb on + strand at 464.702 kb on + strand at 464.702 kb on + strand at 464.703 kb on - strand at 464.703 kb on - strand at 464.703 kb on - strand at 464.886 kb on - strand, within SM_b20451 at 465.042 kb on + strand, within SM_b20451 at 465.043 kb on - strand, within SM_b20451 at 465.043 kb on - strand, within SM_b20451 at 465.046 kb on - strand, within SM_b20451 at 465.049 kb on - strand, within SM_b20451 at 465.082 kb on - strand, within SM_b20451 at 465.152 kb on + strand, within SM_b20451 at 465.153 kb on - strand, within SM_b20451 at 465.218 kb on + strand at 465.218 kb on + strand at 465.218 kb on + strand at 465.218 kb on + strand at 465.218 kb on + strand at 465.219 kb on - strand at 465.219 kb on - strand at 465.219 kb on - strand at 465.227 kb on - strand at 465.319 kb on + strand, within SM_b20452 at 465.319 kb on + strand, within SM_b20452 at 465.319 kb on + strand, within SM_b20452 at 465.320 kb on - strand, within SM_b20452 at 465.388 kb on - strand, within SM_b20452 at 465.388 kb on - strand, within SM_b20452 at 465.513 kb on + strand, within SM_b20452 at 465.599 kb on + strand at 465.644 kb on - strand at 465.673 kb on + strand at 465.674 kb on - strand at 465.674 kb on - strand at 465.682 kb on + strand at 465.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 23C remove 461,351 + +0.0 461,351 + -1.5 461,352 - +2.3 461,498 + SM_b20449 0.22 -0.8 461,646 + SM_b20449 0.36 -1.0 461,646 + SM_b20449 0.36 -0.3 461,646 + SM_b20449 0.36 +0.8 461,646 + SM_b20449 0.36 -1.0 461,646 + SM_b20449 0.36 +0.0 461,646 + SM_b20449 0.36 -1.0 461,646 + SM_b20449 0.36 -0.5 461,646 + SM_b20449 0.36 -1.1 461,646 + SM_b20449 0.36 -0.4 461,646 + SM_b20449 0.36 +0.0 461,646 + SM_b20449 0.36 -0.2 461,646 + SM_b20449 0.36 -0.1 461,647 - SM_b20449 0.36 -1.1 461,647 - SM_b20449 0.36 -0.4 461,647 - SM_b20449 0.36 +0.1 461,647 - SM_b20449 0.36 -0.9 461,647 - SM_b20449 0.36 -1.6 461,647 - SM_b20449 0.36 +0.3 461,647 - SM_b20449 0.36 -1.7 461,786 + SM_b20449 0.49 -1.3 461,786 + SM_b20449 0.49 +0.7 461,786 + SM_b20449 0.49 +0.4 461,786 + SM_b20449 0.49 -0.6 461,786 + SM_b20449 0.49 +0.4 461,788 + SM_b20449 0.49 -0.2 461,788 + SM_b20449 0.49 +0.2 461,788 + SM_b20449 0.49 -0.5 461,788 + SM_b20449 0.49 +1.1 461,788 + SM_b20449 0.49 +0.0 461,789 - SM_b20449 0.49 -0.6 461,789 - SM_b20449 0.49 -0.6 461,789 - SM_b20449 0.49 -0.4 461,789 - SM_b20449 0.49 -1.3 461,792 + SM_b20449 0.49 +0.1 461,792 + SM_b20449 0.49 +1.1 461,792 + SM_b20449 0.49 -0.4 461,793 - SM_b20449 0.49 -0.3 461,793 - SM_b20449 0.49 -0.6 461,793 - SM_b20449 0.49 -0.6 461,931 - SM_b20449 0.62 -0.4 461,932 + SM_b20449 0.63 +0.1 461,932 + SM_b20449 0.63 -0.9 461,932 + SM_b20449 0.63 +0.3 461,932 + SM_b20449 0.63 +0.1 461,932 + SM_b20449 0.63 -0.4 461,933 - SM_b20449 0.63 -1.1 461,933 - SM_b20449 0.63 -1.3 461,933 - SM_b20449 0.63 +0.4 461,933 - SM_b20449 0.63 -1.8 461,934 + SM_b20449 0.63 -0.3 461,934 + SM_b20449 0.63 +0.6 461,935 - SM_b20449 0.63 -1.6 461,996 + SM_b20449 0.69 +0.7 461,997 - SM_b20449 0.69 -0.3 461,997 - SM_b20449 0.69 -0.6 462,176 + SM_b20449 0.86 -0.7 462,177 - SM_b20449 0.86 -1.0 462,195 + SM_b20449 0.87 -0.6 462,282 - -0.7 462,518 - -1.6 462,518 - -1.5 462,518 - -0.9 462,519 + +0.2 462,530 - -0.9 462,530 - +0.0 462,880 + SM_b20450 0.24 -2.8 462,880 + SM_b20450 0.24 -1.9 462,881 - SM_b20450 0.24 -0.7 462,881 - SM_b20450 0.24 +0.7 462,881 - SM_b20450 0.24 -1.0 462,881 - SM_b20450 0.24 -0.9 462,881 - SM_b20450 0.24 -0.3 462,883 + SM_b20450 0.24 +0.4 462,883 + SM_b20450 0.24 +0.1 462,883 + SM_b20450 0.24 -2.2 462,883 + SM_b20450 0.24 +1.5 462,885 + SM_b20450 0.24 -1.3 462,886 - SM_b20450 0.24 -2.8 462,926 - SM_b20450 0.25 -1.1 462,926 - SM_b20450 0.25 -0.8 462,928 - SM_b20450 0.26 -1.3 463,009 + SM_b20450 0.29 -0.9 463,085 - SM_b20450 0.32 +0.2 463,085 - SM_b20450 0.32 -0.7 463,085 - SM_b20450 0.32 -1.1 463,086 + SM_b20450 0.32 +0.4 463,218 + SM_b20450 0.38 +0.3 463,459 + SM_b20450 0.48 +0.2 463,624 + SM_b20450 0.55 -0.9 463,624 + SM_b20450 0.55 -2.0 463,706 + SM_b20450 0.58 -1.1 463,816 - SM_b20450 0.63 -1.3 463,975 - SM_b20450 0.70 -0.7 463,975 - SM_b20450 0.70 -1.0 464,041 + SM_b20450 0.73 +0.5 464,041 + SM_b20450 0.73 -0.5 464,042 - SM_b20450 0.73 -0.4 464,043 + SM_b20450 0.73 -1.3 464,043 + SM_b20450 0.73 -0.6 464,043 + SM_b20450 0.73 +0.1 464,075 - SM_b20450 0.74 -0.5 464,075 - SM_b20450 0.74 -0.6 464,077 + SM_b20450 0.74 -0.4 464,077 + SM_b20450 0.74 -2.0 464,177 + SM_b20450 0.78 -0.9 464,249 - SM_b20450 0.81 -1.2 464,249 - SM_b20450 0.81 -1.4 464,490 + -0.2 464,700 + -0.0 464,700 + -2.4 464,700 + +0.2 464,701 - -0.1 464,701 - +0.9 464,701 - +0.6 464,701 - +1.7 464,701 - +0.3 464,701 - +0.5 464,701 - -0.9 464,702 + -1.2 464,702 + +1.2 464,702 + +0.6 464,703 - -0.8 464,703 - -0.2 464,703 - +0.1 464,886 - SM_b20451 0.35 -0.4 465,042 + SM_b20451 0.62 -0.7 465,043 - SM_b20451 0.63 -0.1 465,043 - SM_b20451 0.63 +0.1 465,046 - SM_b20451 0.63 +0.1 465,049 - SM_b20451 0.64 +0.2 465,082 - SM_b20451 0.70 -0.3 465,152 + SM_b20451 0.82 +0.5 465,153 - SM_b20451 0.82 -0.7 465,218 + -0.3 465,218 + +1.0 465,218 + -0.1 465,218 + +0.9 465,218 + +0.2 465,219 - -0.9 465,219 - -0.4 465,219 - +1.7 465,227 - -1.0 465,319 + SM_b20452 0.15 +0.4 465,319 + SM_b20452 0.15 -1.4 465,319 + SM_b20452 0.15 +0.2 465,320 - SM_b20452 0.15 -1.9 465,388 - SM_b20452 0.35 -0.9 465,388 - SM_b20452 0.35 -1.9 465,513 + SM_b20452 0.72 -0.2 465,599 + -0.3 465,644 - -1.0 465,673 + +0.4 465,674 - +0.3 465,674 - +0.4 465,682 + +0.3 465,683 - +0.0
Or see this region's nucleotide sequence