Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04760 and MMJJ_RS04765 are separated by 40 nucleotides MMJJ_RS04765 and MMJJ_RS04770 are separated by 18 nucleotides MMJJ_RS04770 and MMJJ_RS04775 are separated by 180 nucleotides
MMJJ_RS04760: MMJJ_RS04760 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 893,108 to 894,640
_RS04760
MMJJ_RS04765: MMJJ_RS04765 - metal-dependent hydrolase, at 894,681 to 895,355
_RS04765
MMJJ_RS04770: MMJJ_RS04770 - formate-dependent phosphoribosylglycinamide formyltransferase, at 895,374 to 896,540
_RS04770
MMJJ_RS04775: MMJJ_RS04775 - DNA-binding protein, at 896,721 to 897,632
_RS04775
Position (kb)
895
896
897 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.404 kb on + strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.496 kb on + strand at 894.511 kb on + strand at 894.518 kb on - strand at 894.519 kb on + strand at 894.519 kb on + strand at 894.575 kb on + strand at 894.577 kb on - strand at 894.578 kb on + strand at 894.578 kb on + strand at 894.601 kb on + strand at 894.634 kb on + strand at 894.634 kb on + strand at 894.634 kb on + strand at 894.698 kb on - strand at 894.699 kb on + strand at 894.699 kb on + strand at 894.732 kb on + strand at 894.732 kb on + strand at 894.761 kb on + strand, within MMJJ_RS04765 at 894.788 kb on + strand, within MMJJ_RS04765 at 894.799 kb on - strand, within MMJJ_RS04765 at 894.812 kb on + strand, within MMJJ_RS04765 at 894.812 kb on + strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.829 kb on + strand, within MMJJ_RS04765 at 894.829 kb on + strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.831 kb on + strand, within MMJJ_RS04765 at 894.831 kb on + strand, within MMJJ_RS04765 at 894.846 kb on + strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.871 kb on - strand, within MMJJ_RS04765 at 894.871 kb on - strand, within MMJJ_RS04765 at 894.885 kb on - strand, within MMJJ_RS04765 at 894.935 kb on - strand, within MMJJ_RS04765 at 894.949 kb on + strand, within MMJJ_RS04765 at 894.995 kb on - strand, within MMJJ_RS04765 at 895.088 kb on - strand, within MMJJ_RS04765 at 895.089 kb on + strand, within MMJJ_RS04765 at 895.160 kb on - strand, within MMJJ_RS04765 at 895.161 kb on + strand, within MMJJ_RS04765 at 895.185 kb on - strand, within MMJJ_RS04765 at 895.190 kb on + strand, within MMJJ_RS04765 at 895.209 kb on - strand, within MMJJ_RS04765 at 895.277 kb on - strand, within MMJJ_RS04765 at 895.287 kb on + strand, within MMJJ_RS04765 at 895.599 kb on - strand, within MMJJ_RS04770 at 895.677 kb on + strand, within MMJJ_RS04770 at 895.707 kb on - strand, within MMJJ_RS04770 at 896.085 kb on - strand, within MMJJ_RS04770 at 896.086 kb on + strand, within MMJJ_RS04770 at 896.090 kb on - strand at 896.280 kb on + strand, within MMJJ_RS04770 at 896.310 kb on - strand, within MMJJ_RS04770 at 896.558 kb on - strand at 896.598 kb on - strand at 896.610 kb on - strand at 896.610 kb on - strand at 896.610 kb on - strand at 896.610 kb on - strand at 896.611 kb on + strand at 896.613 kb on - strand at 896.613 kb on - strand at 896.613 kb on - strand at 896.614 kb on + strand at 896.614 kb on + strand at 896.615 kb on - strand at 896.615 kb on - strand at 896.615 kb on - strand at 896.616 kb on + strand at 896.616 kb on + strand at 896.616 kb on + strand at 896.638 kb on + strand at 896.686 kb on - strand at 896.686 kb on - strand at 896.706 kb on - strand at 896.750 kb on - strand at 896.751 kb on + strand at 896.751 kb on + strand at 896.751 kb on + strand at 896.752 kb on - strand at 896.752 kb on - strand at 896.752 kb on - strand at 896.781 kb on + strand at 896.810 kb on - strand at 896.812 kb on - strand at 896.812 kb on - strand at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.813 kb on + strand, within MMJJ_RS04775 at 896.816 kb on - strand, within MMJJ_RS04775 at 896.817 kb on + strand, within MMJJ_RS04775 at 896.817 kb on + strand, within MMJJ_RS04775 at 896.870 kb on - strand, within MMJJ_RS04775 at 896.910 kb on + strand, within MMJJ_RS04775 at 896.921 kb on + strand, within MMJJ_RS04775 at 896.960 kb on + strand, within MMJJ_RS04775 at 897.019 kb on - strand, within MMJJ_RS04775 at 897.067 kb on - strand, within MMJJ_RS04775 at 897.068 kb on + strand, within MMJJ_RS04775 at 897.187 kb on - strand, within MMJJ_RS04775 at 897.188 kb on + strand, within MMJJ_RS04775 at 897.364 kb on - strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.413 kb on + strand, within MMJJ_RS04775 at 897.417 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.423 kb on + strand, within MMJJ_RS04775 at 897.518 kb on + strand, within MMJJ_RS04775 at 897.520 kb on - strand, within MMJJ_RS04775 at 897.538 kb on - strand, within MMJJ_RS04775 at 897.539 kb on + strand, within MMJJ_RS04775 at 897.540 kb on - strand, within MMJJ_RS04775
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 894,401 - MMJJ_RS04760 0.84 +0.8 894,401 - MMJJ_RS04760 0.84 +0.4 894,401 - MMJJ_RS04760 0.84 +0.3 894,401 - MMJJ_RS04760 0.84 -0.8 894,401 - MMJJ_RS04760 0.84 -0.0 894,402 + MMJJ_RS04760 0.84 -0.3 894,402 + MMJJ_RS04760 0.84 +0.6 894,402 + MMJJ_RS04760 0.84 +0.2 894,402 + MMJJ_RS04760 0.84 +0.2 894,404 + MMJJ_RS04760 0.85 -0.2 894,444 - MMJJ_RS04760 0.87 +0.1 894,444 - MMJJ_RS04760 0.87 -0.4 894,444 - MMJJ_RS04760 0.87 +0.2 894,445 + MMJJ_RS04760 0.87 -0.2 894,445 + MMJJ_RS04760 0.87 -1.6 894,445 + MMJJ_RS04760 0.87 +0.1 894,445 + MMJJ_RS04760 0.87 -0.4 894,445 + MMJJ_RS04760 0.87 -0.9 894,445 + MMJJ_RS04760 0.87 +0.2 894,445 + MMJJ_RS04760 0.87 +0.3 894,445 + MMJJ_RS04760 0.87 +0.4 894,446 - MMJJ_RS04760 0.87 +0.7 894,446 - MMJJ_RS04760 0.87 +0.2 894,446 - MMJJ_RS04760 0.87 -1.7 894,446 - MMJJ_RS04760 0.87 -0.7 894,447 + MMJJ_RS04760 0.87 +0.8 894,447 + MMJJ_RS04760 0.87 -0.9 894,447 + MMJJ_RS04760 0.87 -0.1 894,447 + MMJJ_RS04760 0.87 -0.6 894,447 + MMJJ_RS04760 0.87 +0.7 894,447 + MMJJ_RS04760 0.87 -0.4 894,447 + MMJJ_RS04760 0.87 -0.6 894,450 - MMJJ_RS04760 0.88 -0.6 894,450 - MMJJ_RS04760 0.88 -0.2 894,450 - MMJJ_RS04760 0.88 -0.4 894,496 + +0.5 894,511 + -0.1 894,518 - -1.3 894,519 + +0.5 894,519 + -0.8 894,575 + +0.2 894,577 - -0.3 894,578 + -0.1 894,578 + +1.0 894,601 + -0.1 894,634 + +0.5 894,634 + +0.2 894,634 + +1.5 894,698 - -2.4 894,699 + -0.1 894,699 + +0.3 894,732 + -1.3 894,732 + +0.2 894,761 + MMJJ_RS04765 0.12 -1.8 894,788 + MMJJ_RS04765 0.16 -0.5 894,799 - MMJJ_RS04765 0.17 +0.0 894,812 + MMJJ_RS04765 0.19 -0.6 894,812 + MMJJ_RS04765 0.19 -1.4 894,828 - MMJJ_RS04765 0.22 -1.4 894,828 - MMJJ_RS04765 0.22 -0.9 894,828 - MMJJ_RS04765 0.22 -1.0 894,828 - MMJJ_RS04765 0.22 -2.2 894,828 - MMJJ_RS04765 0.22 -0.5 894,828 - MMJJ_RS04765 0.22 -1.4 894,829 + MMJJ_RS04765 0.22 +0.3 894,829 + MMJJ_RS04765 0.22 -1.2 894,830 - MMJJ_RS04765 0.22 -0.4 894,830 - MMJJ_RS04765 0.22 -0.7 894,830 - MMJJ_RS04765 0.22 -0.4 894,831 + MMJJ_RS04765 0.22 -1.3 894,831 + MMJJ_RS04765 0.22 -0.5 894,846 + MMJJ_RS04765 0.24 -0.7 894,867 - MMJJ_RS04765 0.28 -1.6 894,867 - MMJJ_RS04765 0.28 -1.6 894,867 - MMJJ_RS04765 0.28 -0.6 894,867 - MMJJ_RS04765 0.28 -1.8 894,871 - MMJJ_RS04765 0.28 -2.5 894,871 - MMJJ_RS04765 0.28 +0.5 894,885 - MMJJ_RS04765 0.30 +0.2 894,935 - MMJJ_RS04765 0.38 -2.0 894,949 + MMJJ_RS04765 0.40 -1.4 894,995 - MMJJ_RS04765 0.47 -1.2 895,088 - MMJJ_RS04765 0.60 -3.6 895,089 + MMJJ_RS04765 0.60 -0.6 895,160 - MMJJ_RS04765 0.71 -0.0 895,161 + MMJJ_RS04765 0.71 -0.9 895,185 - MMJJ_RS04765 0.75 -3.0 895,190 + MMJJ_RS04765 0.75 -0.4 895,209 - MMJJ_RS04765 0.78 -0.4 895,277 - MMJJ_RS04765 0.88 -0.5 895,287 + MMJJ_RS04765 0.90 -0.6 895,599 - MMJJ_RS04770 0.19 -0.5 895,677 + MMJJ_RS04770 0.26 -2.7 895,707 - MMJJ_RS04770 0.29 +0.2 896,085 - MMJJ_RS04770 0.61 -2.2 896,086 + MMJJ_RS04770 0.61 -1.8 896,090 - -1.6 896,280 + MMJJ_RS04770 0.78 -2.6 896,310 - MMJJ_RS04770 0.80 -0.8 896,558 - -0.8 896,598 - +0.7 896,610 - -0.6 896,610 - +0.1 896,610 - -0.6 896,610 - -0.6 896,611 + +0.1 896,613 - -0.8 896,613 - +0.4 896,613 - -0.7 896,614 + -0.5 896,614 + +0.5 896,615 - -1.2 896,615 - +0.1 896,615 - -0.8 896,616 + +0.2 896,616 + +0.9 896,616 + -0.4 896,638 + -0.3 896,686 - +1.1 896,686 - -0.4 896,706 - +0.1 896,750 - -0.3 896,751 + -0.8 896,751 + +0.3 896,751 + +0.2 896,752 - +0.8 896,752 - -0.3 896,752 - +0.2 896,781 + -0.3 896,810 - -0.4 896,812 - +0.5 896,812 - +0.1 896,813 + MMJJ_RS04775 0.10 +0.4 896,813 + MMJJ_RS04775 0.10 +0.5 896,813 + MMJJ_RS04775 0.10 -0.5 896,813 + MMJJ_RS04775 0.10 -0.5 896,813 + MMJJ_RS04775 0.10 -0.4 896,813 + MMJJ_RS04775 0.10 +0.9 896,816 - MMJJ_RS04775 0.10 +0.1 896,817 + MMJJ_RS04775 0.11 -0.9 896,817 + MMJJ_RS04775 0.11 -1.0 896,870 - MMJJ_RS04775 0.16 +0.1 896,910 + MMJJ_RS04775 0.21 -0.5 896,921 + MMJJ_RS04775 0.22 +0.2 896,960 + MMJJ_RS04775 0.26 -1.0 897,019 - MMJJ_RS04775 0.33 -0.1 897,067 - MMJJ_RS04775 0.38 +1.1 897,068 + MMJJ_RS04775 0.38 +0.4 897,187 - MMJJ_RS04775 0.51 +0.4 897,188 + MMJJ_RS04775 0.51 -0.8 897,364 - MMJJ_RS04775 0.71 +0.4 897,413 + MMJJ_RS04775 0.76 +0.3 897,413 + MMJJ_RS04775 0.76 +0.6 897,417 + MMJJ_RS04775 0.76 +0.7 897,423 + MMJJ_RS04775 0.77 +0.1 897,423 + MMJJ_RS04775 0.77 -0.0 897,518 + MMJJ_RS04775 0.87 -0.4 897,520 - MMJJ_RS04775 0.88 +0.1 897,538 - MMJJ_RS04775 0.90 +0.2 897,539 + MMJJ_RS04775 0.90 -1.3 897,540 - MMJJ_RS04775 0.90 +0.5
Or see this region's nucleotide sequence