Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00060 and MMJJ_RS00065 are separated by 4 nucleotides MMJJ_RS00065 and MMJJ_RS00070 are separated by 104 nucleotides MMJJ_RS00070 and MMJJ_RS00075 are separated by 12 nucleotides MMJJ_RS00075 and MMJJ_RS00080 overlap by 4 nucleotides MMJJ_RS00080 and MMJJ_RS00085 are separated by 218 nucleotides
MMJJ_RS00060: MMJJ_RS00060 - acetyl-CoA decarbonylase/synthase complex subunit gamma, at 12,610 to 13,983
_RS00060
MMJJ_RS00065: MMJJ_RS00065 - 4Fe-4S dicluster domain-containing protein, at 13,988 to 14,452
_RS00065
MMJJ_RS00070: MMJJ_RS00070 - CooT family nickel-binding protein, at 14,557 to 14,736
_RS00070
MMJJ_RS00075: MMJJ_RS00075 - AAA family ATPase, at 14,749 to 15,531
_RS00075
MMJJ_RS00080: MMJJ_RS00080 - DUF166 domain-containing protein, at 15,528 to 16,184
_RS00080
MMJJ_RS00085: MMJJ_RS00085 - substrate-binding domain-containing protein, at 16,403 to 17,254
_RS00085
Position (kb) 14
15
16 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 13.750 kb on + strand, within MMJJ_RS00060 at 13.818 kb on - strand, within MMJJ_RS00060 at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.925 kb on - strand at 13.926 kb on + strand at 13.926 kb on + strand at 13.968 kb on + strand at 14.005 kb on + strand at 14.041 kb on - strand, within MMJJ_RS00065 at 14.041 kb on - strand, within MMJJ_RS00065 at 14.043 kb on - strand, within MMJJ_RS00065 at 14.053 kb on - strand, within MMJJ_RS00065 at 14.054 kb on + strand, within MMJJ_RS00065 at 14.080 kb on - strand, within MMJJ_RS00065 at 14.081 kb on + strand, within MMJJ_RS00065 at 14.081 kb on + strand, within MMJJ_RS00065 at 14.083 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.140 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.148 kb on - strand, within MMJJ_RS00065 at 14.149 kb on + strand, within MMJJ_RS00065 at 14.157 kb on - strand, within MMJJ_RS00065 at 14.175 kb on - strand, within MMJJ_RS00065 at 14.176 kb on + strand, within MMJJ_RS00065 at 14.190 kb on - strand, within MMJJ_RS00065 at 14.211 kb on - strand, within MMJJ_RS00065 at 14.242 kb on - strand, within MMJJ_RS00065 at 14.254 kb on - strand at 14.290 kb on - strand, within MMJJ_RS00065 at 14.312 kb on + strand, within MMJJ_RS00065 at 14.341 kb on - strand, within MMJJ_RS00065 at 14.341 kb on - strand, within MMJJ_RS00065 at 14.361 kb on - strand, within MMJJ_RS00065 at 14.389 kb on - strand, within MMJJ_RS00065 at 14.390 kb on + strand, within MMJJ_RS00065 at 14.450 kb on - strand at 14.450 kb on - strand at 14.474 kb on - strand at 14.484 kb on - strand at 14.485 kb on + strand at 14.496 kb on - strand at 14.496 kb on - strand at 14.504 kb on - strand at 14.509 kb on - strand at 14.516 kb on - strand at 14.540 kb on - strand at 14.556 kb on - strand at 14.556 kb on - strand at 14.557 kb on + strand at 14.557 kb on + strand at 14.589 kb on - strand, within MMJJ_RS00070 at 14.594 kb on - strand, within MMJJ_RS00070 at 14.595 kb on + strand, within MMJJ_RS00070 at 14.717 kb on - strand, within MMJJ_RS00070 at 14.735 kb on + strand at 14.822 kb on + strand at 14.854 kb on - strand, within MMJJ_RS00075 at 14.855 kb on + strand, within MMJJ_RS00075 at 14.855 kb on + strand, within MMJJ_RS00075 at 14.963 kb on + strand, within MMJJ_RS00075 at 15.112 kb on - strand, within MMJJ_RS00075 at 15.112 kb on - strand, within MMJJ_RS00075 at 15.112 kb on - strand, within MMJJ_RS00075 at 15.112 kb on - strand, within MMJJ_RS00075 at 15.113 kb on + strand, within MMJJ_RS00075 at 15.113 kb on + strand, within MMJJ_RS00075 at 15.113 kb on + strand, within MMJJ_RS00075 at 15.113 kb on + strand, within MMJJ_RS00075 at 15.113 kb on + strand, within MMJJ_RS00075 at 15.118 kb on - strand, within MMJJ_RS00075 at 15.119 kb on + strand, within MMJJ_RS00075 at 15.153 kb on - strand, within MMJJ_RS00075 at 15.154 kb on + strand, within MMJJ_RS00075 at 15.154 kb on + strand, within MMJJ_RS00075 at 15.154 kb on + strand at 15.154 kb on + strand, within MMJJ_RS00075 at 15.164 kb on + strand, within MMJJ_RS00075 at 15.184 kb on - strand, within MMJJ_RS00075 at 15.184 kb on - strand, within MMJJ_RS00075 at 15.184 kb on - strand, within MMJJ_RS00075 at 15.256 kb on - strand, within MMJJ_RS00075 at 15.262 kb on - strand, within MMJJ_RS00075 at 15.295 kb on - strand, within MMJJ_RS00075 at 15.385 kb on + strand, within MMJJ_RS00075 at 15.409 kb on - strand, within MMJJ_RS00075 at 15.433 kb on - strand, within MMJJ_RS00075 at 15.434 kb on + strand, within MMJJ_RS00075 at 15.464 kb on + strand at 15.464 kb on + strand at 15.465 kb on - strand at 15.465 kb on - strand at 15.465 kb on - strand at 15.465 kb on - strand at 15.466 kb on + strand at 15.473 kb on + strand at 15.516 kb on - strand at 15.655 kb on + strand, within MMJJ_RS00080 at 15.655 kb on + strand, within MMJJ_RS00080 at 15.748 kb on + strand, within MMJJ_RS00080 at 15.748 kb on + strand, within MMJJ_RS00080 at 15.832 kb on + strand, within MMJJ_RS00080 at 15.876 kb on - strand, within MMJJ_RS00080 at 15.876 kb on - strand, within MMJJ_RS00080 at 15.876 kb on - strand, within MMJJ_RS00080 at 15.937 kb on + strand, within MMJJ_RS00080 at 15.937 kb on + strand, within MMJJ_RS00080 at 15.937 kb on + strand, within MMJJ_RS00080 at 15.937 kb on + strand, within MMJJ_RS00080 at 15.937 kb on + strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.939 kb on - strand, within MMJJ_RS00080 at 15.940 kb on + strand, within MMJJ_RS00080 at 15.940 kb on + strand, within MMJJ_RS00080 at 15.940 kb on + strand, within MMJJ_RS00080 at 15.941 kb on - strand, within MMJJ_RS00080 at 15.941 kb on - strand, within MMJJ_RS00080 at 15.941 kb on - strand, within MMJJ_RS00080 at 15.941 kb on - strand, within MMJJ_RS00080 at 15.942 kb on + strand, within MMJJ_RS00080 at 15.942 kb on + strand, within MMJJ_RS00080 at 15.942 kb on + strand, within MMJJ_RS00080 at 15.942 kb on + strand, within MMJJ_RS00080 at 15.944 kb on + strand, within MMJJ_RS00080 at 15.958 kb on - strand, within MMJJ_RS00080 at 15.997 kb on + strand, within MMJJ_RS00080 at 15.997 kb on + strand, within MMJJ_RS00080 at 15.999 kb on - strand, within MMJJ_RS00080 at 16.003 kb on - strand, within MMJJ_RS00080 at 16.003 kb on - strand, within MMJJ_RS00080 at 16.003 kb on - strand, within MMJJ_RS00080 at 16.004 kb on + strand, within MMJJ_RS00080 at 16.004 kb on + strand, within MMJJ_RS00080 at 16.074 kb on + strand, within MMJJ_RS00080 at 16.074 kb on + strand, within MMJJ_RS00080 at 16.100 kb on - strand, within MMJJ_RS00080 at 16.103 kb on - strand, within MMJJ_RS00080 at 16.103 kb on - strand, within MMJJ_RS00080 at 16.105 kb on - strand, within MMJJ_RS00080 at 16.127 kb on - strand at 16.139 kb on + strand at 16.139 kb on + strand at 16.212 kb on - strand at 16.212 kb on - strand at 16.214 kb on - strand at 16.215 kb on + strand at 16.215 kb on + strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.226 kb on - strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.227 kb on + strand at 16.228 kb on - strand at 16.228 kb on - strand at 16.229 kb on + strand at 16.229 kb on + strand at 16.230 kb on - strand at 16.230 kb on - strand at 16.235 kb on + strand at 16.266 kb on + strand at 16.307 kb on - strand at 16.307 kb on - strand at 16.307 kb on - strand at 16.307 kb on - strand at 16.308 kb on + strand at 16.308 kb on + strand at 16.308 kb on + strand at 16.336 kb on + strand at 16.349 kb on + strand at 16.400 kb on - strand at 16.401 kb on + strand at 16.401 kb on + strand at 16.415 kb on - strand at 16.468 kb on - strand at 16.469 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 13,750 + MMJJ_RS00060 0.83 -2.1 13,818 - MMJJ_RS00060 0.88 -2.4 13,925 - -2.7 13,925 - -2.9 13,925 - -2.6 13,925 - -2.1 13,925 - -3.6 13,925 - -3.0 13,926 + -2.8 13,926 + -2.8 13,968 + -2.7 14,005 + -2.3 14,041 - MMJJ_RS00065 0.11 -2.5 14,041 - MMJJ_RS00065 0.11 -2.8 14,043 - MMJJ_RS00065 0.12 -4.8 14,053 - MMJJ_RS00065 0.14 -2.4 14,054 + MMJJ_RS00065 0.14 -1.7 14,080 - MMJJ_RS00065 0.20 -1.6 14,081 + MMJJ_RS00065 0.20 -2.1 14,081 + MMJJ_RS00065 0.20 -0.8 14,083 - MMJJ_RS00065 0.20 -3.6 14,140 - MMJJ_RS00065 0.33 -3.4 14,140 - MMJJ_RS00065 0.33 -2.6 14,140 - MMJJ_RS00065 0.33 -1.0 14,140 - MMJJ_RS00065 0.33 -2.1 14,140 - MMJJ_RS00065 0.33 -2.4 14,148 - MMJJ_RS00065 0.34 -3.8 14,148 - MMJJ_RS00065 0.34 -1.6 14,148 - MMJJ_RS00065 0.34 -2.9 14,149 + MMJJ_RS00065 0.35 -4.8 14,157 - MMJJ_RS00065 0.36 -2.4 14,175 - MMJJ_RS00065 0.40 -1.3 14,176 + MMJJ_RS00065 0.40 -2.4 14,190 - MMJJ_RS00065 0.43 -1.0 14,211 - MMJJ_RS00065 0.48 -2.1 14,242 - MMJJ_RS00065 0.55 -2.8 14,254 - +0.6 14,290 - MMJJ_RS00065 0.65 -2.0 14,312 + MMJJ_RS00065 0.70 -1.5 14,341 - MMJJ_RS00065 0.76 -2.5 14,341 - MMJJ_RS00065 0.76 -2.7 14,361 - MMJJ_RS00065 0.80 -2.0 14,389 - MMJJ_RS00065 0.86 -1.8 14,390 + MMJJ_RS00065 0.86 +0.4 14,450 - -1.0 14,450 - -0.7 14,474 - -1.3 14,484 - -2.0 14,485 + -0.4 14,496 - -0.2 14,496 - -0.7 14,504 - -1.6 14,509 - -2.5 14,516 - -2.1 14,540 - -1.9 14,556 - -1.0 14,556 - -2.2 14,557 + -2.8 14,557 + -0.8 14,589 - MMJJ_RS00070 0.18 -1.4 14,594 - MMJJ_RS00070 0.21 -2.1 14,595 + MMJJ_RS00070 0.21 -1.3 14,717 - MMJJ_RS00070 0.89 -1.1 14,735 + -0.6 14,822 + -2.0 14,854 - MMJJ_RS00075 0.13 -1.1 14,855 + MMJJ_RS00075 0.14 -1.2 14,855 + MMJJ_RS00075 0.14 -0.4 14,963 + MMJJ_RS00075 0.27 -3.4 15,112 - MMJJ_RS00075 0.46 +0.6 15,112 - MMJJ_RS00075 0.46 -1.9 15,112 - MMJJ_RS00075 0.46 -1.8 15,112 - MMJJ_RS00075 0.46 -2.1 15,113 + MMJJ_RS00075 0.46 -2.1 15,113 + MMJJ_RS00075 0.46 -1.8 15,113 + MMJJ_RS00075 0.46 -2.3 15,113 + MMJJ_RS00075 0.46 -2.9 15,113 + MMJJ_RS00075 0.46 -1.9 15,118 - MMJJ_RS00075 0.47 -0.9 15,119 + MMJJ_RS00075 0.47 -1.0 15,153 - MMJJ_RS00075 0.52 -1.8 15,154 + MMJJ_RS00075 0.52 -2.6 15,154 + MMJJ_RS00075 0.52 -2.2 15,154 + +0.6 15,154 + MMJJ_RS00075 0.52 -2.4 15,164 + MMJJ_RS00075 0.53 -2.0 15,184 - MMJJ_RS00075 0.56 -1.5 15,184 - MMJJ_RS00075 0.56 -2.9 15,184 - MMJJ_RS00075 0.56 -1.6 15,256 - MMJJ_RS00075 0.65 -2.0 15,262 - MMJJ_RS00075 0.66 -2.3 15,295 - MMJJ_RS00075 0.70 -3.4 15,385 + MMJJ_RS00075 0.81 +0.6 15,409 - MMJJ_RS00075 0.84 -2.7 15,433 - MMJJ_RS00075 0.87 -1.3 15,434 + MMJJ_RS00075 0.87 -2.2 15,464 + -2.6 15,464 + -2.5 15,465 - -2.6 15,465 - -2.8 15,465 - -1.4 15,465 - -1.9 15,466 + -3.1 15,473 + -3.4 15,516 - -2.4 15,655 + MMJJ_RS00080 0.19 -1.4 15,655 + MMJJ_RS00080 0.19 -0.5 15,748 + MMJJ_RS00080 0.33 -1.8 15,748 + MMJJ_RS00080 0.33 -2.4 15,832 + MMJJ_RS00080 0.46 -1.6 15,876 - MMJJ_RS00080 0.53 +0.6 15,876 - MMJJ_RS00080 0.53 -2.4 15,876 - MMJJ_RS00080 0.53 -1.4 15,937 + MMJJ_RS00080 0.62 -2.1 15,937 + MMJJ_RS00080 0.62 -1.1 15,937 + MMJJ_RS00080 0.62 -0.8 15,937 + MMJJ_RS00080 0.62 -1.3 15,937 + MMJJ_RS00080 0.62 -1.2 15,939 - MMJJ_RS00080 0.63 -1.0 15,939 - MMJJ_RS00080 0.63 -1.8 15,939 - MMJJ_RS00080 0.63 -1.2 15,939 - MMJJ_RS00080 0.63 -0.7 15,939 - MMJJ_RS00080 0.63 -2.5 15,939 - MMJJ_RS00080 0.63 -1.7 15,939 - MMJJ_RS00080 0.63 -1.1 15,940 + MMJJ_RS00080 0.63 -1.4 15,940 + MMJJ_RS00080 0.63 -0.9 15,940 + MMJJ_RS00080 0.63 -3.0 15,941 - MMJJ_RS00080 0.63 -2.1 15,941 - MMJJ_RS00080 0.63 -1.9 15,941 - MMJJ_RS00080 0.63 -1.0 15,941 - MMJJ_RS00080 0.63 -1.7 15,942 + MMJJ_RS00080 0.63 -0.6 15,942 + MMJJ_RS00080 0.63 -3.1 15,942 + MMJJ_RS00080 0.63 -1.0 15,942 + MMJJ_RS00080 0.63 -1.4 15,944 + MMJJ_RS00080 0.63 -1.1 15,958 - MMJJ_RS00080 0.65 -1.6 15,997 + MMJJ_RS00080 0.71 -2.4 15,997 + MMJJ_RS00080 0.71 -1.4 15,999 - MMJJ_RS00080 0.72 -1.4 16,003 - MMJJ_RS00080 0.72 -1.9 16,003 - MMJJ_RS00080 0.72 -0.9 16,003 - MMJJ_RS00080 0.72 -2.1 16,004 + MMJJ_RS00080 0.72 -2.6 16,004 + MMJJ_RS00080 0.72 -3.3 16,074 + MMJJ_RS00080 0.83 -1.4 16,074 + MMJJ_RS00080 0.83 -1.3 16,100 - MMJJ_RS00080 0.87 -1.4 16,103 - MMJJ_RS00080 0.88 -1.3 16,103 - MMJJ_RS00080 0.88 -1.6 16,105 - MMJJ_RS00080 0.88 -1.5 16,127 - -1.2 16,139 + -2.1 16,139 + -1.3 16,212 - +0.6 16,212 - +1.0 16,214 - +0.1 16,215 + -0.6 16,215 + -1.1 16,226 - -0.5 16,226 - -0.4 16,226 - +0.5 16,226 - -0.7 16,226 - +0.3 16,226 - -0.3 16,226 - -0.1 16,227 + +0.0 16,227 + -0.2 16,227 + -2.5 16,227 + -1.2 16,227 + -1.4 16,227 + -0.1 16,227 + -0.6 16,227 + -2.1 16,228 - +0.7 16,228 - -0.4 16,229 + -0.5 16,229 + -0.7 16,230 - +0.2 16,230 - -0.2 16,235 + -0.7 16,266 + -0.2 16,307 - +0.3 16,307 - +1.0 16,307 - +0.9 16,307 - +1.2 16,308 + +0.5 16,308 + -0.4 16,308 + -0.1 16,336 + -0.1 16,349 + +0.3 16,400 - -0.7 16,401 + -0.1 16,401 + +0.2 16,415 - -0.6 16,468 - +0.0 16,469 + +0.8
Or see this region's nucleotide sequence