Strain Fitness in Herbaspirillum seropedicae SmR1 around HSERO_RS13270

Experiment: Cobalt chloride 0.2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHSERO_RS13265 and HSERO_RS13270 overlap by 4 nucleotidesHSERO_RS13270 and HSERO_RS13275 are separated by 143 nucleotidesHSERO_RS13275 and HSERO_RS13280 overlap by 4 nucleotides HSERO_RS13265: HSERO_RS13265 - G3E family GTPase, at 3,022,328 to 3,023,461 _RS13265 HSERO_RS13270: HSERO_RS13270 - bifunctional precorrin-3 methyltransferase/precorrin-6x reductase oxidoreductase, at 3,023,458 to 3,025,017 _RS13270 HSERO_RS13275: HSERO_RS13275 - cobalamin biosynthesis protein CbiG, at 3,025,161 to 3,025,964 _RS13275 HSERO_RS13280: HSERO_RS13280 - precorrin-4 C11-methyltransferase, at 3,025,961 to 3,026,737 _RS13280 Position (kb) 3023 3024 3025 3026Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3022.590 kb on + strand, within HSERO_RS13265at 3022.608 kb on - strand, within HSERO_RS13265at 3022.703 kb on + strandat 3022.837 kb on + strand, within HSERO_RS13265at 3022.885 kb on - strand, within HSERO_RS13265at 3022.987 kb on + strand, within HSERO_RS13265at 3023.524 kb on + strandat 3023.524 kb on + strandat 3023.693 kb on + strand, within HSERO_RS13270at 3023.817 kb on + strand, within HSERO_RS13270at 3023.950 kb on + strand, within HSERO_RS13270at 3024.276 kb on + strand, within HSERO_RS13270at 3024.284 kb on - strand, within HSERO_RS13270at 3024.308 kb on + strand, within HSERO_RS13270at 3024.452 kb on + strand, within HSERO_RS13270at 3024.540 kb on - strandat 3024.974 kb on + strandat 3025.150 kb on - strandat 3025.397 kb on - strand, within HSERO_RS13275at 3025.834 kb on - strand, within HSERO_RS13275at 3025.931 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.2 mM
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3,022,590 + HSERO_RS13265 0.23 +0.2
3,022,608 - HSERO_RS13265 0.25 +1.0
3,022,703 + +2.2
3,022,837 + HSERO_RS13265 0.45 +1.1
3,022,885 - HSERO_RS13265 0.49 -1.8
3,022,987 + HSERO_RS13265 0.58 -0.3
3,023,524 + +0.6
3,023,524 + -2.3
3,023,693 + HSERO_RS13270 0.15 +1.1
3,023,817 + HSERO_RS13270 0.23 +0.1
3,023,950 + HSERO_RS13270 0.32 +1.3
3,024,276 + HSERO_RS13270 0.52 +1.6
3,024,284 - HSERO_RS13270 0.53 +0.5
3,024,308 + HSERO_RS13270 0.54 +1.7
3,024,452 + HSERO_RS13270 0.64 -0.2
3,024,540 - +2.9
3,024,974 + +0.6
3,025,150 - +0.7
3,025,397 - HSERO_RS13275 0.29 -0.2
3,025,834 - HSERO_RS13275 0.84 -2.0
3,025,931 + +0.1

Or see this region's nucleotide sequence