Experiment: LB, 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20225 and BPHYT_RS20230 overlap by 4 nucleotides BPHYT_RS20230 and BPHYT_RS20235 overlap by 4 nucleotides BPHYT_RS20235 and BPHYT_RS20240 overlap by 4 nucleotides BPHYT_RS20240 and BPHYT_RS20245 are separated by 5 nucleotides
BPHYT_RS20225: BPHYT_RS20225 - taurine catabolism dioxygenase tauD/tfdA, at 81,933 to 82,967
_RS20225
BPHYT_RS20230: BPHYT_RS20230 - ABC transporter, at 82,964 to 83,830
_RS20230
BPHYT_RS20235: BPHYT_RS20235 - iron ABC transporter, at 83,827 to 85,935
_RS20235
BPHYT_RS20240: BPHYT_RS20240 - iron reductase, at 85,932 to 86,750
_RS20240
BPHYT_RS20245: BPHYT_RS20245 - iron ABC transporter substrate-binding protein, at 86,756 to 87,856
_RS20245
Position (kb) 83
84
85
86 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 82.914 kb on + strand at 82.915 kb on - strand at 83.809 kb on - strand at 83.884 kb on + strand at 84.160 kb on - strand, within BPHYT_RS20235 at 84.427 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.480 kb on + strand, within BPHYT_RS20235 at 85.481 kb on - strand, within BPHYT_RS20235 at 86.202 kb on + strand, within BPHYT_RS20240 at 86.203 kb on - strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.205 kb on + strand, within BPHYT_RS20240 at 86.206 kb on - strand, within BPHYT_RS20240 at 86.478 kb on + strand, within BPHYT_RS20240 at 86.650 kb on + strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.651 kb on - strand, within BPHYT_RS20240 at 86.655 kb on + strand, within BPHYT_RS20240 at 86.702 kb on - strand at 86.867 kb on + strand, within BPHYT_RS20245
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 30C remove 82,914 + -0.7 82,915 - +1.1 83,809 - -0.1 83,884 + -0.5 84,160 - BPHYT_RS20235 0.16 -0.8 84,427 + BPHYT_RS20235 0.28 -0.6 85,480 + BPHYT_RS20235 0.78 -0.9 85,480 + BPHYT_RS20235 0.78 -1.7 85,480 + BPHYT_RS20235 0.78 -1.9 85,481 - BPHYT_RS20235 0.78 -2.9 86,202 + BPHYT_RS20240 0.33 +0.1 86,203 - BPHYT_RS20240 0.33 -0.6 86,205 + BPHYT_RS20240 0.33 -0.0 86,205 + BPHYT_RS20240 0.33 +0.1 86,206 - BPHYT_RS20240 0.33 -0.9 86,478 + BPHYT_RS20240 0.67 -0.1 86,650 + BPHYT_RS20240 0.88 -0.0 86,651 - BPHYT_RS20240 0.88 -1.0 86,651 - BPHYT_RS20240 0.88 -0.7 86,651 - BPHYT_RS20240 0.88 -0.2 86,655 + BPHYT_RS20240 0.88 +0.1 86,702 - -0.3 86,867 + BPHYT_RS20245 0.10 +0.3
Or see this region's nucleotide sequence