Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01856 and SMc01855 are separated by 137 nucleotides SMc01855 and SMc01854 are separated by 198 nucleotides SMc01854 and SMc01853 overlap by 4 nucleotides
SMc01856: SMc01856 - sarcosine oxidase, at 2,357,250 to 2,358,437
SMc01856
SMc01855: SMc01855 - hypothetical protein, at 2,358,575 to 2,359,114
SMc01855
SMc01854: SMc01854 - amidase (AMPD protein), at 2,359,313 to 2,360,104
SMc01854
SMc01853: SMc01853 - hypothetical protein, at 2,360,101 to 2,360,808
SMc01853
Position (kb)
2359
2360
2361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2358.470 kb on + strand at 2358.470 kb on + strand at 2358.470 kb on + strand at 2358.471 kb on - strand at 2358.471 kb on - strand at 2358.571 kb on + strand at 2358.661 kb on - strand, within SMc01855 at 2358.717 kb on + strand, within SMc01855 at 2358.718 kb on - strand, within SMc01855 at 2358.718 kb on - strand, within SMc01855 at 2358.737 kb on - strand, within SMc01855 at 2358.775 kb on + strand, within SMc01855 at 2358.777 kb on + strand, within SMc01855 at 2358.777 kb on + strand, within SMc01855 at 2358.907 kb on - strand, within SMc01855 at 2358.907 kb on - strand, within SMc01855 at 2358.937 kb on + strand, within SMc01855 at 2358.937 kb on + strand, within SMc01855 at 2358.937 kb on + strand, within SMc01855 at 2358.938 kb on - strand, within SMc01855 at 2358.938 kb on - strand, within SMc01855 at 2358.938 kb on - strand, within SMc01855 at 2358.938 kb on - strand, within SMc01855 at 2358.938 kb on - strand, within SMc01855 at 2358.939 kb on + strand, within SMc01855 at 2358.939 kb on + strand, within SMc01855 at 2359.044 kb on + strand, within SMc01855 at 2359.044 kb on + strand, within SMc01855 at 2359.045 kb on - strand, within SMc01855 at 2359.051 kb on + strand, within SMc01855 at 2359.106 kb on + strand at 2359.106 kb on + strand at 2359.116 kb on + strand at 2359.116 kb on + strand at 2359.265 kb on + strand at 2359.265 kb on + strand at 2359.265 kb on + strand at 2359.319 kb on + strand at 2360.136 kb on - strand at 2360.368 kb on + strand, within SMc01853 at 2360.369 kb on - strand, within SMc01853 at 2360.394 kb on + strand, within SMc01853 at 2360.394 kb on + strand, within SMc01853 at 2360.394 kb on + strand, within SMc01853 at 2360.556 kb on - strand, within SMc01853 at 2360.556 kb on - strand, within SMc01853 at 2360.556 kb on - strand, within SMc01853 at 2360.809 kb on - strand at 2360.809 kb on - strand at 2360.809 kb on - strand at 2360.809 kb on - strand at 2361.038 kb on - strand at 2361.038 kb on - strand at 2361.093 kb on + strand at 2361.093 kb on + strand at 2361.094 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 2,358,470 + -0.8 2,358,470 + +0.4 2,358,470 + -0.3 2,358,471 - +0.2 2,358,471 - +0.1 2,358,571 + +0.2 2,358,661 - SMc01855 0.16 +1.9 2,358,717 + SMc01855 0.26 -2.6 2,358,718 - SMc01855 0.26 +1.3 2,358,718 - SMc01855 0.26 +0.8 2,358,737 - SMc01855 0.30 +0.2 2,358,775 + SMc01855 0.37 +1.9 2,358,777 + SMc01855 0.37 +1.0 2,358,777 + SMc01855 0.37 -0.1 2,358,907 - SMc01855 0.61 -0.0 2,358,907 - SMc01855 0.61 -0.8 2,358,937 + SMc01855 0.67 -1.0 2,358,937 + SMc01855 0.67 -1.1 2,358,937 + SMc01855 0.67 -0.4 2,358,938 - SMc01855 0.67 +1.2 2,358,938 - SMc01855 0.67 +0.2 2,358,938 - SMc01855 0.67 +0.5 2,358,938 - SMc01855 0.67 +0.6 2,358,938 - SMc01855 0.67 +1.3 2,358,939 + SMc01855 0.67 -1.9 2,358,939 + SMc01855 0.67 -0.3 2,359,044 + SMc01855 0.87 +0.3 2,359,044 + SMc01855 0.87 -0.1 2,359,045 - SMc01855 0.87 +0.3 2,359,051 + SMc01855 0.88 -0.5 2,359,106 + +0.4 2,359,106 + -0.1 2,359,116 + +0.9 2,359,116 + -1.1 2,359,265 + -0.2 2,359,265 + -0.7 2,359,265 + -0.1 2,359,319 + -0.4 2,360,136 - +0.4 2,360,368 + SMc01853 0.38 +0.5 2,360,369 - SMc01853 0.38 -0.4 2,360,394 + SMc01853 0.41 -2.1 2,360,394 + SMc01853 0.41 -1.7 2,360,394 + SMc01853 0.41 -2.0 2,360,556 - SMc01853 0.64 -1.7 2,360,556 - SMc01853 0.64 -0.1 2,360,556 - SMc01853 0.64 -1.2 2,360,809 - -0.3 2,360,809 - +0.0 2,360,809 - -0.9 2,360,809 - -0.9 2,361,038 - -0.5 2,361,038 - -0.7 2,361,093 + -3.0 2,361,093 + -1.2 2,361,094 - +0.6
Or see this region's nucleotide sequence