Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc01221 and SMc01220 overlap by 4 nucleotides SMc01220 and SMc01219 are separated by 6 nucleotides
SMc01221: SMc01221 - lipopolysaccharide core biosynthesis glycosyl transferase, at 1,702,990 to 1,704,021
SMc01221
SMc01220: SMc01220 - lipopolysaccharide core biosynthesis glycosyl transferase, at 1,704,018 to 1,705,040
SMc01220
SMc01219: SMc01219 - lipopolysaccharide core biosynthesis mannosyltransferase, at 1,705,047 to 1,706,102
SMc01219
Position (kb)
1704
1705
1706 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1703.139 kb on + strand, within SMc01221 at 1703.140 kb on - strand, within SMc01221 at 1703.146 kb on + strand, within SMc01221 at 1703.146 kb on + strand, within SMc01221 at 1703.146 kb on + strand, within SMc01221 at 1703.146 kb on + strand, within SMc01221 at 1703.146 kb on + strand, within SMc01221 at 1703.147 kb on - strand, within SMc01221 at 1703.147 kb on - strand, within SMc01221 at 1703.147 kb on - strand, within SMc01221 at 1703.237 kb on + strand, within SMc01221 at 1703.237 kb on + strand, within SMc01221 at 1703.238 kb on - strand, within SMc01221 at 1703.332 kb on + strand, within SMc01221 at 1703.335 kb on + strand, within SMc01221 at 1703.335 kb on + strand, within SMc01221 at 1703.337 kb on + strand, within SMc01221 at 1703.337 kb on + strand, within SMc01221 at 1703.337 kb on + strand, within SMc01221 at 1703.338 kb on - strand, within SMc01221 at 1703.338 kb on - strand, within SMc01221 at 1703.338 kb on - strand, within SMc01221 at 1703.529 kb on + strand, within SMc01221 at 1703.529 kb on + strand, within SMc01221 at 1703.579 kb on - strand, within SMc01221 at 1703.687 kb on - strand, within SMc01221 at 1703.687 kb on - strand, within SMc01221 at 1703.786 kb on + strand, within SMc01221 at 1703.786 kb on + strand, within SMc01221 at 1704.073 kb on + strand at 1704.074 kb on - strand at 1704.342 kb on + strand, within SMc01220 at 1704.361 kb on - strand, within SMc01220 at 1704.361 kb on - strand, within SMc01220 at 1704.442 kb on + strand, within SMc01220 at 1704.442 kb on + strand, within SMc01220 at 1704.443 kb on - strand, within SMc01220 at 1704.443 kb on - strand, within SMc01220 at 1704.569 kb on + strand, within SMc01220 at 1704.569 kb on + strand, within SMc01220 at 1704.570 kb on - strand, within SMc01220 at 1704.606 kb on + strand, within SMc01220 at 1705.011 kb on + strand at 1705.011 kb on + strand at 1705.011 kb on + strand at 1705.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 1,703,139 + SMc01221 0.14 -1.8 1,703,140 - SMc01221 0.15 -3.8 1,703,146 + SMc01221 0.15 -1.6 1,703,146 + SMc01221 0.15 -2.0 1,703,146 + SMc01221 0.15 -3.3 1,703,146 + SMc01221 0.15 -2.2 1,703,146 + SMc01221 0.15 -1.3 1,703,147 - SMc01221 0.15 -3.0 1,703,147 - SMc01221 0.15 -2.9 1,703,147 - SMc01221 0.15 -3.6 1,703,237 + SMc01221 0.24 -1.5 1,703,237 + SMc01221 0.24 -2.3 1,703,238 - SMc01221 0.24 -3.7 1,703,332 + SMc01221 0.33 -2.4 1,703,335 + SMc01221 0.33 -2.4 1,703,335 + SMc01221 0.33 -2.2 1,703,337 + SMc01221 0.34 -1.5 1,703,337 + SMc01221 0.34 +0.1 1,703,337 + SMc01221 0.34 -3.7 1,703,338 - SMc01221 0.34 -3.6 1,703,338 - SMc01221 0.34 -2.8 1,703,338 - SMc01221 0.34 -1.7 1,703,529 + SMc01221 0.52 -1.7 1,703,529 + SMc01221 0.52 -2.5 1,703,579 - SMc01221 0.57 +1.6 1,703,687 - SMc01221 0.68 -3.1 1,703,687 - SMc01221 0.68 -1.5 1,703,786 + SMc01221 0.77 -0.7 1,703,786 + SMc01221 0.77 -2.4 1,704,073 + -4.3 1,704,074 - -4.6 1,704,342 + SMc01220 0.32 -2.0 1,704,361 - SMc01220 0.34 -3.8 1,704,361 - SMc01220 0.34 -2.7 1,704,442 + SMc01220 0.41 -3.2 1,704,442 + SMc01220 0.41 +0.6 1,704,443 - SMc01220 0.42 -2.8 1,704,443 - SMc01220 0.42 -3.0 1,704,569 + SMc01220 0.54 -3.4 1,704,569 + SMc01220 0.54 -2.7 1,704,570 - SMc01220 0.54 -1.4 1,704,606 + SMc01220 0.57 -2.1 1,705,011 + -2.7 1,705,011 + -4.4 1,705,011 + -4.4 1,705,012 - -3.1
Or see this region's nucleotide sequence