Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00975 and SMc00974 are separated by 81 nucleotides SMc00974 and SMc00973 overlap by 4 nucleotides SMc00973 and SMc00972 are separated by 51 nucleotides
SMc00975: SMc00975 - chemoreceptor methyl-accepting chemotaxis transmembrane protein, at 963,594 to 965,717
SMc00975
SMc00974: SMc00974 - RNA methyltransferase, at 965,799 to 967,046
SMc00974
SMc00973: SMc00973 - hypothetical protein, at 967,043 to 967,798
SMc00973
SMc00972: SMc00972 - 1-deoxy-D-xylulose-5-phosphate synthase, at 967,850 to 969,787
SMc00972
Position (kb) 965
966
967
968 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 965.479 kb on + strand, within SMc00975 at 965.480 kb on - strand, within SMc00975 at 965.480 kb on - strand, within SMc00975 at 965.480 kb on - strand, within SMc00975 at 965.579 kb on + strand at 965.579 kb on + strand at 965.660 kb on - strand at 965.660 kb on - strand at 965.685 kb on + strand at 965.762 kb on + strand at 965.768 kb on + strand at 965.768 kb on + strand at 965.769 kb on - strand at 966.503 kb on + strand, within SMc00974 at 966.784 kb on + strand, within SMc00974 at 966.785 kb on - strand, within SMc00974 at 967.400 kb on - strand, within SMc00973 at 967.606 kb on - strand, within SMc00973 at 967.606 kb on - strand, within SMc00973 at 967.787 kb on - strand at 967.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 965,479 + SMc00975 0.89 -0.5 965,480 - SMc00975 0.89 -0.1 965,480 - SMc00975 0.89 -0.1 965,480 - SMc00975 0.89 -1.6 965,579 + +0.1 965,579 + -0.8 965,660 - +0.0 965,660 - +0.8 965,685 + -2.9 965,762 + -0.3 965,768 + +0.8 965,768 + -0.2 965,769 - -0.6 966,503 + SMc00974 0.56 -0.6 966,784 + SMc00974 0.79 +0.0 966,785 - SMc00974 0.79 -0.1 967,400 - SMc00973 0.47 +2.2 967,606 - SMc00973 0.74 +1.8 967,606 - SMc00973 0.74 +0.5 967,787 - +0.3 967,787 - +0.2
Or see this region's nucleotide sequence