Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21513 and SM_b21514 are separated by 123 nucleotides SM_b21514 and SM_b21515 overlap by 4 nucleotides SM_b21515 and SM_b21516 are separated by 253 nucleotides
SM_b21513: SM_b21513 - cell-surface polysaccharide exporter protein PST family, at 1,436,757 to 1,438,067
_b21513
SM_b21514: SM_b21514 - modification methylase, at 1,438,191 to 1,439,018
_b21514
SM_b21515: SM_b21515 - hypothetical protein, at 1,439,015 to 1,440,136
_b21515
SM_b21516: SM_b21516 - hypothetical protein, at 1,440,390 to 1,441,121
_b21516
Position (kb)
1439
1440
1441 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1438.091 kb on + strand at 1438.092 kb on - strand at 1438.092 kb on - strand at 1438.143 kb on + strand at 1438.152 kb on + strand at 1438.168 kb on + strand at 1438.173 kb on + strand at 1438.173 kb on + strand at 1438.174 kb on - strand at 1438.174 kb on - strand at 1438.238 kb on + strand at 1438.695 kb on + strand, within SM_b21514 at 1438.696 kb on - strand, within SM_b21514 at 1438.698 kb on - strand, within SM_b21514 at 1438.777 kb on - strand, within SM_b21514 at 1438.783 kb on + strand, within SM_b21514 at 1438.783 kb on + strand, within SM_b21514 at 1438.784 kb on - strand, within SM_b21514 at 1438.784 kb on - strand, within SM_b21514 at 1438.909 kb on - strand, within SM_b21514 at 1438.909 kb on - strand, within SM_b21514 at 1438.909 kb on - strand, within SM_b21514 at 1438.909 kb on - strand, within SM_b21514 at 1439.047 kb on + strand at 1439.138 kb on + strand, within SM_b21515 at 1439.139 kb on - strand, within SM_b21515 at 1439.139 kb on - strand, within SM_b21515 at 1439.141 kb on - strand, within SM_b21515 at 1439.141 kb on - strand at 1439.183 kb on + strand, within SM_b21515 at 1439.243 kb on + strand, within SM_b21515 at 1439.435 kb on + strand, within SM_b21515 at 1439.435 kb on + strand, within SM_b21515 at 1439.436 kb on - strand, within SM_b21515 at 1439.728 kb on + strand, within SM_b21515 at 1439.728 kb on + strand, within SM_b21515 at 1439.728 kb on + strand, within SM_b21515 at 1439.907 kb on + strand, within SM_b21515 at 1439.907 kb on + strand, within SM_b21515 at 1439.908 kb on - strand, within SM_b21515 at 1439.908 kb on - strand, within SM_b21515 at 1439.989 kb on + strand, within SM_b21515 at 1440.135 kb on - strand at 1440.135 kb on - strand at 1440.260 kb on + strand at 1440.264 kb on + strand at 1440.264 kb on + strand at 1440.264 kb on + strand at 1440.264 kb on + strand at 1440.265 kb on - strand at 1440.265 kb on - strand at 1440.265 kb on - strand at 1440.300 kb on + strand at 1440.391 kb on + strand at 1440.392 kb on - strand at 1440.392 kb on - strand at 1440.392 kb on - strand at 1440.445 kb on + strand at 1440.451 kb on - strand at 1440.451 kb on - strand at 1440.553 kb on + strand, within SM_b21516 at 1440.583 kb on + strand, within SM_b21516 at 1440.583 kb on + strand, within SM_b21516 at 1440.583 kb on + strand, within SM_b21516 at 1440.583 kb on + strand, within SM_b21516 at 1440.583 kb on + strand, within SM_b21516 at 1440.584 kb on - strand, within SM_b21516 at 1440.677 kb on + strand, within SM_b21516 at 1440.677 kb on + strand, within SM_b21516 at 1440.720 kb on + strand, within SM_b21516 at 1440.720 kb on + strand, within SM_b21516 at 1440.720 kb on + strand, within SM_b21516 at 1440.721 kb on - strand, within SM_b21516 at 1440.721 kb on - strand, within SM_b21516 at 1440.806 kb on - strand, within SM_b21516 at 1440.902 kb on + strand, within SM_b21516 at 1440.903 kb on - strand, within SM_b21516 at 1440.904 kb on + strand, within SM_b21516 at 1440.904 kb on + strand, within SM_b21516 at 1440.949 kb on + strand, within SM_b21516 at 1440.950 kb on - strand, within SM_b21516 at 1440.950 kb on - strand, within SM_b21516 at 1441.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 1,438,091 + -0.3 1,438,092 - +0.2 1,438,092 - +1.0 1,438,143 + +0.2 1,438,152 + +0.3 1,438,168 + +0.1 1,438,173 + -0.1 1,438,173 + +0.1 1,438,174 - -0.3 1,438,174 - +0.4 1,438,238 + -0.6 1,438,695 + SM_b21514 0.61 +1.0 1,438,696 - SM_b21514 0.61 +0.4 1,438,698 - SM_b21514 0.61 +0.1 1,438,777 - SM_b21514 0.71 -0.0 1,438,783 + SM_b21514 0.71 -2.1 1,438,783 + SM_b21514 0.71 +0.5 1,438,784 - SM_b21514 0.72 -0.1 1,438,784 - SM_b21514 0.72 +0.6 1,438,909 - SM_b21514 0.87 +0.5 1,438,909 - SM_b21514 0.87 -1.7 1,438,909 - SM_b21514 0.87 +2.3 1,438,909 - SM_b21514 0.87 +0.5 1,439,047 + -0.6 1,439,138 + SM_b21515 0.11 +0.0 1,439,139 - SM_b21515 0.11 -0.6 1,439,139 - SM_b21515 0.11 -0.5 1,439,141 - SM_b21515 0.11 +0.6 1,439,141 - +0.8 1,439,183 + SM_b21515 0.15 -1.2 1,439,243 + SM_b21515 0.20 +0.1 1,439,435 + SM_b21515 0.37 +0.2 1,439,435 + SM_b21515 0.37 -0.5 1,439,436 - SM_b21515 0.38 +3.4 1,439,728 + SM_b21515 0.64 -0.5 1,439,728 + SM_b21515 0.64 +0.4 1,439,728 + SM_b21515 0.64 +0.5 1,439,907 + SM_b21515 0.80 -0.7 1,439,907 + SM_b21515 0.80 +1.1 1,439,908 - SM_b21515 0.80 +0.3 1,439,908 - SM_b21515 0.80 +0.8 1,439,989 + SM_b21515 0.87 +0.6 1,440,135 - +1.2 1,440,135 - -0.5 1,440,260 + +0.4 1,440,264 + +0.5 1,440,264 + +0.1 1,440,264 + +0.2 1,440,264 + -0.5 1,440,265 - +0.9 1,440,265 - +1.1 1,440,265 - -1.6 1,440,300 + +1.7 1,440,391 + +1.2 1,440,392 - -1.0 1,440,392 - +0.6 1,440,392 - -0.9 1,440,445 + +0.8 1,440,451 - -0.9 1,440,451 - -0.7 1,440,553 + SM_b21516 0.22 -1.6 1,440,583 + SM_b21516 0.26 -0.6 1,440,583 + SM_b21516 0.26 +0.2 1,440,583 + SM_b21516 0.26 -0.8 1,440,583 + SM_b21516 0.26 -0.8 1,440,583 + SM_b21516 0.26 -1.0 1,440,584 - SM_b21516 0.27 -0.2 1,440,677 + SM_b21516 0.39 -0.5 1,440,677 + SM_b21516 0.39 -1.3 1,440,720 + SM_b21516 0.45 -0.7 1,440,720 + SM_b21516 0.45 -0.3 1,440,720 + SM_b21516 0.45 -1.6 1,440,721 - SM_b21516 0.45 -1.2 1,440,721 - SM_b21516 0.45 -0.6 1,440,806 - SM_b21516 0.57 -0.3 1,440,902 + SM_b21516 0.70 +0.9 1,440,903 - SM_b21516 0.70 -0.8 1,440,904 + SM_b21516 0.70 +0.0 1,440,904 + SM_b21516 0.70 -0.5 1,440,949 + SM_b21516 0.76 -1.0 1,440,950 - SM_b21516 0.77 -0.4 1,440,950 - SM_b21516 0.77 -0.1 1,441,102 - -1.6
Or see this region's nucleotide sequence