Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21311 and SM_b21312 overlap by 4 nucleotides SM_b21312 and SM_b21313 overlap by 4 nucleotides SM_b21313 and SM_b21314 are separated by 130 nucleotides
SM_b21311: SM_b21311 - glycosyltransferase, at 975,102 to 976,364
_b21311
SM_b21312: SM_b21312 - methyltransferase, at 976,361 to 977,206
_b21312
SM_b21313: SM_b21313 - bifunctional glycosyltransferase, at 977,203 to 979,452
_b21313
SM_b21314: SM_b21314 - secreted calcium-binding protein, at 979,583 to 980,242
_b21314
Position (kb) 977
978
979
980 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 976.273 kb on + strand at 976.274 kb on - strand at 976.334 kb on + strand at 976.335 kb on - strand at 976.404 kb on + strand at 976.404 kb on + strand at 976.405 kb on - strand at 976.536 kb on + strand, within SM_b21312 at 976.536 kb on + strand, within SM_b21312 at 976.536 kb on + strand, within SM_b21312 at 976.683 kb on + strand, within SM_b21312 at 976.684 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.804 kb on - strand, within SM_b21312 at 976.911 kb on + strand, within SM_b21312 at 976.912 kb on - strand, within SM_b21312 at 977.089 kb on + strand, within SM_b21312 at 977.331 kb on - strand at 977.354 kb on + strand at 977.354 kb on + strand at 977.355 kb on - strand at 977.355 kb on - strand at 977.355 kb on - strand at 977.393 kb on + strand at 977.414 kb on + strand at 977.421 kb on + strand at 977.483 kb on + strand, within SM_b21313 at 977.484 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.514 kb on - strand, within SM_b21313 at 977.585 kb on + strand, within SM_b21313 at 977.626 kb on + strand, within SM_b21313 at 977.627 kb on - strand, within SM_b21313 at 977.627 kb on - strand, within SM_b21313 at 977.954 kb on + strand, within SM_b21313 at 977.955 kb on - strand, within SM_b21313 at 977.955 kb on - strand, within SM_b21313 at 978.035 kb on + strand, within SM_b21313 at 978.036 kb on - strand, within SM_b21313 at 978.036 kb on - strand, within SM_b21313 at 978.095 kb on + strand, within SM_b21313 at 978.095 kb on + strand, within SM_b21313 at 978.096 kb on - strand, within SM_b21313 at 978.206 kb on - strand, within SM_b21313 at 978.404 kb on + strand at 978.450 kb on - strand, within SM_b21313 at 978.450 kb on - strand, within SM_b21313 at 978.548 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.893 kb on + strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.894 kb on - strand, within SM_b21313 at 978.945 kb on - strand, within SM_b21313 at 979.097 kb on - strand, within SM_b21313 at 979.370 kb on - strand at 979.581 kb on + strand at 979.582 kb on - strand at 979.600 kb on - strand at 979.703 kb on - strand, within SM_b21314 at 979.747 kb on + strand, within SM_b21314 at 979.747 kb on + strand, within SM_b21314 at 979.757 kb on + strand, within SM_b21314 at 979.758 kb on - strand, within SM_b21314 at 979.832 kb on - strand, within SM_b21314 at 979.832 kb on - strand, within SM_b21314 at 979.987 kb on - strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.020 kb on + strand, within SM_b21314 at 980.040 kb on + strand, within SM_b21314 at 980.040 kb on + strand, within SM_b21314 at 980.041 kb on - strand, within SM_b21314 at 980.041 kb on - strand, within SM_b21314
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 976,273 + -1.0 976,274 - -0.9 976,334 + +1.7 976,335 - +0.3 976,404 + -0.2 976,404 + -0.2 976,405 - +0.4 976,536 + SM_b21312 0.21 +0.0 976,536 + SM_b21312 0.21 -1.1 976,536 + SM_b21312 0.21 -0.2 976,683 + SM_b21312 0.38 -0.2 976,684 - SM_b21312 0.38 +0.5 976,804 - SM_b21312 0.52 -0.1 976,804 - SM_b21312 0.52 +0.5 976,804 - SM_b21312 0.52 -0.6 976,911 + SM_b21312 0.65 +0.3 976,912 - SM_b21312 0.65 -0.8 977,089 + SM_b21312 0.86 +0.5 977,331 - -0.5 977,354 + +0.5 977,354 + +0.3 977,355 - +0.9 977,355 - -0.1 977,355 - +0.0 977,393 + +0.3 977,414 + -0.1 977,421 + +0.2 977,483 + SM_b21313 0.12 -0.4 977,484 - SM_b21313 0.12 +0.5 977,514 - SM_b21313 0.14 -0.4 977,514 - SM_b21313 0.14 -0.7 977,514 - SM_b21313 0.14 -0.2 977,585 + SM_b21313 0.17 -1.5 977,626 + SM_b21313 0.19 -0.1 977,627 - SM_b21313 0.19 +1.7 977,627 - SM_b21313 0.19 +0.0 977,954 + SM_b21313 0.33 -0.8 977,955 - SM_b21313 0.33 -0.2 977,955 - SM_b21313 0.33 -0.3 978,035 + SM_b21313 0.37 -0.9 978,036 - SM_b21313 0.37 -0.1 978,036 - SM_b21313 0.37 -0.5 978,095 + SM_b21313 0.40 +0.3 978,095 + SM_b21313 0.40 -0.8 978,096 - SM_b21313 0.40 +0.1 978,206 - SM_b21313 0.45 +0.5 978,404 + +0.4 978,450 - SM_b21313 0.55 -0.2 978,450 - SM_b21313 0.55 -0.7 978,548 + SM_b21313 0.60 +0.3 978,893 + SM_b21313 0.75 -0.4 978,893 + SM_b21313 0.75 -0.2 978,893 + SM_b21313 0.75 -1.3 978,893 + SM_b21313 0.75 +2.8 978,894 - SM_b21313 0.75 -0.7 978,894 - SM_b21313 0.75 -0.4 978,894 - SM_b21313 0.75 +0.1 978,894 - SM_b21313 0.75 -0.9 978,945 - SM_b21313 0.77 -0.0 979,097 - SM_b21313 0.84 -0.3 979,370 - +0.1 979,581 + -0.5 979,582 - -1.0 979,600 - -0.6 979,703 - SM_b21314 0.18 -0.5 979,747 + SM_b21314 0.25 -0.0 979,747 + SM_b21314 0.25 -0.5 979,757 + SM_b21314 0.26 -0.2 979,758 - SM_b21314 0.27 +0.3 979,832 - SM_b21314 0.38 -0.7 979,832 - SM_b21314 0.38 -0.2 979,987 - SM_b21314 0.61 -0.3 980,020 + SM_b21314 0.66 -0.5 980,020 + SM_b21314 0.66 -2.1 980,020 + SM_b21314 0.66 -0.2 980,040 + SM_b21314 0.69 +0.1 980,040 + SM_b21314 0.69 -0.3 980,041 - SM_b21314 0.69 -1.1 980,041 - SM_b21314 0.69 -0.5
Or see this region's nucleotide sequence