Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20856 and SM_b20857 are separated by 243 nucleotides SM_b20857 and SM_b20858 are separated by 156 nucleotides
SM_b20856: SM_b20856 - sugar uptake ABC transporter substrate-binding protein precursor, at 1,260,905 to 1,261,873
_b20856
SM_b20857: SM_b20857 - glucose-6-phosphate isomerase, at 1,262,117 to 1,262,719
_b20857
SM_b20858: SM_b20858 - hypothetical protein, at 1,262,876 to 1,263,361
_b20858
Position (kb)
1262
1263 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1261.147 kb on - strand, within SM_b20856 at 1261.406 kb on + strand, within SM_b20856 at 1261.696 kb on + strand, within SM_b20856 at 1261.697 kb on - strand, within SM_b20856 at 1261.697 kb on - strand, within SM_b20856 at 1261.697 kb on - strand, within SM_b20856 at 1261.756 kb on - strand, within SM_b20856 at 1261.773 kb on + strand, within SM_b20856 at 1261.774 kb on - strand, within SM_b20856 at 1261.774 kb on - strand, within SM_b20856 at 1262.124 kb on + strand at 1262.125 kb on - strand at 1262.125 kb on - strand at 1262.192 kb on + strand, within SM_b20857 at 1262.193 kb on - strand, within SM_b20857 at 1262.193 kb on - strand, within SM_b20857 at 1262.226 kb on - strand, within SM_b20857 at 1262.244 kb on - strand, within SM_b20857 at 1262.244 kb on - strand, within SM_b20857 at 1262.254 kb on + strand, within SM_b20857 at 1262.283 kb on - strand, within SM_b20857 at 1262.283 kb on - strand, within SM_b20857 at 1262.372 kb on + strand, within SM_b20857 at 1262.424 kb on - strand, within SM_b20857 at 1262.506 kb on + strand, within SM_b20857 at 1262.506 kb on + strand, within SM_b20857 at 1262.507 kb on - strand, within SM_b20857 at 1262.507 kb on - strand, within SM_b20857 at 1262.507 kb on - strand, within SM_b20857 at 1262.507 kb on - strand, within SM_b20857 at 1262.513 kb on + strand, within SM_b20857 at 1262.513 kb on + strand, within SM_b20857 at 1262.513 kb on + strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.514 kb on - strand, within SM_b20857 at 1262.568 kb on + strand, within SM_b20857 at 1262.568 kb on + strand, within SM_b20857 at 1262.568 kb on + strand, within SM_b20857 at 1262.569 kb on - strand, within SM_b20857 at 1262.606 kb on + strand, within SM_b20857 at 1262.643 kb on + strand, within SM_b20857 at 1262.644 kb on - strand, within SM_b20857 at 1262.718 kb on - strand at 1262.718 kb on - strand at 1262.869 kb on + strand at 1262.869 kb on + strand at 1263.135 kb on + strand, within SM_b20858 at 1263.135 kb on + strand, within SM_b20858 at 1263.206 kb on + strand, within SM_b20858 at 1263.206 kb on + strand, within SM_b20858 at 1263.206 kb on + strand, within SM_b20858 at 1263.206 kb on + strand, within SM_b20858 at 1263.207 kb on - strand, within SM_b20858 at 1263.207 kb on - strand, within SM_b20858 at 1263.207 kb on - strand, within SM_b20858 at 1263.207 kb on - strand, within SM_b20858 at 1263.207 kb on - strand, within SM_b20858 at 1263.368 kb on + strand at 1263.403 kb on + strand at 1263.472 kb on - strand at 1263.481 kb on - strand at 1263.540 kb on + strand at 1263.544 kb on - strand at 1263.544 kb on - strand at 1263.583 kb on + strand at 1263.662 kb on + strand at 1263.662 kb on + strand at 1263.674 kb on + strand at 1263.674 kb on + strand at 1263.675 kb on - strand at 1263.675 kb on - strand at 1263.675 kb on - strand at 1263.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 1,261,147 - SM_b20856 0.25 +1.1 1,261,406 + SM_b20856 0.52 -0.4 1,261,696 + SM_b20856 0.82 +0.4 1,261,697 - SM_b20856 0.82 -0.1 1,261,697 - SM_b20856 0.82 -0.9 1,261,697 - SM_b20856 0.82 +0.7 1,261,756 - SM_b20856 0.88 +0.2 1,261,773 + SM_b20856 0.90 -0.8 1,261,774 - SM_b20856 0.90 -0.5 1,261,774 - SM_b20856 0.90 +0.3 1,262,124 + -0.8 1,262,125 - -0.1 1,262,125 - -0.3 1,262,192 + SM_b20857 0.12 +0.4 1,262,193 - SM_b20857 0.13 -0.1 1,262,193 - SM_b20857 0.13 +0.3 1,262,226 - SM_b20857 0.18 -0.1 1,262,244 - SM_b20857 0.21 +0.2 1,262,244 - SM_b20857 0.21 +0.3 1,262,254 + SM_b20857 0.23 +0.7 1,262,283 - SM_b20857 0.28 +0.2 1,262,283 - SM_b20857 0.28 -0.7 1,262,372 + SM_b20857 0.42 -0.0 1,262,424 - SM_b20857 0.51 -0.3 1,262,506 + SM_b20857 0.65 -0.9 1,262,506 + SM_b20857 0.65 -0.2 1,262,507 - SM_b20857 0.65 +0.1 1,262,507 - SM_b20857 0.65 +2.6 1,262,507 - SM_b20857 0.65 -0.2 1,262,507 - SM_b20857 0.65 +2.0 1,262,513 + SM_b20857 0.66 +0.4 1,262,513 + SM_b20857 0.66 +0.3 1,262,513 + SM_b20857 0.66 +0.0 1,262,514 - SM_b20857 0.66 +0.7 1,262,514 - SM_b20857 0.66 -0.1 1,262,514 - SM_b20857 0.66 +0.1 1,262,514 - SM_b20857 0.66 +0.1 1,262,514 - SM_b20857 0.66 +2.0 1,262,514 - SM_b20857 0.66 +0.5 1,262,568 + SM_b20857 0.75 +0.2 1,262,568 + SM_b20857 0.75 +0.7 1,262,568 + SM_b20857 0.75 -0.4 1,262,569 - SM_b20857 0.75 -0.5 1,262,606 + SM_b20857 0.81 +0.1 1,262,643 + SM_b20857 0.87 -0.4 1,262,644 - SM_b20857 0.87 +0.1 1,262,718 - +0.2 1,262,718 - +1.0 1,262,869 + +0.2 1,262,869 + -0.7 1,263,135 + SM_b20858 0.53 -0.2 1,263,135 + SM_b20858 0.53 -0.0 1,263,206 + SM_b20858 0.68 +0.8 1,263,206 + SM_b20858 0.68 -0.3 1,263,206 + SM_b20858 0.68 -0.0 1,263,206 + SM_b20858 0.68 -0.1 1,263,207 - SM_b20858 0.68 -0.2 1,263,207 - SM_b20858 0.68 -0.5 1,263,207 - SM_b20858 0.68 -0.8 1,263,207 - SM_b20858 0.68 -1.8 1,263,207 - SM_b20858 0.68 +1.2 1,263,368 + +0.4 1,263,403 + +0.4 1,263,472 - -0.0 1,263,481 - -0.4 1,263,540 + -0.6 1,263,544 - -0.2 1,263,544 - -0.5 1,263,583 + -1.2 1,263,662 + +0.5 1,263,662 + -0.4 1,263,674 + -0.1 1,263,674 + -0.8 1,263,675 - -0.6 1,263,675 - +0.4 1,263,675 - +3.1 1,263,702 - +0.2
Or see this region's nucleotide sequence