Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20134 and SM_b20135 are separated by 78 nucleotides SM_b20135 and SM_b20136 are separated by 30 nucleotides SM_b20136 and SM_b20137 are separated by 141 nucleotides
SM_b20134: SM_b20134 - permease, at 147,547 to 148,938
_b20134
SM_b20135: SM_b20135 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 149,017 to 149,649
_b20135
SM_b20136: SM_b20136 - hypothetical protein, at 149,680 to 151,053
_b20136
SM_b20137: SM_b20137 - hypothetical protein, at 151,195 to 152,136
_b20137
Position (kb) 149
150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2 at 148.847 kb on + strand at 148.848 kb on - strand at 148.848 kb on - strand at 148.912 kb on - strand at 148.933 kb on - strand at 149.174 kb on + strand, within SM_b20135 at 149.174 kb on + strand, within SM_b20135 at 149.174 kb on + strand, within SM_b20135 at 149.302 kb on + strand, within SM_b20135 at 149.336 kb on - strand, within SM_b20135 at 149.582 kb on + strand, within SM_b20135 at 149.583 kb on - strand, within SM_b20135 at 149.583 kb on - strand, within SM_b20135 at 149.583 kb on - strand, within SM_b20135 at 149.583 kb on - strand, within SM_b20135 at 149.584 kb on + strand, within SM_b20135 at 149.672 kb on + strand at 149.681 kb on + strand at 149.681 kb on + strand at 149.682 kb on - strand at 149.682 kb on - strand at 149.961 kb on - strand, within SM_b20136 at 150.032 kb on + strand, within SM_b20136 at 150.032 kb on + strand, within SM_b20136 at 150.032 kb on + strand, within SM_b20136 at 150.033 kb on - strand, within SM_b20136 at 150.129 kb on + strand, within SM_b20136 at 150.249 kb on - strand, within SM_b20136 at 150.327 kb on - strand, within SM_b20136 at 150.361 kb on + strand, within SM_b20136 at 150.361 kb on + strand, within SM_b20136 at 150.362 kb on - strand, within SM_b20136 at 150.590 kb on + strand, within SM_b20136 at 150.634 kb on + strand, within SM_b20136 at 150.634 kb on + strand, within SM_b20136 at 150.635 kb on - strand, within SM_b20136 at 150.650 kb on + strand at 150.651 kb on - strand, within SM_b20136 at 150.785 kb on + strand, within SM_b20136 at 150.785 kb on + strand, within SM_b20136 at 150.985 kb on + strand at 150.986 kb on - strand at 150.998 kb on + strand at 150.999 kb on - strand at 151.054 kb on - strand at 151.056 kb on + strand at 151.056 kb on + strand at 151.057 kb on - strand at 151.057 kb on - strand at 151.206 kb on + strand at 151.207 kb on - strand at 151.238 kb on + strand at 151.238 kb on + strand at 151.239 kb on - strand at 151.242 kb on + strand at 151.518 kb on - strand, within SM_b20137 at 151.562 kb on + strand, within SM_b20137 at 151.655 kb on + strand, within SM_b20137 at 151.655 kb on + strand, within SM_b20137 at 151.655 kb on + strand, within SM_b20137 at 151.655 kb on + strand, within SM_b20137 at 151.655 kb on + strand at 151.656 kb on - strand, within SM_b20137 at 151.656 kb on - strand, within SM_b20137 at 151.853 kb on + strand, within SM_b20137 at 151.853 kb on + strand, within SM_b20137 at 151.907 kb on + strand, within SM_b20137 at 151.908 kb on - strand, within SM_b20137 at 151.908 kb on - strand, within SM_b20137 at 151.916 kb on + strand, within SM_b20137 at 151.965 kb on + strand, within SM_b20137 at 152.025 kb on + strand, within SM_b20137 at 152.026 kb on - strand, within SM_b20137 at 152.027 kb on + strand, within SM_b20137 at 152.027 kb on + strand, within SM_b20137 at 152.027 kb on + strand, within SM_b20137 at 152.027 kb on + strand, within SM_b20137 at 152.027 kb on + strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137 at 152.028 kb on - strand, within SM_b20137
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 148,847 + -0.0 148,848 - -0.4 148,848 - -1.7 148,912 - +0.3 148,933 - -0.5 149,174 + SM_b20135 0.25 +0.9 149,174 + SM_b20135 0.25 -0.5 149,174 + SM_b20135 0.25 -0.7 149,302 + SM_b20135 0.45 -0.9 149,336 - SM_b20135 0.50 -0.1 149,582 + SM_b20135 0.89 +0.2 149,583 - SM_b20135 0.89 -0.4 149,583 - SM_b20135 0.89 -0.8 149,583 - SM_b20135 0.89 -0.8 149,583 - SM_b20135 0.89 +0.2 149,584 + SM_b20135 0.90 -2.3 149,672 + +1.7 149,681 + -0.6 149,681 + -1.8 149,682 - -0.1 149,682 - +0.5 149,961 - SM_b20136 0.20 -0.2 150,032 + SM_b20136 0.26 -0.8 150,032 + SM_b20136 0.26 +0.2 150,032 + SM_b20136 0.26 -0.9 150,033 - SM_b20136 0.26 +0.0 150,129 + SM_b20136 0.33 -1.0 150,249 - SM_b20136 0.41 +0.0 150,327 - SM_b20136 0.47 -0.4 150,361 + SM_b20136 0.50 +0.3 150,361 + SM_b20136 0.50 -0.1 150,362 - SM_b20136 0.50 +1.0 150,590 + SM_b20136 0.66 -0.3 150,634 + SM_b20136 0.69 +0.4 150,634 + SM_b20136 0.69 +1.9 150,635 - SM_b20136 0.70 +2.6 150,650 + +0.9 150,651 - SM_b20136 0.71 +1.5 150,785 + SM_b20136 0.80 +0.5 150,785 + SM_b20136 0.80 +0.4 150,985 + -0.1 150,986 - +0.4 150,998 + -0.6 150,999 - -0.6 151,054 - +0.4 151,056 + +0.4 151,056 + -1.2 151,057 - -0.0 151,057 - -0.6 151,206 + +0.1 151,207 - +0.5 151,238 + -0.7 151,238 + +0.0 151,239 - -0.1 151,242 + -0.3 151,518 - SM_b20137 0.34 -0.4 151,562 + SM_b20137 0.39 -1.6 151,655 + SM_b20137 0.49 -0.6 151,655 + SM_b20137 0.49 -1.6 151,655 + SM_b20137 0.49 +0.4 151,655 + SM_b20137 0.49 -0.4 151,655 + +0.9 151,656 - SM_b20137 0.49 -0.1 151,656 - SM_b20137 0.49 -1.1 151,853 + SM_b20137 0.70 +0.0 151,853 + SM_b20137 0.70 -0.2 151,907 + SM_b20137 0.76 +0.9 151,908 - SM_b20137 0.76 -0.0 151,908 - SM_b20137 0.76 -0.2 151,916 + SM_b20137 0.77 +0.3 151,965 + SM_b20137 0.82 -0.0 152,025 + SM_b20137 0.88 -0.3 152,026 - SM_b20137 0.88 +0.5 152,027 + SM_b20137 0.88 -0.1 152,027 + SM_b20137 0.88 +0.0 152,027 + SM_b20137 0.88 -0.1 152,027 + SM_b20137 0.88 +0.1 152,027 + SM_b20137 0.88 -0.7 152,028 - SM_b20137 0.88 -0.5 152,028 - SM_b20137 0.88 +0.1 152,028 - SM_b20137 0.88 +0.1 152,028 - SM_b20137 0.88 +1.9 152,028 - SM_b20137 0.88 +0.0 152,028 - SM_b20137 0.88 -0.6
Or see this region's nucleotide sequence