Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25525

Experiment: pyruvate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25515 and RR42_RS25520 are separated by 50 nucleotidesRR42_RS25520 and RR42_RS25525 overlap by 4 nucleotidesRR42_RS25525 and RR42_RS25530 are separated by 158 nucleotidesRR42_RS25530 and RR42_RS25535 are separated by 132 nucleotides RR42_RS25515: RR42_RS25515 - AMP-dependent synthetase, at 1,069,867 to 1,071,327 _RS25515 RR42_RS25520: RR42_RS25520 - alpha/beta hydrolase, at 1,071,378 to 1,072,310 _RS25520 RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281 _RS25525 RR42_RS25530: RR42_RS25530 - TetR family transcriptional regulator, at 1,073,440 to 1,074,090 _RS25530 RR42_RS25535: RR42_RS25535 - TetR family transcriptional regulator, at 1,074,223 to 1,074,834 _RS25535 Position (kb) 1072 1073 1074Strain fitness (log2 ratio) -2 -1 0 1 2at 1071.340 kb on + strandat 1071.340 kb on + strandat 1071.341 kb on - strandat 1071.344 kb on + strandat 1071.454 kb on + strandat 1071.455 kb on - strandat 1071.685 kb on + strand, within RR42_RS25520at 1071.807 kb on - strand, within RR42_RS25520at 1071.840 kb on - strand, within RR42_RS25520at 1071.942 kb on - strand, within RR42_RS25520at 1072.017 kb on + strand, within RR42_RS25520at 1072.018 kb on - strand, within RR42_RS25520at 1072.079 kb on - strand, within RR42_RS25520at 1072.079 kb on - strand, within RR42_RS25520at 1072.229 kb on - strandat 1072.365 kb on + strandat 1072.522 kb on - strand, within RR42_RS25525at 1072.573 kb on - strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.760 kb on + strand, within RR42_RS25525at 1072.761 kb on - strand, within RR42_RS25525at 1072.807 kb on + strand, within RR42_RS25525at 1073.028 kb on - strand, within RR42_RS25525at 1073.111 kb on + strand, within RR42_RS25525at 1073.112 kb on - strand, within RR42_RS25525at 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.243 kb on + strandat 1073.244 kb on - strandat 1073.409 kb on + strandat 1073.439 kb on + strandat 1073.440 kb on - strandat 1073.440 kb on - strandat 1073.451 kb on + strandat 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.578 kb on + strand, within RR42_RS25530at 1073.579 kb on - strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.693 kb on + strand, within RR42_RS25530at 1073.746 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.782 kb on + strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.783 kb on - strand, within RR42_RS25530at 1073.784 kb on + strand, within RR42_RS25530at 1073.854 kb on + strand, within RR42_RS25530at 1073.855 kb on - strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530at 1073.933 kb on + strand, within RR42_RS25530

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Per-strain Table

Position Strand Gene LocusTag Fraction pyruvate (C)
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1,071,340 + +0.3
1,071,340 + +1.7
1,071,341 - +0.6
1,071,344 + -0.7
1,071,454 + -1.2
1,071,455 - +0.6
1,071,685 + RR42_RS25520 0.33 -0.7
1,071,807 - RR42_RS25520 0.46 +0.2
1,071,840 - RR42_RS25520 0.50 -0.5
1,071,942 - RR42_RS25520 0.60 -0.7
1,072,017 + RR42_RS25520 0.68 -2.0
1,072,018 - RR42_RS25520 0.69 -1.2
1,072,079 - RR42_RS25520 0.75 -0.4
1,072,079 - RR42_RS25520 0.75 +0.1
1,072,229 - +0.6
1,072,365 + +1.1
1,072,522 - RR42_RS25525 0.22 -1.5
1,072,573 - RR42_RS25525 0.27 -0.4
1,072,760 + RR42_RS25525 0.46 -0.9
1,072,760 + RR42_RS25525 0.46 +0.2
1,072,761 - RR42_RS25525 0.47 -0.9
1,072,807 + RR42_RS25525 0.51 -0.9
1,073,028 - RR42_RS25525 0.74 +0.5
1,073,111 + RR42_RS25525 0.82 -1.6
1,073,112 - RR42_RS25525 0.83 -0.8
1,073,243 + -0.2
1,073,243 + +0.9
1,073,243 + -0.8
1,073,244 - -0.3
1,073,409 + -0.1
1,073,439 + +0.6
1,073,440 - -0.1
1,073,440 - +1.0
1,073,451 + +0.6
1,073,578 + RR42_RS25530 0.21 -0.1
1,073,578 + RR42_RS25530 0.21 -1.2
1,073,578 + RR42_RS25530 0.21 +0.9
1,073,579 - RR42_RS25530 0.21 -0.2
1,073,693 + RR42_RS25530 0.39 +0.6
1,073,693 + RR42_RS25530 0.39 +1.1
1,073,746 + RR42_RS25530 0.47 +0.8
1,073,782 + RR42_RS25530 0.53 +2.1
1,073,782 + RR42_RS25530 0.53 +0.1
1,073,783 - RR42_RS25530 0.53 -0.4
1,073,783 - RR42_RS25530 0.53 -0.1
1,073,783 - RR42_RS25530 0.53 -0.8
1,073,783 - RR42_RS25530 0.53 -0.6
1,073,784 + RR42_RS25530 0.53 +1.1
1,073,854 + RR42_RS25530 0.64 -0.1
1,073,855 - RR42_RS25530 0.64 -1.2
1,073,933 + RR42_RS25530 0.76 -0.2
1,073,933 + RR42_RS25530 0.76 -0.3

Or see this region's nucleotide sequence