Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02728 and SMc02729 are separated by 90 nucleotides SMc02729 and SMc02730 are separated by 3 nucleotides
SMc02728: SMc02728 - formate--tetrahydrofolate ligase, at 2,579,562 to 2,581,241
SMc02728
SMc02729: SMc02729 - hypothetical protein, at 2,581,332 to 2,582,555
SMc02729
SMc02730: SMc02730 - hypothetical protein, at 2,582,559 to 2,583,848
SMc02730
Position (kb)
2581
2582
2583 Strain fitness (log2 ratio)
-2
-1
0
1 at 2580.345 kb on + strand, within SMc02728 at 2580.345 kb on + strand, within SMc02728 at 2580.352 kb on + strand, within SMc02728 at 2580.353 kb on - strand, within SMc02728 at 2580.353 kb on - strand, within SMc02728 at 2580.353 kb on - strand, within SMc02728 at 2580.353 kb on - strand, within SMc02728 at 2580.353 kb on - strand, within SMc02728 at 2580.496 kb on - strand, within SMc02728 at 2580.605 kb on + strand, within SMc02728 at 2580.788 kb on - strand, within SMc02728 at 2580.788 kb on - strand, within SMc02728 at 2580.788 kb on - strand, within SMc02728 at 2580.791 kb on + strand, within SMc02728 at 2580.791 kb on + strand, within SMc02728 at 2580.791 kb on + strand, within SMc02728 at 2580.791 kb on + strand, within SMc02728 at 2580.792 kb on - strand, within SMc02728 at 2580.821 kb on + strand, within SMc02728 at 2580.923 kb on + strand, within SMc02728 at 2581.230 kb on + strand at 2581.230 kb on + strand at 2581.230 kb on + strand at 2581.231 kb on - strand at 2581.314 kb on - strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.387 kb on + strand at 2581.388 kb on - strand at 2581.388 kb on - strand at 2581.388 kb on - strand at 2581.501 kb on + strand, within SMc02729 at 2581.502 kb on - strand, within SMc02729 at 2581.502 kb on - strand, within SMc02729 at 2581.570 kb on + strand, within SMc02729 at 2581.574 kb on - strand, within SMc02729 at 2581.579 kb on + strand, within SMc02729 at 2581.579 kb on + strand, within SMc02729 at 2581.580 kb on - strand, within SMc02729 at 2581.580 kb on - strand, within SMc02729 at 2581.791 kb on - strand, within SMc02729 at 2581.791 kb on - strand, within SMc02729 at 2581.802 kb on - strand, within SMc02729 at 2582.054 kb on - strand, within SMc02729 at 2582.187 kb on - strand, within SMc02729 at 2582.326 kb on + strand, within SMc02729 at 2582.326 kb on + strand, within SMc02729 at 2582.327 kb on - strand, within SMc02729 at 2582.371 kb on + strand, within SMc02729 at 2582.374 kb on - strand, within SMc02729 at 2582.377 kb on + strand, within SMc02729 at 2582.378 kb on - strand, within SMc02729 at 2582.378 kb on - strand, within SMc02729 at 2582.378 kb on - strand, within SMc02729 at 2582.520 kb on + strand at 2582.520 kb on + strand at 2582.560 kb on + strand at 2582.561 kb on - strand at 2582.614 kb on + strand at 2582.614 kb on + strand at 2582.615 kb on - strand at 2582.615 kb on - strand at 2582.615 kb on - strand at 2582.642 kb on - strand at 2582.642 kb on - strand at 2582.662 kb on + strand at 2582.671 kb on + strand at 2582.671 kb on + strand at 2582.671 kb on + strand at 2582.672 kb on - strand at 2582.672 kb on - strand at 2582.680 kb on + strand at 2582.878 kb on + strand, within SMc02730 at 2583.085 kb on + strand, within SMc02730 at 2583.085 kb on + strand, within SMc02730 at 2583.086 kb on - strand, within SMc02730 at 2583.115 kb on + strand, within SMc02730 at 2583.115 kb on + strand, within SMc02730 at 2583.115 kb on + strand, within SMc02730 at 2583.115 kb on + strand, within SMc02730 at 2583.115 kb on + strand, within SMc02730 at 2583.116 kb on - strand, within SMc02730 at 2583.279 kb on + strand, within SMc02730 at 2583.280 kb on - strand, within SMc02730
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 2,580,345 + SMc02728 0.47 -0.3 2,580,345 + SMc02728 0.47 -0.8 2,580,352 + SMc02728 0.47 +0.4 2,580,353 - SMc02728 0.47 -0.4 2,580,353 - SMc02728 0.47 -0.2 2,580,353 - SMc02728 0.47 -0.2 2,580,353 - SMc02728 0.47 -0.9 2,580,353 - SMc02728 0.47 -0.3 2,580,496 - SMc02728 0.56 +0.2 2,580,605 + SMc02728 0.62 +0.7 2,580,788 - SMc02728 0.73 +0.4 2,580,788 - SMc02728 0.73 -1.2 2,580,788 - SMc02728 0.73 -0.2 2,580,791 + SMc02728 0.73 -0.1 2,580,791 + SMc02728 0.73 -1.8 2,580,791 + SMc02728 0.73 +0.2 2,580,791 + SMc02728 0.73 +0.0 2,580,792 - SMc02728 0.73 -0.7 2,580,821 + SMc02728 0.75 +0.1 2,580,923 + SMc02728 0.81 -0.1 2,581,230 + -0.0 2,581,230 + -0.2 2,581,230 + -0.4 2,581,231 - +0.3 2,581,314 - +0.7 2,581,387 + +0.2 2,581,387 + +0.9 2,581,387 + -0.7 2,581,387 + -1.2 2,581,387 + +0.7 2,581,387 + -0.1 2,581,387 + -0.3 2,581,388 - -0.4 2,581,388 - +0.3 2,581,388 - +0.1 2,581,501 + SMc02729 0.14 -0.9 2,581,502 - SMc02729 0.14 -0.7 2,581,502 - SMc02729 0.14 -0.5 2,581,570 + SMc02729 0.19 -0.5 2,581,574 - SMc02729 0.20 -0.0 2,581,579 + SMc02729 0.20 -0.2 2,581,579 + SMc02729 0.20 -0.2 2,581,580 - SMc02729 0.20 -0.3 2,581,580 - SMc02729 0.20 -0.2 2,581,791 - SMc02729 0.38 -1.1 2,581,791 - SMc02729 0.38 -0.9 2,581,802 - SMc02729 0.38 -1.6 2,582,054 - SMc02729 0.59 -0.7 2,582,187 - SMc02729 0.70 -0.2 2,582,326 + SMc02729 0.81 +0.2 2,582,326 + SMc02729 0.81 +0.1 2,582,327 - SMc02729 0.81 -0.1 2,582,371 + SMc02729 0.85 -1.0 2,582,374 - SMc02729 0.85 -1.1 2,582,377 + SMc02729 0.85 -0.1 2,582,378 - SMc02729 0.85 -0.9 2,582,378 - SMc02729 0.85 +0.2 2,582,378 - SMc02729 0.85 -1.0 2,582,520 + -0.3 2,582,520 + +0.7 2,582,560 + -0.8 2,582,561 - -0.2 2,582,614 + -0.4 2,582,614 + -0.5 2,582,615 - -0.0 2,582,615 - -1.5 2,582,615 - +0.1 2,582,642 - -0.7 2,582,642 - -1.9 2,582,662 + +0.1 2,582,671 + -0.3 2,582,671 + -0.7 2,582,671 + +1.3 2,582,672 - -0.8 2,582,672 - +0.4 2,582,680 + -0.8 2,582,878 + SMc02730 0.25 +0.9 2,583,085 + SMc02730 0.41 -0.5 2,583,085 + SMc02730 0.41 +0.3 2,583,086 - SMc02730 0.41 +0.1 2,583,115 + SMc02730 0.43 -0.2 2,583,115 + SMc02730 0.43 +0.4 2,583,115 + SMc02730 0.43 +1.5 2,583,115 + SMc02730 0.43 -1.8 2,583,115 + SMc02730 0.43 +0.8 2,583,116 - SMc02730 0.43 -0.9 2,583,279 + SMc02730 0.56 -0.4 2,583,280 - SMc02730 0.56 -0.1
Or see this region's nucleotide sequence