Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02135 and SMc02134 are separated by 189 nucleotides SMc02134 and SMc02217 are separated by 35 nucleotides SMc02217 and SMc02218 are separated by 197 nucleotides
SMc02135: SMc02135 - ApaG protein, at 577,654 to 578,046
SMc02135
SMc02134: SMc02134 - hypothetical protein, at 578,236 to 578,847
SMc02134
SMc02217: SMc02217 - O-succinylhomoserine sulfhydrylase, at 578,883 to 580,067
SMc02217
SMc02218: SMc02218 - 2'-deoxycytidine 5'-triphosphate deaminase, at 580,265 to 581,362
SMc02218
Position (kb) 578
579
580
581 Strain fitness (log2 ratio) -2
-1
0
1 at 577.896 kb on - strand, within SMc02135 at 577.936 kb on + strand, within SMc02135 at 577.937 kb on - strand, within SMc02135 at 577.962 kb on - strand, within SMc02135 at 577.962 kb on - strand, within SMc02135 at 577.997 kb on + strand, within SMc02135 at 578.000 kb on + strand, within SMc02135 at 578.001 kb on - strand, within SMc02135 at 578.001 kb on - strand, within SMc02135 at 578.077 kb on + strand at 578.082 kb on + strand at 578.090 kb on + strand at 578.091 kb on - strand at 578.221 kb on + strand at 578.387 kb on - strand, within SMc02134 at 578.593 kb on + strand, within SMc02134 at 578.593 kb on + strand, within SMc02134 at 578.593 kb on + strand, within SMc02134 at 578.593 kb on + strand, within SMc02134 at 578.594 kb on - strand, within SMc02134 at 578.594 kb on - strand, within SMc02134 at 578.594 kb on - strand, within SMc02134 at 578.621 kb on - strand, within SMc02134 at 578.680 kb on + strand, within SMc02134 at 578.681 kb on - strand, within SMc02134 at 578.763 kb on - strand, within SMc02134 at 578.794 kb on + strand at 578.795 kb on - strand at 578.795 kb on - strand at 578.892 kb on + strand at 579.255 kb on + strand, within SMc02217 at 579.368 kb on - strand, within SMc02217 at 579.443 kb on + strand, within SMc02217 at 579.443 kb on + strand, within SMc02217 at 579.444 kb on - strand, within SMc02217 at 579.698 kb on - strand, within SMc02217 at 579.904 kb on + strand, within SMc02217 at 579.904 kb on + strand, within SMc02217 at 579.904 kb on + strand, within SMc02217 at 579.904 kb on + strand, within SMc02217 at 579.904 kb on + strand, within SMc02217 at 579.905 kb on - strand, within SMc02217 at 579.905 kb on - strand, within SMc02217 at 580.133 kb on + strand at 580.134 kb on - strand at 580.137 kb on + strand at 580.195 kb on + strand at 580.195 kb on + strand at 580.250 kb on + strand at 580.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 577,896 - SMc02135 0.62 -0.7 577,936 + SMc02135 0.72 +1.0 577,937 - SMc02135 0.72 +0.6 577,962 - SMc02135 0.78 +0.5 577,962 - SMc02135 0.78 -0.7 577,997 + SMc02135 0.87 -0.4 578,000 + SMc02135 0.88 -1.1 578,001 - SMc02135 0.88 +0.4 578,001 - SMc02135 0.88 -0.9 578,077 + -1.4 578,082 + -0.3 578,090 + +0.1 578,091 - -1.3 578,221 + -0.0 578,387 - SMc02134 0.25 +0.7 578,593 + SMc02134 0.58 -0.2 578,593 + SMc02134 0.58 -0.1 578,593 + SMc02134 0.58 +0.1 578,593 + SMc02134 0.58 +0.6 578,594 - SMc02134 0.58 +0.2 578,594 - SMc02134 0.58 -1.6 578,594 - SMc02134 0.58 +0.3 578,621 - SMc02134 0.63 +0.4 578,680 + SMc02134 0.73 +1.3 578,681 - SMc02134 0.73 -0.6 578,763 - SMc02134 0.86 +1.7 578,794 + +0.5 578,795 - +0.6 578,795 - -2.4 578,892 + +0.0 579,255 + SMc02217 0.31 -1.8 579,368 - SMc02217 0.41 +0.5 579,443 + SMc02217 0.47 +0.5 579,443 + SMc02217 0.47 +0.3 579,444 - SMc02217 0.47 +0.7 579,698 - SMc02217 0.69 +0.2 579,904 + SMc02217 0.86 +0.2 579,904 + SMc02217 0.86 +0.2 579,904 + SMc02217 0.86 +1.1 579,904 + SMc02217 0.86 +1.2 579,904 + SMc02217 0.86 -0.3 579,905 - SMc02217 0.86 -0.4 579,905 - SMc02217 0.86 +0.4 580,133 + +0.1 580,134 - +0.3 580,137 + +0.9 580,195 + -0.0 580,195 + -0.2 580,250 + -1.1 580,250 + +0.1
Or see this region's nucleotide sequence