Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00424 and SMc00425 are separated by 134 nucleotides SMc00425 and SMc00461 overlap by 8 nucleotides SMc00461 and SMc00427 are separated by 98 nucleotides
SMc00424: SMc00424 - hypothetical protein, at 372,883 to 373,797
SMc00424
SMc00425: SMc00425 - transcriptional regulator, at 373,932 to 374,369
SMc00425
SMc00461: SMc00461 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 374,362 to 374,844
SMc00461
SMc00427: SMc00427 - peptide chain release factor RF-3 protein, at 374,943 to 376,526
SMc00427
Position (kb) 374
375 Strain fitness (log2 ratio) -2
-1
0
1 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.497 kb on - strand, within SMc00424 at 373.497 kb on - strand, within SMc00424 at 373.746 kb on + strand at 373.747 kb on - strand at 373.783 kb on + strand at 373.783 kb on + strand at 373.784 kb on - strand at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.363 kb on + strand at 374.364 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.652 kb on + strand, within SMc00461 at 374.653 kb on - strand, within SMc00461 at 374.858 kb on + strand at 374.859 kb on - strand at 374.859 kb on - strand at 374.909 kb on + strand at 375.238 kb on - strand, within SMc00427 at 375.396 kb on + strand, within SMc00427 at 375.487 kb on - strand, within SMc00427 at 375.487 kb on - strand, within SMc00427
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 373,496 + SMc00424 0.67 +0.2 373,496 + SMc00424 0.67 +0.1 373,496 + SMc00424 0.67 -0.1 373,496 + SMc00424 0.67 +1.7 373,497 - SMc00424 0.67 -0.3 373,497 - SMc00424 0.67 -0.3 373,746 + -0.1 373,747 - +0.1 373,783 + -0.0 373,783 + +0.2 373,784 - -0.4 374,165 + SMc00425 0.53 -0.5 374,165 + SMc00425 0.53 -1.3 374,165 + SMc00425 0.53 +0.2 374,363 + -0.1 374,364 - +0.1 374,368 - -0.3 374,368 - +1.4 374,368 - -0.3 374,652 + SMc00461 0.60 -1.7 374,653 - SMc00461 0.60 -1.8 374,858 + -0.9 374,859 - +0.5 374,859 - +0.6 374,909 + +0.3 375,238 - SMc00427 0.19 +0.2 375,396 + SMc00427 0.29 -1.2 375,487 - SMc00427 0.34 +0.9 375,487 - SMc00427 0.34 -0.2
Or see this region's nucleotide sequence