Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0116 and SMa0117 overlap by 4 nucleotides SMa0117 and SMa0118 are separated by 226 nucleotides SMa0118 and SMa0121 are separated by 284 nucleotides SMa0121 and SMa0123 are separated by 70 nucleotides SMa0123 and SMa0124 are separated by 79 nucleotides
SMa0116: SMa0116 - DnaJ/CbpA-type protein, at 63,200 to 64,117
SMa0116
SMa0117: SMa0117 - hypothetical protein, at 64,114 to 64,452
SMa0117
SMa0118: SMa0118 - hypothetical protein, at 64,679 to 65,206
SMa0118
SMa0121: SMa0121 - hypothetical protein, at 65,491 to 65,658
SMa0121
SMa0123: SMa0123 - hypothetical protein, at 65,729 to 66,112
SMa0123
SMa0124: SMa0124 - molecular chaperone GroEL, at 66,192 to 67,826
SMa0124
Position (kb) 64
65
66 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 63.781 kb on + strand, within SMa0116 at 63.781 kb on + strand, within SMa0116 at 63.782 kb on - strand, within SMa0116 at 63.831 kb on + strand, within SMa0116 at 63.831 kb on + strand, within SMa0116 at 63.831 kb on + strand, within SMa0116 at 63.832 kb on - strand, within SMa0116 at 63.832 kb on - strand, within SMa0116 at 63.844 kb on + strand, within SMa0116 at 63.851 kb on + strand, within SMa0116 at 63.919 kb on + strand, within SMa0116 at 64.082 kb on + strand at 64.157 kb on + strand, within SMa0117 at 64.158 kb on - strand, within SMa0117 at 64.359 kb on + strand, within SMa0117 at 64.400 kb on + strand, within SMa0117 at 64.401 kb on - strand, within SMa0117 at 64.450 kb on + strand at 64.450 kb on + strand at 64.450 kb on + strand at 64.450 kb on + strand at 64.451 kb on - strand at 64.451 kb on - strand at 64.451 kb on - strand at 64.451 kb on - strand at 64.454 kb on + strand at 64.454 kb on + strand at 64.455 kb on - strand at 64.455 kb on - strand at 64.455 kb on - strand at 64.464 kb on + strand at 64.464 kb on + strand at 64.464 kb on + strand at 64.616 kb on + strand at 64.616 kb on + strand at 64.616 kb on + strand at 64.616 kb on + strand at 64.617 kb on - strand at 64.617 kb on - strand at 64.617 kb on - strand at 64.617 kb on - strand at 64.619 kb on - strand at 64.635 kb on - strand at 64.674 kb on + strand at 64.674 kb on + strand at 64.674 kb on + strand at 64.674 kb on + strand at 64.675 kb on - strand at 64.675 kb on - strand at 64.782 kb on - strand, within SMa0118 at 64.876 kb on - strand, within SMa0118 at 64.876 kb on - strand, within SMa0118 at 65.145 kb on + strand, within SMa0118 at 65.145 kb on + strand, within SMa0118 at 65.156 kb on - strand at 65.156 kb on - strand at 65.331 kb on - strand at 65.484 kb on + strand at 65.484 kb on + strand at 65.484 kb on + strand at 65.484 kb on + strand at 65.485 kb on - strand at 65.485 kb on - strand at 65.485 kb on - strand at 65.585 kb on - strand, within SMa0121 at 65.595 kb on + strand, within SMa0121 at 65.596 kb on - strand, within SMa0121 at 65.835 kb on + strand, within SMa0123 at 65.835 kb on + strand, within SMa0123 at 66.044 kb on + strand, within SMa0123 at 66.045 kb on - strand, within SMa0123 at 66.069 kb on + strand, within SMa0123 at 66.069 kb on + strand, within SMa0123 at 66.197 kb on - strand at 66.203 kb on + strand at 66.204 kb on - strand at 66.204 kb on - strand at 66.204 kb on - strand at 66.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 63,781 + SMa0116 0.63 -0.1 63,781 + SMa0116 0.63 +0.2 63,782 - SMa0116 0.63 +0.9 63,831 + SMa0116 0.69 -0.1 63,831 + SMa0116 0.69 -0.3 63,831 + SMa0116 0.69 +0.4 63,832 - SMa0116 0.69 +0.6 63,832 - SMa0116 0.69 +0.1 63,844 + SMa0116 0.70 -1.2 63,851 + SMa0116 0.71 -0.5 63,919 + SMa0116 0.78 -1.0 64,082 + -0.8 64,157 + SMa0117 0.13 -0.3 64,158 - SMa0117 0.13 +0.0 64,359 + SMa0117 0.72 -0.4 64,400 + SMa0117 0.84 +0.1 64,401 - SMa0117 0.85 +0.9 64,450 + +0.2 64,450 + +1.3 64,450 + +0.3 64,450 + +0.2 64,451 - -0.2 64,451 - +0.1 64,451 - +0.1 64,451 - +0.4 64,454 + -0.2 64,454 + +2.5 64,455 - -0.0 64,455 - -2.8 64,455 - +0.5 64,464 + -1.8 64,464 + -0.1 64,464 + +0.4 64,616 + -1.0 64,616 + -0.4 64,616 + -0.0 64,616 + -0.1 64,617 - -0.3 64,617 - -0.1 64,617 - -0.2 64,617 - -0.8 64,619 - -0.3 64,635 - +1.2 64,674 + +0.9 64,674 + -1.2 64,674 + -0.2 64,674 + -1.0 64,675 - +0.0 64,675 - +0.1 64,782 - SMa0118 0.20 -0.3 64,876 - SMa0118 0.37 -1.4 64,876 - SMa0118 0.37 -0.2 65,145 + SMa0118 0.88 +0.4 65,145 + SMa0118 0.88 -0.2 65,156 - +0.5 65,156 - +0.0 65,331 - -1.6 65,484 + -0.7 65,484 + -0.0 65,484 + +0.1 65,484 + -0.7 65,485 - -0.2 65,485 - -0.3 65,485 - -0.6 65,585 - SMa0121 0.56 +0.0 65,595 + SMa0121 0.62 -0.4 65,596 - SMa0121 0.62 +0.4 65,835 + SMa0123 0.28 -1.3 65,835 + SMa0123 0.28 -0.3 66,044 + SMa0123 0.82 +0.1 66,045 - SMa0123 0.82 +0.4 66,069 + SMa0123 0.89 +0.6 66,069 + SMa0123 0.89 -0.9 66,197 - -0.6 66,203 + -0.1 66,204 - -0.2 66,204 - -0.4 66,204 - -0.0 66,204 - -0.6
Or see this region's nucleotide sequence