Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21348 and SM_b21349 overlap by 4 nucleotides SM_b21349 and SM_b21350 are separated by 132 nucleotides SM_b21350 and SM_b21351 are separated by 8 nucleotides
SM_b21348: SM_b21348 - 2-deoxy-D-gluconate 3-dehydrogenase, at 1,014,047 to 1,014,811
_b21348
SM_b21349: SM_b21349 - 5-keto-4-deoxyuronate isomerase, at 1,014,808 to 1,015,656
_b21349
SM_b21350: SM_b21350 - GntR family transcriptional regulator, at 1,015,789 to 1,016,538
_b21350
SM_b21351: SM_b21351 - C4-dicarboxylate large membrane transport protein, at 1,016,547 to 1,017,830
_b21351
Position (kb)
1015
1016
1017 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1014.847 kb on - strand at 1014.847 kb on - strand at 1014.848 kb on + strand at 1014.849 kb on - strand at 1014.855 kb on + strand at 1014.856 kb on - strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.887 kb on + strand at 1014.888 kb on - strand at 1014.955 kb on + strand, within SM_b21349 at 1014.955 kb on + strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1014.956 kb on - strand, within SM_b21349 at 1015.025 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.031 kb on + strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.032 kb on - strand, within SM_b21349 at 1015.222 kb on - strand, within SM_b21349 at 1015.265 kb on + strand, within SM_b21349 at 1015.328 kb on + strand, within SM_b21349 at 1015.329 kb on - strand, within SM_b21349 at 1015.437 kb on + strand, within SM_b21349 at 1015.518 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.520 kb on + strand, within SM_b21349 at 1015.521 kb on - strand, within SM_b21349 at 1015.634 kb on + strand at 1015.696 kb on + strand at 1015.696 kb on + strand at 1015.697 kb on - strand at 1015.697 kb on - strand at 1015.700 kb on + strand at 1015.701 kb on - strand at 1015.701 kb on - strand at 1015.724 kb on + strand at 1015.725 kb on - strand at 1015.759 kb on - strand at 1016.051 kb on + strand, within SM_b21350 at 1016.052 kb on - strand, within SM_b21350 at 1016.052 kb on - strand, within SM_b21350 at 1016.054 kb on + strand, within SM_b21350 at 1016.181 kb on + strand, within SM_b21350 at 1016.455 kb on + strand, within SM_b21350 at 1016.455 kb on + strand, within SM_b21350 at 1016.456 kb on - strand, within SM_b21350 at 1016.575 kb on - strand at 1016.593 kb on + strand at 1016.594 kb on - strand at 1016.594 kb on - strand at 1016.594 kb on - strand at 1016.640 kb on + strand at 1016.640 kb on + strand at 1016.641 kb on - strand at 1016.641 kb on - strand at 1016.739 kb on + strand, within SM_b21351 at 1016.739 kb on + strand, within SM_b21351 at 1016.779 kb on + strand, within SM_b21351 at 1016.878 kb on - strand, within SM_b21351 at 1016.878 kb on - strand, within SM_b21351 at 1016.883 kb on + strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1016.884 kb on - strand, within SM_b21351 at 1017.088 kb on + strand, within SM_b21351 at 1017.088 kb on + strand, within SM_b21351 at 1017.089 kb on - strand, within SM_b21351 at 1017.138 kb on + strand, within SM_b21351 at 1017.138 kb on + strand, within SM_b21351 at 1017.194 kb on - strand, within SM_b21351 at 1017.194 kb on - strand, within SM_b21351 at 1017.259 kb on + strand, within SM_b21351 at 1017.286 kb on + strand, within SM_b21351 at 1017.359 kb on - strand, within SM_b21351 at 1017.359 kb on - strand, within SM_b21351 at 1017.443 kb on - strand, within SM_b21351 at 1017.480 kb on + strand, within SM_b21351 at 1017.481 kb on - strand, within SM_b21351
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 1,014,847 - +1.0 1,014,847 - -0.7 1,014,848 + +0.9 1,014,849 - +0.0 1,014,855 + +1.5 1,014,856 - +1.1 1,014,887 + +0.2 1,014,887 + +0.1 1,014,887 + +0.1 1,014,888 - +0.5 1,014,955 + SM_b21349 0.17 +1.0 1,014,955 + SM_b21349 0.17 -1.2 1,014,956 - SM_b21349 0.17 +0.2 1,014,956 - SM_b21349 0.17 +0.7 1,015,025 + SM_b21349 0.26 -0.5 1,015,031 + SM_b21349 0.26 +0.3 1,015,031 + SM_b21349 0.26 +0.0 1,015,032 - SM_b21349 0.26 +0.4 1,015,032 - SM_b21349 0.26 +1.0 1,015,222 - SM_b21349 0.49 -0.2 1,015,265 + SM_b21349 0.54 -0.1 1,015,328 + SM_b21349 0.61 +1.2 1,015,329 - SM_b21349 0.61 -0.1 1,015,437 + SM_b21349 0.74 +0.0 1,015,518 + SM_b21349 0.84 -1.2 1,015,520 + SM_b21349 0.84 -0.6 1,015,520 + SM_b21349 0.84 -1.0 1,015,520 + SM_b21349 0.84 -0.7 1,015,521 - SM_b21349 0.84 +0.7 1,015,634 + +0.7 1,015,696 + -0.5 1,015,696 + +0.4 1,015,697 - +2.2 1,015,697 - +0.7 1,015,700 + +0.4 1,015,701 - -0.2 1,015,701 - -0.5 1,015,724 + -0.8 1,015,725 - +0.2 1,015,759 - -0.2 1,016,051 + SM_b21350 0.35 -1.6 1,016,052 - SM_b21350 0.35 +0.1 1,016,052 - SM_b21350 0.35 -0.4 1,016,054 + SM_b21350 0.35 -0.3 1,016,181 + SM_b21350 0.52 -0.8 1,016,455 + SM_b21350 0.89 +0.4 1,016,455 + SM_b21350 0.89 -1.5 1,016,456 - SM_b21350 0.89 +0.4 1,016,575 - -0.7 1,016,593 + -0.5 1,016,594 - -1.5 1,016,594 - +0.1 1,016,594 - +0.4 1,016,640 + +0.6 1,016,640 + +1.8 1,016,641 - +0.4 1,016,641 - +0.4 1,016,739 + SM_b21351 0.15 +1.6 1,016,739 + SM_b21351 0.15 -0.3 1,016,779 + SM_b21351 0.18 +0.9 1,016,878 - SM_b21351 0.26 -1.9 1,016,878 - SM_b21351 0.26 +0.7 1,016,883 + SM_b21351 0.26 +0.2 1,016,884 - SM_b21351 0.26 +1.2 1,016,884 - SM_b21351 0.26 +0.6 1,016,884 - SM_b21351 0.26 -1.1 1,017,088 + SM_b21351 0.42 -0.1 1,017,088 + SM_b21351 0.42 +0.7 1,017,089 - SM_b21351 0.42 -0.1 1,017,138 + SM_b21351 0.46 +0.1 1,017,138 + SM_b21351 0.46 -0.9 1,017,194 - SM_b21351 0.50 +0.1 1,017,194 - SM_b21351 0.50 +0.4 1,017,259 + SM_b21351 0.55 -0.3 1,017,286 + SM_b21351 0.58 +0.5 1,017,359 - SM_b21351 0.63 -0.2 1,017,359 - SM_b21351 0.63 +1.2 1,017,443 - SM_b21351 0.70 +1.2 1,017,480 + SM_b21351 0.73 +0.2 1,017,481 - SM_b21351 0.73 -0.1
Or see this region's nucleotide sequence