Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21185 and SM_b21186 are separated by 115 nucleotides SM_b21186 and SM_b21187 are separated by 212 nucleotides
SM_b21185: SM_b21185 - succinate-semialdehyde dehydrogenase (NAD(P)+) protein, at 933,762 to 935,237
_b21185
SM_b21186: SM_b21186 - 4-aminobutyrate aminotransferase, at 935,353 to 936,621
_b21186
SM_b21187: SM_b21187 - LacI family transcriptional regulator, at 936,834 to 937,664
_b21187
Position (kb) 935
936
937 Strain fitness (log2 ratio) -2
-1
0
1 at 934.588 kb on + strand, within SM_b21185 at 934.589 kb on - strand, within SM_b21185 at 934.770 kb on + strand, within SM_b21185 at 934.800 kb on - strand, within SM_b21185 at 934.800 kb on - strand, within SM_b21185 at 934.873 kb on + strand, within SM_b21185 at 934.873 kb on + strand, within SM_b21185 at 934.874 kb on - strand, within SM_b21185 at 934.874 kb on - strand, within SM_b21185 at 935.103 kb on + strand at 935.104 kb on - strand at 935.283 kb on + strand at 935.283 kb on + strand at 935.284 kb on - strand at 935.284 kb on - strand at 935.385 kb on + strand at 935.739 kb on - strand, within SM_b21186 at 935.739 kb on - strand, within SM_b21186 at 935.739 kb on - strand, within SM_b21186 at 935.987 kb on + strand, within SM_b21186 at 936.168 kb on - strand, within SM_b21186 at 936.168 kb on - strand, within SM_b21186 at 936.168 kb on - strand, within SM_b21186 at 936.375 kb on - strand, within SM_b21186 at 936.375 kb on - strand, within SM_b21186 at 936.405 kb on + strand, within SM_b21186 at 936.405 kb on + strand, within SM_b21186 at 936.468 kb on + strand, within SM_b21186 at 936.468 kb on + strand, within SM_b21186 at 936.468 kb on + strand, within SM_b21186 at 936.469 kb on - strand, within SM_b21186 at 936.503 kb on + strand at 936.504 kb on - strand at 936.504 kb on - strand at 936.647 kb on + strand at 936.685 kb on + strand at 936.779 kb on - strand at 936.783 kb on - strand at 936.962 kb on + strand, within SM_b21187 at 936.963 kb on - strand, within SM_b21187 at 936.970 kb on - strand, within SM_b21187 at 936.970 kb on - strand, within SM_b21187 at 936.970 kb on - strand, within SM_b21187 at 936.970 kb on - strand, within SM_b21187 at 936.970 kb on - strand, within SM_b21187 at 936.971 kb on + strand, within SM_b21187 at 936.971 kb on + strand, within SM_b21187 at 936.971 kb on + strand, within SM_b21187 at 936.971 kb on + strand, within SM_b21187 at 936.971 kb on + strand, within SM_b21187 at 936.972 kb on - strand, within SM_b21187 at 937.548 kb on + strand, within SM_b21187 at 937.548 kb on + strand, within SM_b21187
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 934,588 + SM_b21185 0.56 +0.7 934,589 - SM_b21185 0.56 -0.1 934,770 + SM_b21185 0.68 -0.0 934,800 - SM_b21185 0.70 +1.6 934,800 - SM_b21185 0.70 +0.2 934,873 + SM_b21185 0.75 +0.8 934,873 + SM_b21185 0.75 +0.3 934,874 - SM_b21185 0.75 -0.1 934,874 - SM_b21185 0.75 +0.4 935,103 + -0.3 935,104 - -0.7 935,283 + -0.2 935,283 + +0.8 935,284 - -0.1 935,284 - +0.8 935,385 + +0.2 935,739 - SM_b21186 0.30 -0.6 935,739 - SM_b21186 0.30 -1.2 935,739 - SM_b21186 0.30 +0.0 935,987 + SM_b21186 0.50 +0.2 936,168 - SM_b21186 0.64 +0.2 936,168 - SM_b21186 0.64 -0.2 936,168 - SM_b21186 0.64 -0.0 936,375 - SM_b21186 0.81 -0.4 936,375 - SM_b21186 0.81 -1.2 936,405 + SM_b21186 0.83 +1.1 936,405 + SM_b21186 0.83 -1.2 936,468 + SM_b21186 0.88 +0.1 936,468 + SM_b21186 0.88 +1.4 936,468 + SM_b21186 0.88 -0.5 936,469 - SM_b21186 0.88 -0.3 936,503 + +0.7 936,504 - -0.3 936,504 - -2.7 936,647 + -0.0 936,685 + -0.2 936,779 - -0.1 936,783 - -0.4 936,962 + SM_b21187 0.15 -1.0 936,963 - SM_b21187 0.16 -0.5 936,970 - SM_b21187 0.16 -1.2 936,970 - SM_b21187 0.16 -0.1 936,970 - SM_b21187 0.16 -0.4 936,970 - SM_b21187 0.16 +0.2 936,970 - SM_b21187 0.16 -0.1 936,971 + SM_b21187 0.16 -0.6 936,971 + SM_b21187 0.16 -0.5 936,971 + SM_b21187 0.16 -0.4 936,971 + SM_b21187 0.16 +0.3 936,971 + SM_b21187 0.16 -0.8 936,972 - SM_b21187 0.17 -0.5 937,548 + SM_b21187 0.86 +0.1 937,548 + SM_b21187 0.86 +0.4
Or see this region's nucleotide sequence