Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20211 and SM_b20212 are separated by 187 nucleotides SM_b20212 and SM_b20213 are separated by 37 nucleotides SM_b20213 and SM_b20214 are separated by 263 nucleotides
SM_b20211: SM_b20211 - transcriptional regulator, at 218,142 to 219,074
_b20211
SM_b20212: SM_b20212 - hypothetical protein, at 219,262 to 219,942
_b20212
SM_b20213: SM_b20213 - hypothetical protein, at 219,980 to 221,863
_b20213
SM_b20214: SM_b20214 - oxidoreductase, at 222,127 to 222,930
_b20214
Position (kb) 219
220
221
222 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 219.074 kb on + strand at 219.190 kb on - strand at 219.191 kb on + strand at 219.210 kb on + strand at 219.219 kb on + strand at 219.359 kb on - strand, within SM_b20212 at 219.361 kb on + strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.362 kb on - strand, within SM_b20212 at 219.514 kb on - strand, within SM_b20212 at 219.524 kb on + strand, within SM_b20212 at 219.524 kb on + strand, within SM_b20212 at 219.525 kb on - strand, within SM_b20212 at 219.562 kb on + strand, within SM_b20212 at 219.563 kb on - strand, within SM_b20212 at 219.634 kb on + strand, within SM_b20212 at 219.635 kb on - strand, within SM_b20212 at 219.826 kb on + strand, within SM_b20212 at 219.940 kb on + strand at 219.971 kb on - strand at 220.131 kb on + strand at 220.132 kb on - strand at 220.136 kb on + strand at 220.136 kb on + strand at 220.136 kb on + strand at 220.598 kb on + strand, within SM_b20213 at 220.598 kb on + strand, within SM_b20213 at 220.599 kb on - strand, within SM_b20213 at 220.781 kb on + strand, within SM_b20213 at 221.009 kb on + strand, within SM_b20213 at 221.009 kb on + strand, within SM_b20213 at 221.009 kb on + strand, within SM_b20213 at 221.032 kb on + strand, within SM_b20213 at 221.043 kb on - strand, within SM_b20213 at 221.043 kb on - strand, within SM_b20213 at 221.066 kb on + strand, within SM_b20213 at 221.066 kb on + strand, within SM_b20213 at 221.120 kb on + strand, within SM_b20213 at 221.225 kb on + strand, within SM_b20213 at 221.226 kb on - strand, within SM_b20213 at 221.226 kb on - strand, within SM_b20213 at 221.241 kb on - strand, within SM_b20213 at 221.316 kb on + strand, within SM_b20213 at 221.316 kb on + strand, within SM_b20213 at 221.435 kb on + strand, within SM_b20213 at 221.435 kb on + strand, within SM_b20213 at 221.435 kb on + strand, within SM_b20213 at 221.436 kb on - strand, within SM_b20213 at 221.445 kb on - strand, within SM_b20213 at 221.504 kb on + strand, within SM_b20213 at 221.504 kb on + strand, within SM_b20213 at 221.504 kb on + strand, within SM_b20213 at 221.766 kb on - strand at 221.776 kb on - strand at 221.902 kb on + strand at 222.034 kb on + strand at 222.091 kb on - strand at 222.191 kb on - strand at 222.276 kb on + strand, within SM_b20214 at 222.359 kb on + strand, within SM_b20214 at 222.360 kb on - strand, within SM_b20214 at 222.493 kb on + strand, within SM_b20214 at 222.494 kb on - strand, within SM_b20214 at 222.516 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.544 kb on - strand, within SM_b20214 at 222.589 kb on + strand, within SM_b20214 at 222.666 kb on - strand, within SM_b20214 at 222.666 kb on - strand, within SM_b20214 at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.850 kb on + strand at 222.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 219,074 + -0.3 219,190 - -1.2 219,191 + +0.4 219,210 + -0.2 219,219 + -0.5 219,359 - SM_b20212 0.14 +0.1 219,361 + SM_b20212 0.15 +0.2 219,362 - SM_b20212 0.15 -0.6 219,362 - SM_b20212 0.15 -0.4 219,362 - SM_b20212 0.15 +0.1 219,514 - SM_b20212 0.37 -0.6 219,524 + SM_b20212 0.38 +0.1 219,524 + SM_b20212 0.38 -0.0 219,525 - SM_b20212 0.39 -0.3 219,562 + SM_b20212 0.44 -0.0 219,563 - SM_b20212 0.44 -0.1 219,634 + SM_b20212 0.55 +0.2 219,635 - SM_b20212 0.55 +0.2 219,826 + SM_b20212 0.83 -0.1 219,940 + -0.2 219,971 - -0.5 220,131 + +0.1 220,132 - -1.2 220,136 + -0.5 220,136 + +1.3 220,136 + -1.1 220,598 + SM_b20213 0.33 -0.5 220,598 + SM_b20213 0.33 +0.1 220,599 - SM_b20213 0.33 -0.5 220,781 + SM_b20213 0.43 +0.4 221,009 + SM_b20213 0.55 -0.5 221,009 + SM_b20213 0.55 -0.5 221,009 + SM_b20213 0.55 -0.6 221,032 + SM_b20213 0.56 -0.5 221,043 - SM_b20213 0.56 -1.0 221,043 - SM_b20213 0.56 +0.2 221,066 + SM_b20213 0.58 +0.2 221,066 + SM_b20213 0.58 -0.6 221,120 + SM_b20213 0.61 +0.4 221,225 + SM_b20213 0.66 -0.1 221,226 - SM_b20213 0.66 -0.2 221,226 - SM_b20213 0.66 +0.4 221,241 - SM_b20213 0.67 +0.2 221,316 + SM_b20213 0.71 -0.3 221,316 + SM_b20213 0.71 -0.4 221,435 + SM_b20213 0.77 -0.0 221,435 + SM_b20213 0.77 -0.4 221,435 + SM_b20213 0.77 -1.1 221,436 - SM_b20213 0.77 -0.4 221,445 - SM_b20213 0.78 -1.1 221,504 + SM_b20213 0.81 -0.2 221,504 + SM_b20213 0.81 -0.0 221,504 + SM_b20213 0.81 -0.7 221,766 - +0.1 221,776 - +0.3 221,902 + +0.5 222,034 + -0.8 222,091 - -0.2 222,191 - -0.1 222,276 + SM_b20214 0.19 -0.5 222,359 + SM_b20214 0.29 -0.0 222,360 - SM_b20214 0.29 -0.8 222,493 + SM_b20214 0.46 -2.6 222,494 - SM_b20214 0.46 +0.4 222,516 - SM_b20214 0.48 -0.7 222,544 - SM_b20214 0.52 -0.8 222,544 - SM_b20214 0.52 -1.8 222,544 - SM_b20214 0.52 -0.8 222,589 + SM_b20214 0.57 -0.7 222,666 - SM_b20214 0.67 +1.5 222,666 - SM_b20214 0.67 -0.2 222,850 + +0.4 222,850 + +0.1 222,850 + +1.5 222,850 + -0.7 222,850 + +4.4 222,851 - -1.0
Or see this region's nucleotide sequence