Experiment: LB, pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20042 and SM_b20043 are separated by 373 nucleotides SM_b20043 and SM_b20044 are separated by 460 nucleotides
SM_b20042: SM_b20042 - hypothetical protein, at 52,256 to 53,413
_b20042
SM_b20043: SM_b20043 - hypothetical protein, at 53,787 to 54,629
_b20043
SM_b20044: SM_b20044 - replication initiation protein RepC, at 55,090 to 56,400
_b20044
Position (kb) 53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 53.213 kb on + strand, within SM_b20042 at 53.407 kb on - strand at 53.475 kb on + strand at 53.476 kb on - strand at 53.496 kb on + strand at 53.496 kb on + strand at 53.497 kb on - strand at 53.568 kb on + strand at 53.579 kb on + strand at 53.579 kb on + strand at 53.830 kb on + strand at 53.830 kb on + strand at 53.830 kb on + strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.831 kb on - strand at 53.837 kb on + strand at 53.840 kb on - strand at 53.857 kb on - strand at 53.942 kb on + strand, within SM_b20043 at 53.942 kb on + strand, within SM_b20043 at 53.943 kb on - strand, within SM_b20043 at 54.014 kb on + strand, within SM_b20043 at 54.015 kb on - strand, within SM_b20043 at 54.015 kb on - strand, within SM_b20043 at 54.033 kb on - strand, within SM_b20043 at 54.177 kb on - strand, within SM_b20043 at 54.208 kb on + strand, within SM_b20043 at 54.232 kb on + strand, within SM_b20043 at 54.232 kb on + strand, within SM_b20043 at 54.286 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.400 kb on - strand, within SM_b20043 at 54.440 kb on + strand, within SM_b20043 at 54.476 kb on - strand, within SM_b20043 at 54.525 kb on - strand, within SM_b20043 at 54.585 kb on + strand at 54.586 kb on - strand at 54.588 kb on - strand at 54.643 kb on - strand at 54.775 kb on + strand at 54.971 kb on - strand at 55.066 kb on + strand at 55.066 kb on + strand at 55.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH8 remove 53,213 + SM_b20042 0.83 +0.1 53,407 - +0.3 53,475 + -1.9 53,476 - +0.0 53,496 + -0.3 53,496 + +0.0 53,497 - +0.2 53,568 + -0.4 53,579 + +1.0 53,579 + -0.0 53,830 + -1.4 53,830 + -1.2 53,830 + -0.4 53,831 - -0.3 53,831 - -1.3 53,831 - -0.5 53,831 - -1.6 53,837 + -0.7 53,840 - -0.6 53,857 - -0.8 53,942 + SM_b20043 0.18 -0.5 53,942 + SM_b20043 0.18 -0.5 53,943 - SM_b20043 0.19 -1.0 54,014 + SM_b20043 0.27 -1.3 54,015 - SM_b20043 0.27 -0.7 54,015 - SM_b20043 0.27 -1.7 54,033 - SM_b20043 0.29 -0.6 54,177 - SM_b20043 0.46 -2.3 54,208 + SM_b20043 0.50 -0.2 54,232 + SM_b20043 0.53 -2.7 54,232 + SM_b20043 0.53 -1.4 54,286 - SM_b20043 0.59 +0.0 54,400 - SM_b20043 0.73 -2.2 54,400 - SM_b20043 0.73 -0.6 54,400 - SM_b20043 0.73 -1.0 54,440 + SM_b20043 0.77 -0.9 54,476 - SM_b20043 0.82 -0.4 54,525 - SM_b20043 0.88 -0.8 54,585 + -0.3 54,586 - -0.6 54,588 - +0.0 54,643 - +0.1 54,775 + +3.1 54,971 - +0.2 55,066 + -0.8 55,066 + -0.4 55,066 + -0.6
Or see this region's nucleotide sequence