Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25655

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25640 and RR42_RS25645 are separated by 115 nucleotidesRR42_RS25645 and RR42_RS25650 are separated by 316 nucleotidesRR42_RS25650 and RR42_RS25655 are separated by 95 nucleotidesRR42_RS25655 and RR42_RS25660 are separated by 8 nucleotides RR42_RS25640: RR42_RS25640 - 3-hydroxyisobutyrate dehydrogenase, at 1,096,083 to 1,096,979 _RS25640 RR42_RS25645: RR42_RS25645 - hypothetical protein, at 1,097,095 to 1,097,289 _RS25645 RR42_RS25650: RR42_RS25650 - hypothetical protein, at 1,097,606 to 1,097,806 _RS25650 RR42_RS25655: RR42_RS25655 - pyruvate carboxylase, at 1,097,902 to 1,101,411 _RS25655 RR42_RS25660: RR42_RS25660 - cytochrome C biogenesis protein, at 1,101,420 to 1,102,091 _RS25660 Position (kb) 1097 1098 1099 1100 1101 1102Strain fitness (log2 ratio) -2 -1 0 1 2at 1097.089 kb on + strandat 1097.089 kb on + strandat 1097.089 kb on + strandat 1097.097 kb on - strandat 1097.250 kb on + strand, within RR42_RS25645at 1097.250 kb on + strand, within RR42_RS25645at 1097.251 kb on - strand, within RR42_RS25645at 1097.251 kb on - strand, within RR42_RS25645at 1097.294 kb on + strandat 1097.295 kb on - strandat 1097.295 kb on - strandat 1097.295 kb on - strandat 1097.338 kb on + strandat 1097.338 kb on + strandat 1097.338 kb on + strandat 1097.339 kb on - strandat 1097.339 kb on - strandat 1097.460 kb on - strandat 1097.610 kb on - strandat 1097.610 kb on - strandat 1097.610 kb on - strandat 1097.613 kb on - strandat 1097.613 kb on - strandat 1097.704 kb on + strand, within RR42_RS25650at 1097.704 kb on + strand, within RR42_RS25650at 1097.798 kb on + strandat 1097.799 kb on - strandat 1097.799 kb on - strandat 1097.901 kb on + strandat 1097.908 kb on + strandat 1097.908 kb on + strandat 1097.989 kb on - strandat 1098.000 kb on - strandat 1098.007 kb on + strandat 1098.059 kb on - strandat 1098.095 kb on - strandat 1098.508 kb on + strand, within RR42_RS25655at 1098.508 kb on + strand, within RR42_RS25655at 1098.508 kb on + strand, within RR42_RS25655at 1098.509 kb on - strand, within RR42_RS25655at 1098.509 kb on - strand, within RR42_RS25655at 1098.589 kb on + strand, within RR42_RS25655at 1098.589 kb on + strand, within RR42_RS25655at 1098.590 kb on - strand, within RR42_RS25655at 1098.590 kb on - strand, within RR42_RS25655at 1098.603 kb on + strand, within RR42_RS25655at 1098.604 kb on - strand, within RR42_RS25655at 1098.604 kb on - strand, within RR42_RS25655at 1098.772 kb on + strand, within RR42_RS25655at 1098.772 kb on + strand, within RR42_RS25655at 1098.773 kb on - strand, within RR42_RS25655at 1098.810 kb on + strand, within RR42_RS25655at 1098.811 kb on - strand, within RR42_RS25655at 1098.811 kb on - strand, within RR42_RS25655at 1099.030 kb on + strand, within RR42_RS25655at 1099.123 kb on + strand, within RR42_RS25655at 1099.366 kb on + strand, within RR42_RS25655at 1099.624 kb on - strand, within RR42_RS25655at 1099.825 kb on + strand, within RR42_RS25655at 1099.825 kb on + strand, within RR42_RS25655at 1099.826 kb on - strand, within RR42_RS25655at 1100.018 kb on - strand, within RR42_RS25655at 1100.018 kb on - strand, within RR42_RS25655at 1100.029 kb on + strand, within RR42_RS25655at 1100.029 kb on + strand, within RR42_RS25655at 1100.030 kb on - strand, within RR42_RS25655at 1100.030 kb on - strand, within RR42_RS25655at 1100.032 kb on + strand, within RR42_RS25655at 1100.033 kb on - strand, within RR42_RS25655at 1100.287 kb on - strand, within RR42_RS25655at 1100.372 kb on - strand, within RR42_RS25655at 1100.372 kb on - strand, within RR42_RS25655at 1100.372 kb on - strand, within RR42_RS25655at 1100.372 kb on - strand, within RR42_RS25655at 1100.384 kb on + strand, within RR42_RS25655at 1100.393 kb on - strand, within RR42_RS25655at 1100.395 kb on + strand, within RR42_RS25655at 1100.395 kb on + strand, within RR42_RS25655at 1100.536 kb on + strand, within RR42_RS25655at 1100.537 kb on - strand, within RR42_RS25655at 1100.894 kb on - strand, within RR42_RS25655at 1100.936 kb on - strand, within RR42_RS25655at 1100.938 kb on - strand, within RR42_RS25655at 1100.938 kb on - strand, within RR42_RS25655at 1100.943 kb on + strand, within RR42_RS25655at 1101.352 kb on - strandat 1101.456 kb on + strandat 1101.457 kb on - strandat 1101.642 kb on - strand, within RR42_RS25660at 1101.663 kb on + strand, within RR42_RS25660at 1101.664 kb on - strand, within RR42_RS25660at 1101.673 kb on - strand, within RR42_RS25660at 1101.732 kb on + strand, within RR42_RS25660at 1101.994 kb on - strand, within RR42_RS25660at 1102.072 kb on - strandat 1102.143 kb on + strandat 1102.144 kb on - strandat 1102.388 kb on + strandat 1102.389 kb on - strandat 1102.389 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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1,097,089 + +2.0
1,097,089 + +0.1
1,097,089 + +0.1
1,097,097 - +1.4
1,097,250 + RR42_RS25645 0.79 -0.7
1,097,250 + RR42_RS25645 0.79 +0.6
1,097,251 - RR42_RS25645 0.80 -1.5
1,097,251 - RR42_RS25645 0.80 -0.9
1,097,294 + -0.4
1,097,295 - +0.5
1,097,295 - +0.4
1,097,295 - +0.5
1,097,338 + +0.2
1,097,338 + +0.1
1,097,338 + -1.0
1,097,339 - -0.1
1,097,339 - +0.0
1,097,460 - -1.8
1,097,610 - +0.4
1,097,610 - +2.4
1,097,610 - +0.5
1,097,613 - -0.1
1,097,613 - -0.6
1,097,704 + RR42_RS25650 0.49 +1.1
1,097,704 + RR42_RS25650 0.49 +1.3
1,097,798 + +0.2
1,097,799 - +0.1
1,097,799 - -0.3
1,097,901 + +0.3
1,097,908 + +0.1
1,097,908 + -0.2
1,097,989 - +0.4
1,098,000 - -0.0
1,098,007 + +0.1
1,098,059 - -0.1
1,098,095 - +0.1
1,098,508 + RR42_RS25655 0.17 +0.2
1,098,508 + RR42_RS25655 0.17 -0.0
1,098,508 + RR42_RS25655 0.17 -1.3
1,098,509 - RR42_RS25655 0.17 -0.1
1,098,509 - RR42_RS25655 0.17 +1.4
1,098,589 + RR42_RS25655 0.20 -0.6
1,098,589 + RR42_RS25655 0.20 +0.3
1,098,590 - RR42_RS25655 0.20 +0.6
1,098,590 - RR42_RS25655 0.20 +1.0
1,098,603 + RR42_RS25655 0.20 +0.2
1,098,604 - RR42_RS25655 0.20 -2.0
1,098,604 - RR42_RS25655 0.20 -0.3
1,098,772 + RR42_RS25655 0.25 -0.8
1,098,772 + RR42_RS25655 0.25 -0.8
1,098,773 - RR42_RS25655 0.25 -0.1
1,098,810 + RR42_RS25655 0.26 -0.6
1,098,811 - RR42_RS25655 0.26 +0.2
1,098,811 - RR42_RS25655 0.26 -1.0
1,099,030 + RR42_RS25655 0.32 +0.8
1,099,123 + RR42_RS25655 0.35 -0.4
1,099,366 + RR42_RS25655 0.42 +1.6
1,099,624 - RR42_RS25655 0.49 +0.6
1,099,825 + RR42_RS25655 0.55 -0.2
1,099,825 + RR42_RS25655 0.55 -0.4
1,099,826 - RR42_RS25655 0.55 -0.8
1,100,018 - RR42_RS25655 0.60 +0.2
1,100,018 - RR42_RS25655 0.60 -0.2
1,100,029 + RR42_RS25655 0.61 +0.0
1,100,029 + RR42_RS25655 0.61 +0.0
1,100,030 - RR42_RS25655 0.61 -0.5
1,100,030 - RR42_RS25655 0.61 +1.0
1,100,032 + RR42_RS25655 0.61 +0.3
1,100,033 - RR42_RS25655 0.61 -0.6
1,100,287 - RR42_RS25655 0.68 +1.1
1,100,372 - RR42_RS25655 0.70 -0.0
1,100,372 - RR42_RS25655 0.70 +0.2
1,100,372 - RR42_RS25655 0.70 -0.3
1,100,372 - RR42_RS25655 0.70 -0.6
1,100,384 + RR42_RS25655 0.71 +0.7
1,100,393 - RR42_RS25655 0.71 +2.6
1,100,395 + RR42_RS25655 0.71 -1.3
1,100,395 + RR42_RS25655 0.71 -0.4
1,100,536 + RR42_RS25655 0.75 -0.5
1,100,537 - RR42_RS25655 0.75 +0.1
1,100,894 - RR42_RS25655 0.85 -0.1
1,100,936 - RR42_RS25655 0.86 +1.2
1,100,938 - RR42_RS25655 0.86 -0.3
1,100,938 - RR42_RS25655 0.86 +0.2
1,100,943 + RR42_RS25655 0.87 -0.5
1,101,352 - +0.2
1,101,456 + +0.2
1,101,457 - +0.4
1,101,642 - RR42_RS25660 0.33 -0.3
1,101,663 + RR42_RS25660 0.36 +0.1
1,101,664 - RR42_RS25660 0.36 -0.6
1,101,673 - RR42_RS25660 0.38 -0.1
1,101,732 + RR42_RS25660 0.46 +0.6
1,101,994 - RR42_RS25660 0.85 +0.3
1,102,072 - -0.5
1,102,143 + +0.2
1,102,144 - +0.5
1,102,388 + -1.6
1,102,389 - -2.3
1,102,389 - +0.1

Or see this region's nucleotide sequence