Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21275 and RR42_RS21280 are separated by 51 nucleotides RR42_RS21280 and RR42_RS21285 are separated by 510 nucleotides
RR42_RS21275: RR42_RS21275 - carnitine dehydratase, at 98,655 to 99,806
_RS21275
RR42_RS21280: RR42_RS21280 - L-lactate permease, at 99,858 to 101,600
_RS21280
RR42_RS21285: RR42_RS21285 - L-lactate dehydrogenase, LutB subunit <small>(from data)</small>, at 102,111 to 103,541
_RS21285
Position (kb) 99
100
101
102 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 98.944 kb on + strand, within RR42_RS21275 at 98.944 kb on + strand, within RR42_RS21275 at 98.945 kb on - strand, within RR42_RS21275 at 98.945 kb on - strand, within RR42_RS21275 at 99.270 kb on + strand, within RR42_RS21275 at 99.270 kb on + strand, within RR42_RS21275 at 99.270 kb on + strand, within RR42_RS21275 at 99.270 kb on + strand, within RR42_RS21275 at 99.270 kb on + strand, within RR42_RS21275 at 99.271 kb on - strand, within RR42_RS21275 at 99.271 kb on - strand, within RR42_RS21275 at 99.366 kb on + strand, within RR42_RS21275 at 99.367 kb on - strand, within RR42_RS21275 at 99.582 kb on - strand, within RR42_RS21275 at 99.690 kb on + strand, within RR42_RS21275 at 99.738 kb on + strand at 99.739 kb on - strand at 99.745 kb on - strand at 99.886 kb on + strand at 99.886 kb on + strand at 99.886 kb on + strand at 99.886 kb on + strand at 99.886 kb on + strand at 99.886 kb on + strand at 99.887 kb on - strand at 99.887 kb on - strand at 100.246 kb on + strand, within RR42_RS21280 at 100.246 kb on + strand, within RR42_RS21280 at 100.273 kb on - strand, within RR42_RS21280 at 100.411 kb on - strand, within RR42_RS21280 at 100.438 kb on + strand, within RR42_RS21280 at 100.637 kb on - strand, within RR42_RS21280 at 100.637 kb on - strand, within RR42_RS21280 at 100.868 kb on + strand, within RR42_RS21280 at 100.885 kb on + strand, within RR42_RS21280 at 100.885 kb on + strand, within RR42_RS21280 at 100.886 kb on - strand, within RR42_RS21280 at 100.886 kb on - strand, within RR42_RS21280 at 100.886 kb on - strand, within RR42_RS21280 at 101.315 kb on - strand, within RR42_RS21280 at 101.329 kb on + strand, within RR42_RS21280 at 101.377 kb on + strand, within RR42_RS21280 at 101.428 kb on + strand at 101.568 kb on + strand at 101.569 kb on - strand at 101.569 kb on - strand at 101.578 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 101.701 kb on + strand at 102.070 kb on + strand at 102.071 kb on - strand at 102.112 kb on + strand at 102.167 kb on + strand at 102.534 kb on - strand, within RR42_RS21285 at 102.534 kb on - strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285 at 102.565 kb on + strand, within RR42_RS21285
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 98,944 + RR42_RS21275 0.25 +0.4 98,944 + RR42_RS21275 0.25 +1.3 98,945 - RR42_RS21275 0.25 +0.7 98,945 - RR42_RS21275 0.25 +0.1 99,270 + RR42_RS21275 0.53 -1.0 99,270 + RR42_RS21275 0.53 -0.6 99,270 + RR42_RS21275 0.53 -0.8 99,270 + RR42_RS21275 0.53 -0.0 99,270 + RR42_RS21275 0.53 -0.2 99,271 - RR42_RS21275 0.53 -2.2 99,271 - RR42_RS21275 0.53 +0.5 99,366 + RR42_RS21275 0.62 +0.6 99,367 - RR42_RS21275 0.62 +1.6 99,582 - RR42_RS21275 0.80 -0.1 99,690 + RR42_RS21275 0.90 +0.5 99,738 + -1.5 99,739 - -0.2 99,745 - -0.5 99,886 + -3.0 99,886 + -0.1 99,886 + -3.8 99,886 + -1.9 99,886 + -0.0 99,886 + +0.7 99,887 - +0.3 99,887 - -2.9 100,246 + RR42_RS21280 0.22 -2.5 100,246 + RR42_RS21280 0.22 -2.6 100,273 - RR42_RS21280 0.24 -2.2 100,411 - RR42_RS21280 0.32 -2.1 100,438 + RR42_RS21280 0.33 -0.5 100,637 - RR42_RS21280 0.45 -2.5 100,637 - RR42_RS21280 0.45 -2.5 100,868 + RR42_RS21280 0.58 -0.6 100,885 + RR42_RS21280 0.59 -3.4 100,885 + RR42_RS21280 0.59 -2.7 100,886 - RR42_RS21280 0.59 -1.9 100,886 - RR42_RS21280 0.59 -1.4 100,886 - RR42_RS21280 0.59 -1.9 101,315 - RR42_RS21280 0.84 -2.4 101,329 + RR42_RS21280 0.84 -0.5 101,377 + RR42_RS21280 0.87 -2.9 101,428 + -2.2 101,568 + -2.3 101,569 - -1.5 101,569 - -1.9 101,578 + -1.7 101,701 + -0.7 101,701 + -1.9 101,701 + -1.8 101,701 + -2.9 102,070 + -0.8 102,071 - -1.3 102,112 + -0.7 102,167 + -0.4 102,534 - RR42_RS21285 0.30 -2.0 102,534 - RR42_RS21285 0.30 -2.0 102,565 + RR42_RS21285 0.32 -1.5 102,565 + RR42_RS21285 0.32 -0.3 102,565 + RR42_RS21285 0.32 -1.7 102,565 + RR42_RS21285 0.32 -1.9
Or see this region's nucleotide sequence