Experiment: LB, 23C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS05390 and BPHYT_RS05395 are separated by 39 nucleotides BPHYT_RS05395 and BPHYT_RS05400 are separated by 344 nucleotides BPHYT_RS05400 and BPHYT_RS05405 are separated by 33 nucleotides BPHYT_RS05405 and BPHYT_RS05410 are separated by 144 nucleotides
BPHYT_RS05390: BPHYT_RS05390 - isochorismatase, at 1,239,752 to 1,240,399
_RS05390
BPHYT_RS05395: BPHYT_RS05395 - hypothetical protein, at 1,240,439 to 1,240,627
_RS05395
BPHYT_RS05400: BPHYT_RS05400 - hypothetical protein, at 1,240,972 to 1,241,418
_RS05400
BPHYT_RS05405: BPHYT_RS05405 - GCN5 family acetyltransferase, at 1,241,452 to 1,241,955
_RS05405
BPHYT_RS05410: BPHYT_RS05410 - beta-lactamase, at 1,242,100 to 1,243,104
_RS05410
Position (kb) 1240
1241
1242 Strain fitness (log2 ratio) -1
0
1
2 at 1240.065 kb on + strand, within BPHYT_RS05390 at 1240.249 kb on + strand, within BPHYT_RS05390 at 1240.250 kb on - strand, within BPHYT_RS05390 at 1240.367 kb on - strand at 1240.441 kb on - strand at 1240.517 kb on - strand, within BPHYT_RS05395 at 1241.499 kb on - strand at 1241.670 kb on - strand, within BPHYT_RS05405 at 1241.866 kb on + strand, within BPHYT_RS05405 at 1241.872 kb on - strand, within BPHYT_RS05405 at 1241.994 kb on + strand at 1241.995 kb on - strand at 1241.997 kb on - strand at 1242.067 kb on + strand at 1242.069 kb on + strand at 1242.161 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, 23C remove 1,240,065 + BPHYT_RS05390 0.48 +1.2 1,240,249 + BPHYT_RS05390 0.77 +0.2 1,240,250 - BPHYT_RS05390 0.77 +0.4 1,240,367 - -0.5 1,240,441 - -0.0 1,240,517 - BPHYT_RS05395 0.41 +0.9 1,241,499 - -0.3 1,241,670 - BPHYT_RS05405 0.43 -1.0 1,241,866 + BPHYT_RS05405 0.82 -0.7 1,241,872 - BPHYT_RS05405 0.83 -0.4 1,241,994 + -0.0 1,241,995 - +0.6 1,241,997 - +2.4 1,242,067 + -0.6 1,242,069 + +0.3 1,242,161 - -0.0
Or see this region's nucleotide sequence