Experiment: CDM1 without Tryptophan
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10895 and BBR_RS10900 are separated by 246 nucleotides BBR_RS10900 and BBR_RS10905 overlap by 1 nucleotides BBR_RS10905 and BBR_RS10910 are separated by 178 nucleotides
BBR_RS10895: BBR_RS10895 - type I pullulanase, at 183,482 to 188,608
_RS10895
BBR_RS10900: BBR_RS10900 - molecular chaperone DnaK, at 188,855 to 190,735
_RS10900
BBR_RS10905: BBR_RS10905 - nucleotide exchange factor GrpE, at 190,735 to 191,418
_RS10905
BBR_RS10910: BBR_RS10910 - molecular chaperone DnaJ, at 191,597 to 192,610
_RS10910
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 187.864 kb on + strand, within BBR_RS10895 at 187.867 kb on - strand, within BBR_RS10895 at 187.867 kb on - strand, within BBR_RS10895 at 187.872 kb on - strand, within BBR_RS10895 at 187.886 kb on + strand, within BBR_RS10895 at 187.898 kb on + strand, within BBR_RS10895 at 187.903 kb on + strand, within BBR_RS10895 at 187.906 kb on - strand, within BBR_RS10895 at 187.913 kb on + strand, within BBR_RS10895 at 187.914 kb on - strand, within BBR_RS10895 at 187.921 kb on + strand, within BBR_RS10895 at 187.950 kb on + strand, within BBR_RS10895 at 187.955 kb on + strand, within BBR_RS10895 at 187.955 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.960 kb on + strand, within BBR_RS10895 at 187.968 kb on - strand, within BBR_RS10895 at 187.977 kb on + strand, within BBR_RS10895 at 187.977 kb on + strand, within BBR_RS10895 at 187.982 kb on + strand, within BBR_RS10895 at 187.984 kb on - strand, within BBR_RS10895 at 187.987 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.992 kb on + strand, within BBR_RS10895 at 187.994 kb on + strand, within BBR_RS10895 at 187.994 kb on + strand, within BBR_RS10895 at 187.995 kb on + strand, within BBR_RS10895 at 187.995 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.000 kb on - strand, within BBR_RS10895 at 188.011 kb on + strand, within BBR_RS10895 at 188.013 kb on + strand, within BBR_RS10895 at 188.014 kb on + strand, within BBR_RS10895 at 188.019 kb on - strand, within BBR_RS10895 at 188.019 kb on - strand, within BBR_RS10895 at 188.021 kb on + strand, within BBR_RS10895 at 188.021 kb on + strand, within BBR_RS10895 at 188.024 kb on - strand, within BBR_RS10895 at 188.026 kb on + strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.029 kb on - strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.032 kb on + strand, within BBR_RS10895 at 188.040 kb on - strand, within BBR_RS10895 at 188.040 kb on - strand, within BBR_RS10895 at 188.048 kb on - strand, within BBR_RS10895 at 188.050 kb on - strand, within BBR_RS10895 at 188.050 kb on - strand, within BBR_RS10895 at 188.076 kb on + strand, within BBR_RS10895 at 188.079 kb on + strand, within BBR_RS10895 at 188.087 kb on - strand, within BBR_RS10895 at 188.094 kb on + strand, within BBR_RS10895 at 188.097 kb on - strand at 188.097 kb on - strand at 188.124 kb on - strand at 188.135 kb on + strand at 188.135 kb on + strand at 188.137 kb on + strand at 188.137 kb on + strand at 188.145 kb on - strand at 188.145 kb on - strand at 188.145 kb on - strand at 188.147 kb on + strand at 188.147 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.182 kb on + strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.190 kb on - strand at 188.191 kb on - strand at 188.192 kb on + strand at 188.213 kb on + strand at 188.232 kb on - strand at 188.238 kb on + strand at 188.238 kb on + strand at 188.244 kb on - strand at 188.246 kb on - strand at 188.246 kb on - strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.248 kb on + strand at 188.256 kb on - strand at 188.256 kb on - strand at 188.278 kb on - strand at 188.304 kb on + strand at 188.304 kb on + strand at 188.305 kb on - strand at 188.359 kb on + strand at 188.364 kb on + strand at 188.368 kb on - strand at 188.368 kb on - strand at 188.369 kb on + strand at 188.369 kb on + strand at 188.369 kb on + strand at 188.370 kb on - strand at 188.377 kb on - strand at 188.377 kb on - strand at 188.391 kb on + strand at 188.391 kb on + strand at 188.391 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.396 kb on + strand at 188.399 kb on - strand at 188.401 kb on + strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.404 kb on - strand at 188.413 kb on - strand at 188.413 kb on - strand at 188.417 kb on + strand at 188.425 kb on - strand at 188.451 kb on + strand at 188.451 kb on + strand at 188.459 kb on - strand at 188.461 kb on + strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.469 kb on - strand at 188.477 kb on + strand at 188.477 kb on + strand at 188.477 kb on + strand at 188.479 kb on + strand at 188.479 kb on + strand at 188.482 kb on - strand at 188.485 kb on + strand at 188.485 kb on - strand at 188.485 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.487 kb on - strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.490 kb on + strand at 188.494 kb on + strand at 188.495 kb on + strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.498 kb on - strand at 188.519 kb on + strand at 188.532 kb on - strand at 188.567 kb on - strand at 188.574 kb on - strand at 188.577 kb on - strand at 188.586 kb on + strand at 188.591 kb on - strand at 188.592 kb on - strand at 188.595 kb on + strand at 188.595 kb on + strand at 188.596 kb on - strand at 188.598 kb on + strand at 188.603 kb on + strand at 188.603 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.605 kb on + strand at 188.607 kb on + strand at 188.608 kb on + strand at 188.608 kb on - strand at 188.608 kb on - strand at 188.608 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.611 kb on - strand at 188.613 kb on - strand at 188.613 kb on - strand at 188.613 kb on - strand at 188.615 kb on - strand at 188.623 kb on - strand at 188.670 kb on + strand at 188.672 kb on - strand at 188.675 kb on + strand at 188.683 kb on - strand at 188.737 kb on - strand at 188.825 kb on + strand at 188.825 kb on + strand at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.516 kb on + strand, within BBR_RS10900 at 190.522 kb on + strand, within BBR_RS10900 at 190.530 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.540 kb on + strand, within BBR_RS10900 at 190.549 kb on + strand at 190.555 kb on + strand at 190.555 kb on + strand at 190.569 kb on + strand at 190.599 kb on + strand at 190.599 kb on + strand at 190.611 kb on + strand at 190.611 kb on + strand at 190.815 kb on + strand, within BBR_RS10905 at 190.815 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.998 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.108 kb on + strand, within BBR_RS10905 at 191.305 kb on + strand, within BBR_RS10905 at 191.390 kb on + strand at 191.395 kb on + strand at 191.422 kb on + strand at 191.425 kb on - strand at 191.425 kb on - strand at 191.433 kb on + strand at 191.472 kb on - strand at 191.478 kb on + strand at 191.478 kb on + strand at 191.484 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.499 kb on + strand at 191.499 kb on + strand at 191.507 kb on - strand at 191.519 kb on - strand at 191.527 kb on + strand at 191.535 kb on - strand at 191.543 kb on - strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.553 kb on - strand at 191.553 kb on - strand at 191.567 kb on + strand at 191.587 kb on + strand at 191.603 kb on + strand at 191.608 kb on - strand at 191.619 kb on + strand at 191.626 kb on + strand at 191.626 kb on + strand at 191.636 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.655 kb on - strand at 191.655 kb on - strand at 191.664 kb on - strand at 191.701 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.710 kb on + strand, within BBR_RS10910 at 191.712 kb on - strand, within BBR_RS10910 at 191.719 kb on + strand, within BBR_RS10910
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Tryptophan remove 187,864 + BBR_RS10895 0.85 -0.5 187,867 - BBR_RS10895 0.86 -0.5 187,867 - BBR_RS10895 0.86 -0.5 187,872 - BBR_RS10895 0.86 +0.2 187,886 + BBR_RS10895 0.86 +0.4 187,898 + BBR_RS10895 0.86 -0.2 187,903 + BBR_RS10895 0.86 -0.4 187,906 - BBR_RS10895 0.86 -0.1 187,913 + BBR_RS10895 0.86 -0.0 187,914 - BBR_RS10895 0.86 +0.0 187,921 + BBR_RS10895 0.87 +0.5 187,950 + BBR_RS10895 0.87 -0.2 187,955 + BBR_RS10895 0.87 +0.2 187,955 + BBR_RS10895 0.87 +0.6 187,960 + BBR_RS10895 0.87 +0.6 187,960 + BBR_RS10895 0.87 +0.2 187,960 + BBR_RS10895 0.87 +0.3 187,960 + BBR_RS10895 0.87 -0.1 187,960 + BBR_RS10895 0.87 +0.5 187,968 - BBR_RS10895 0.87 -0.0 187,977 + BBR_RS10895 0.88 -2.0 187,977 + BBR_RS10895 0.88 +0.6 187,982 + BBR_RS10895 0.88 +0.5 187,984 - BBR_RS10895 0.88 -0.1 187,987 + BBR_RS10895 0.88 +0.1 187,992 + BBR_RS10895 0.88 -0.0 187,992 + BBR_RS10895 0.88 +0.2 187,992 + BBR_RS10895 0.88 -0.1 187,994 + BBR_RS10895 0.88 +0.3 187,994 + BBR_RS10895 0.88 +0.7 187,995 + BBR_RS10895 0.88 +0.6 187,995 - BBR_RS10895 0.88 -2.2 188,000 - BBR_RS10895 0.88 -0.7 188,000 - BBR_RS10895 0.88 -0.4 188,000 - BBR_RS10895 0.88 -0.1 188,011 + BBR_RS10895 0.88 +0.7 188,013 + BBR_RS10895 0.88 +1.1 188,014 + BBR_RS10895 0.88 +0.4 188,019 - BBR_RS10895 0.88 +0.6 188,019 - BBR_RS10895 0.88 -1.2 188,021 + BBR_RS10895 0.89 -0.2 188,021 + BBR_RS10895 0.89 +0.3 188,024 - BBR_RS10895 0.89 +0.1 188,026 + BBR_RS10895 0.89 +0.2 188,029 - BBR_RS10895 0.89 -1.3 188,029 - BBR_RS10895 0.89 +0.2 188,029 - BBR_RS10895 0.89 -2.1 188,029 - BBR_RS10895 0.89 +0.1 188,032 + BBR_RS10895 0.89 +0.9 188,032 + BBR_RS10895 0.89 +0.5 188,032 + BBR_RS10895 0.89 +0.7 188,040 - BBR_RS10895 0.89 -0.0 188,040 - BBR_RS10895 0.89 -0.9 188,048 - BBR_RS10895 0.89 -0.5 188,050 - BBR_RS10895 0.89 +0.4 188,050 - BBR_RS10895 0.89 +0.2 188,076 + BBR_RS10895 0.90 -0.0 188,079 + BBR_RS10895 0.90 +1.8 188,087 - BBR_RS10895 0.90 -0.1 188,094 + BBR_RS10895 0.90 +0.7 188,097 - +0.4 188,097 - -1.7 188,124 - -0.3 188,135 + +1.6 188,135 + -0.4 188,137 + -4.2 188,137 + +0.3 188,145 - -0.5 188,145 - +0.1 188,145 - -1.4 188,147 + +0.6 188,147 + -0.5 188,182 + +0.0 188,182 + +0.9 188,182 + +0.1 188,182 + +0.5 188,182 + -0.9 188,182 + -1.9 188,190 - +0.5 188,190 - +0.0 188,190 - -2.1 188,190 - -0.9 188,191 - +0.6 188,192 + +1.3 188,213 + -0.9 188,232 - -0.2 188,238 + +0.1 188,238 + +0.5 188,244 - -0.5 188,246 - -0.2 188,246 - -1.5 188,248 + -0.4 188,248 + +0.1 188,248 + +0.3 188,248 + -0.0 188,248 + -0.1 188,248 + +0.3 188,256 - +0.5 188,256 - +0.3 188,278 - +0.3 188,304 + -0.8 188,304 + +0.4 188,305 - +0.7 188,359 + +0.1 188,364 + +0.9 188,368 - -0.3 188,368 - -1.0 188,369 + +0.3 188,369 + -0.4 188,369 + -0.3 188,370 - +0.2 188,377 - -0.9 188,377 - +0.2 188,391 + +0.3 188,391 + -0.4 188,391 + -0.5 188,396 + +1.0 188,396 + -0.3 188,396 + +0.0 188,396 + +1.4 188,396 + +0.6 188,399 - -0.8 188,401 + +0.8 188,404 - -0.5 188,404 - +0.1 188,404 - +0.8 188,404 - +0.7 188,404 - -0.5 188,404 - +0.3 188,413 - +0.5 188,413 - -0.1 188,417 + +0.1 188,425 - +1.0 188,451 + -0.0 188,451 + +0.7 188,459 - +0.4 188,461 + -0.7 188,469 - +0.7 188,469 - +0.4 188,469 - -0.1 188,469 - -0.9 188,469 - +0.5 188,477 + +0.5 188,477 + +0.7 188,477 + +0.3 188,479 + +1.1 188,479 + +0.5 188,482 - +0.2 188,485 + -0.1 188,485 - +0.5 188,485 - -0.9 188,487 - +0.3 188,487 - -0.6 188,487 - +0.1 188,487 - +0.2 188,487 - +0.1 188,487 - -0.3 188,490 + +0.1 188,490 + -0.6 188,490 + +1.7 188,490 + +0.6 188,490 + -0.5 188,490 + +0.2 188,490 + +0.1 188,490 + +0.7 188,494 + -0.2 188,495 + +0.0 188,498 - -0.8 188,498 - -1.1 188,498 - -0.2 188,498 - -0.9 188,498 - -0.2 188,519 + -0.1 188,532 - +0.6 188,567 - -0.6 188,574 - +0.4 188,577 - -1.5 188,586 + -1.5 188,591 - +0.0 188,592 - +0.2 188,595 + +0.7 188,595 + -0.5 188,596 - +0.2 188,598 + -0.3 188,603 + +0.3 188,603 + +0.5 188,605 + -1.4 188,605 + +0.4 188,605 + +0.1 188,605 + +0.2 188,607 + -0.1 188,608 + +0.1 188,608 - -0.4 188,608 - -2.9 188,608 - -0.2 188,611 - +0.9 188,611 - +0.8 188,611 - -0.0 188,611 - +0.9 188,613 - +0.8 188,613 - -0.4 188,613 - -0.1 188,615 - -0.1 188,623 - -0.1 188,670 + +0.3 188,672 - -0.1 188,675 + -0.4 188,683 - -0.1 188,737 - +0.7 188,825 + -4.6 188,825 + -3.5 190,516 + BBR_RS10900 0.88 -1.4 190,516 + BBR_RS10900 0.88 -3.9 190,516 + BBR_RS10900 0.88 -3.3 190,522 + BBR_RS10900 0.89 -2.3 190,530 + BBR_RS10900 0.89 -1.4 190,540 + BBR_RS10900 0.90 -2.5 190,540 + BBR_RS10900 0.90 -2.4 190,549 + -2.2 190,555 + -3.3 190,555 + -2.5 190,569 + -2.8 190,599 + -0.1 190,599 + -4.0 190,611 + -1.6 190,611 + -2.8 190,815 + BBR_RS10905 0.12 -0.1 190,815 + BBR_RS10905 0.12 -0.3 190,845 + BBR_RS10905 0.16 -3.8 190,845 + BBR_RS10905 0.16 +0.7 190,845 + BBR_RS10905 0.16 -3.3 190,860 + BBR_RS10905 0.18 -2.3 190,860 + BBR_RS10905 0.18 -1.5 190,998 + BBR_RS10905 0.38 -4.7 191,022 + BBR_RS10905 0.42 -4.2 191,022 + BBR_RS10905 0.42 -4.2 191,022 + BBR_RS10905 0.42 -4.8 191,022 + BBR_RS10905 0.42 -3.1 191,108 + BBR_RS10905 0.55 -2.1 191,305 + BBR_RS10905 0.83 +0.5 191,390 + +1.6 191,395 + -0.5 191,422 + +0.9 191,425 - -3.3 191,425 - -2.8 191,433 + +0.2 191,472 - -4.1 191,478 + -0.1 191,478 + +0.7 191,484 + +1.2 191,488 + +0.3 191,488 + +1.1 191,488 + +0.5 191,488 + +0.9 191,488 + +0.7 191,496 - -1.8 191,496 - -2.9 191,496 - -2.4 191,499 + +0.3 191,499 + -2.1 191,507 - -1.1 191,519 - -4.6 191,527 + -0.4 191,535 - -4.0 191,543 - -2.1 191,545 + -0.9 191,545 + +0.9 191,545 + +0.6 191,545 + +0.7 191,545 + -0.2 191,553 - -4.7 191,553 - -1.3 191,567 + -0.3 191,587 + +0.3 191,603 + -3.1 191,608 - -3.1 191,619 + -1.2 191,626 + -3.2 191,626 + -3.8 191,636 + -0.5 191,647 + -5.1 191,647 + -4.1 191,647 + -2.9 191,655 - -2.0 191,655 - -2.1 191,664 - -3.4 191,701 + BBR_RS10910 0.10 -4.2 191,704 + BBR_RS10910 0.11 -2.2 191,704 + BBR_RS10910 0.11 -3.0 191,704 + BBR_RS10910 0.11 -2.6 191,704 + BBR_RS10910 0.11 -3.8 191,704 + BBR_RS10910 0.11 -1.3 191,704 + BBR_RS10910 0.11 -3.1 191,709 + BBR_RS10910 0.11 -3.4 191,709 + BBR_RS10910 0.11 -4.1 191,709 + BBR_RS10910 0.11 -3.2 191,710 + BBR_RS10910 0.11 -2.1 191,712 - BBR_RS10910 0.11 -2.1 191,719 + BBR_RS10910 0.12 -2.0
Or see this region's nucleotide sequence