Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21121 and SM_b21122 are separated by 27 nucleotides SM_b21122 and SM_b21123 are separated by 14 nucleotides SM_b21123 and SM_b21124 are separated by 16 nucleotides
SM_b21121: SM_b21121 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) <small>(from data)</small>, at 759,569 to 760,732
_b21121
SM_b21122: SM_b21122 - 3-methylcrotonyl-CoA carboxylase, beta subunit (EC 6.4.1.4) <small>(from data)</small>, at 760,760 to 762,367
_b21122
SM_b21123: SM_b21123 - translation initiation inhibitor, at 762,382 to 762,765
_b21123
SM_b21124: SM_b21124 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) <small>(from data)</small>, at 762,782 to 764,770
_b21124
Position (kb) 760
761
762
763 Strain fitness (log2 ratio) -2
-1
0
1 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.843 kb on - strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.912 kb on - strand, within SM_b21121 at 760.025 kb on + strand, within SM_b21121 at 760.026 kb on - strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.089 kb on - strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.448 kb on + strand at 760.448 kb on + strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.481 kb on + strand, within SM_b21121 at 760.540 kb on + strand, within SM_b21121 at 760.588 kb on - strand, within SM_b21121 at 760.588 kb on - strand, within SM_b21121 at 760.611 kb on - strand, within SM_b21121 at 760.658 kb on + strand at 760.740 kb on - strand at 760.743 kb on + strand at 760.768 kb on - strand at 760.942 kb on + strand, within SM_b21122 at 760.994 kb on + strand, within SM_b21122 at 761.003 kb on + strand, within SM_b21122 at 761.003 kb on + strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.004 kb on - strand, within SM_b21122 at 761.108 kb on + strand, within SM_b21122 at 761.108 kb on + strand, within SM_b21122 at 761.109 kb on - strand, within SM_b21122 at 761.112 kb on - strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.180 kb on + strand, within SM_b21122 at 761.181 kb on - strand, within SM_b21122 at 761.181 kb on - strand, within SM_b21122 at 761.262 kb on - strand, within SM_b21122 at 761.262 kb on - strand, within SM_b21122 at 761.263 kb on + strand, within SM_b21122 at 761.339 kb on + strand, within SM_b21122 at 761.339 kb on + strand, within SM_b21122 at 761.340 kb on - strand, within SM_b21122 at 761.340 kb on - strand, within SM_b21122 at 761.390 kb on - strand, within SM_b21122 at 761.609 kb on + strand, within SM_b21122 at 761.660 kb on + strand, within SM_b21122 at 761.661 kb on - strand, within SM_b21122 at 761.912 kb on + strand, within SM_b21122 at 762.023 kb on + strand, within SM_b21122 at 762.210 kb on + strand at 762.211 kb on - strand at 762.236 kb on + strand at 762.237 kb on - strand at 762.237 kb on - strand at 762.497 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.670 kb on + strand, within SM_b21123 at 762.671 kb on - strand, within SM_b21123 at 762.763 kb on + strand at 762.763 kb on + strand at 762.764 kb on - strand at 762.782 kb on - strand at 762.782 kb on - strand at 762.959 kb on + strand at 762.977 kb on - strand at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.083 kb on + strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124 at 763.084 kb on - strand, within SM_b21124
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 759,811 - SM_b21121 0.21 -0.1 759,811 - SM_b21121 0.21 +0.0 759,811 - SM_b21121 0.21 -1.2 759,843 - SM_b21121 0.24 +0.0 759,911 + SM_b21121 0.29 +0.2 759,911 + SM_b21121 0.29 -0.2 759,911 + SM_b21121 0.29 +0.4 759,912 - SM_b21121 0.29 +0.2 760,025 + SM_b21121 0.39 -0.3 760,026 - SM_b21121 0.39 -0.1 760,088 + SM_b21121 0.45 -0.2 760,088 + SM_b21121 0.45 -0.3 760,089 - SM_b21121 0.45 +0.0 760,288 + SM_b21121 0.62 -0.5 760,288 + SM_b21121 0.62 -1.0 760,288 + SM_b21121 0.62 -0.3 760,288 + SM_b21121 0.62 +0.1 760,288 + SM_b21121 0.62 -0.7 760,289 - SM_b21121 0.62 +0.4 760,289 - SM_b21121 0.62 -2.0 760,374 - SM_b21121 0.69 -1.0 760,374 - SM_b21121 0.69 -0.0 760,374 - SM_b21121 0.69 -0.5 760,448 + +0.6 760,448 + SM_b21121 0.76 -0.0 760,449 - SM_b21121 0.76 +0.0 760,449 - SM_b21121 0.76 +0.1 760,449 - SM_b21121 0.76 +0.3 760,449 - SM_b21121 0.76 +0.1 760,449 - SM_b21121 0.76 +0.5 760,449 - SM_b21121 0.76 -0.3 760,475 + SM_b21121 0.78 -0.2 760,475 + SM_b21121 0.78 -0.4 760,476 - SM_b21121 0.78 -0.4 760,476 - SM_b21121 0.78 +0.3 760,476 - SM_b21121 0.78 -1.7 760,476 - SM_b21121 0.78 +0.1 760,481 + SM_b21121 0.78 -0.3 760,540 + SM_b21121 0.83 +0.2 760,588 - SM_b21121 0.88 +0.1 760,588 - SM_b21121 0.88 +0.9 760,611 - SM_b21121 0.90 +0.6 760,658 + +1.0 760,740 - -0.3 760,743 + -0.4 760,768 - -0.2 760,942 + SM_b21122 0.11 +0.5 760,994 + SM_b21122 0.15 -0.6 761,003 + SM_b21122 0.15 -0.7 761,003 + SM_b21122 0.15 +0.4 761,004 - SM_b21122 0.15 +0.4 761,004 - SM_b21122 0.15 -0.5 761,004 - SM_b21122 0.15 -0.3 761,004 - SM_b21122 0.15 -0.3 761,108 + SM_b21122 0.22 -0.1 761,108 + SM_b21122 0.22 -0.9 761,109 - SM_b21122 0.22 +0.2 761,112 - SM_b21122 0.22 +0.3 761,180 + SM_b21122 0.26 -0.4 761,180 + SM_b21122 0.26 +0.5 761,180 + SM_b21122 0.26 -0.9 761,181 - SM_b21122 0.26 +0.0 761,181 - SM_b21122 0.26 -0.2 761,262 - SM_b21122 0.31 -1.0 761,262 - SM_b21122 0.31 +0.2 761,263 + SM_b21122 0.31 -1.2 761,339 + SM_b21122 0.36 -0.7 761,339 + SM_b21122 0.36 +0.2 761,340 - SM_b21122 0.36 -0.4 761,340 - SM_b21122 0.36 -0.5 761,390 - SM_b21122 0.39 -0.3 761,609 + SM_b21122 0.53 -0.3 761,660 + SM_b21122 0.56 -0.0 761,661 - SM_b21122 0.56 -0.1 761,912 + SM_b21122 0.72 -1.0 762,023 + SM_b21122 0.79 -0.2 762,210 + +0.1 762,211 - -0.4 762,236 + +1.3 762,237 - -0.8 762,237 - +0.6 762,497 + SM_b21123 0.30 +0.3 762,670 + SM_b21123 0.75 -0.2 762,670 + SM_b21123 0.75 -0.1 762,671 - SM_b21123 0.75 -0.4 762,763 + -0.5 762,763 + -0.0 762,764 - -0.7 762,782 - -0.5 762,782 - -0.1 762,959 + -1.3 762,977 - +1.6 763,083 + SM_b21124 0.15 -0.7 763,083 + SM_b21124 0.15 -0.8 763,083 + SM_b21124 0.15 -0.4 763,084 - SM_b21124 0.15 -0.1 763,084 - SM_b21124 0.15 -1.7
Or see this region's nucleotide sequence