Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20018 and SM_b20019 are separated by 91 nucleotides SM_b20019 and SM_b20020 are separated by 2 nucleotides
SM_b20018: SM_b20018 - sugar ABC transporter substrate-binding protein, at 25,092 to 26,114
_b20018
SM_b20019: SM_b20019 - dihydrolipoamide succinyltransferase, at 26,206 to 27,342
_b20019
SM_b20020: SM_b20020 - pyruvate dehydrogenase E1 component, subunits alpha and beta, at 27,345 to 29,423
_b20020
Position (kb)
26
27
28 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 25.220 kb on - strand, within SM_b20018 at 25.489 kb on + strand, within SM_b20018 at 25.653 kb on + strand, within SM_b20018 at 25.654 kb on - strand, within SM_b20018 at 25.654 kb on - strand, within SM_b20018 at 25.654 kb on - strand, within SM_b20018 at 25.654 kb on - strand, within SM_b20018 at 25.732 kb on + strand, within SM_b20018 at 25.732 kb on + strand, within SM_b20018 at 25.732 kb on + strand, within SM_b20018 at 25.732 kb on + strand, within SM_b20018 at 25.733 kb on - strand, within SM_b20018 at 25.880 kb on + strand, within SM_b20018 at 26.036 kb on + strand at 26.114 kb on + strand at 26.208 kb on - strand at 26.208 kb on - strand at 26.303 kb on + strand at 26.304 kb on - strand at 26.304 kb on - strand at 26.304 kb on - strand at 26.615 kb on - strand, within SM_b20019 at 26.615 kb on - strand, within SM_b20019 at 26.615 kb on - strand, within SM_b20019 at 26.622 kb on + strand, within SM_b20019 at 26.623 kb on - strand, within SM_b20019 at 26.718 kb on - strand, within SM_b20019 at 26.726 kb on + strand, within SM_b20019 at 26.850 kb on + strand, within SM_b20019 at 26.850 kb on + strand, within SM_b20019 at 26.851 kb on - strand, within SM_b20019 at 26.851 kb on - strand, within SM_b20019 at 26.851 kb on - strand, within SM_b20019 at 26.888 kb on - strand, within SM_b20019 at 26.888 kb on - strand, within SM_b20019 at 27.041 kb on + strand, within SM_b20019 at 27.042 kb on - strand, within SM_b20019 at 27.042 kb on - strand, within SM_b20019 at 27.378 kb on + strand at 28.057 kb on + strand, within SM_b20020 at 28.058 kb on - strand, within SM_b20020 at 28.058 kb on - strand, within SM_b20020
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 25,220 - SM_b20018 0.13 +0.3 25,489 + SM_b20018 0.39 -0.4 25,653 + SM_b20018 0.55 +0.2 25,654 - SM_b20018 0.55 -0.7 25,654 - SM_b20018 0.55 +1.8 25,654 - SM_b20018 0.55 +0.1 25,654 - SM_b20018 0.55 -1.1 25,732 + SM_b20018 0.63 +0.6 25,732 + SM_b20018 0.63 -0.3 25,732 + SM_b20018 0.63 -0.1 25,732 + SM_b20018 0.63 -0.6 25,733 - SM_b20018 0.63 -0.5 25,880 + SM_b20018 0.77 -0.3 26,036 + -0.5 26,114 + -2.2 26,208 - +0.6 26,208 - +0.0 26,303 + +1.2 26,304 - -0.1 26,304 - -0.3 26,304 - -0.8 26,615 - SM_b20019 0.36 -0.3 26,615 - SM_b20019 0.36 -0.7 26,615 - SM_b20019 0.36 -0.7 26,622 + SM_b20019 0.37 -1.0 26,623 - SM_b20019 0.37 -0.1 26,718 - SM_b20019 0.45 -0.2 26,726 + SM_b20019 0.46 -0.4 26,850 + SM_b20019 0.57 -0.0 26,850 + SM_b20019 0.57 -1.4 26,851 - SM_b20019 0.57 -0.0 26,851 - SM_b20019 0.57 -0.7 26,851 - SM_b20019 0.57 -0.0 26,888 - SM_b20019 0.60 -0.5 26,888 - SM_b20019 0.60 -0.2 27,041 + SM_b20019 0.73 -0.0 27,042 - SM_b20019 0.74 -1.4 27,042 - SM_b20019 0.74 +0.2 27,378 + -0.6 28,057 + SM_b20020 0.34 +0.7 28,058 - SM_b20020 0.34 +0.4 28,058 - SM_b20020 0.34 +0.3
Or see this region's nucleotide sequence