Experiment: CDM1 without Tyrosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16680 and BBR_RS16685 are separated by 58 nucleotides BBR_RS16685 and BBR_RS16690 are separated by 132 nucleotides BBR_RS16690 and BBR_RS16695 are separated by 389 nucleotides
BBR_RS16680: BBR_RS16680 - chorismate mutase, at 1,513,388 to 1,514,368
_RS16680
BBR_RS16685: BBR_RS16685 - alcohol dehydrogenase, at 1,514,427 to 1,514,867
_RS16685
BBR_RS16690: BBR_RS16690 - translational GTPase TypA, at 1,515,000 to 1,516,931
_RS16690
BBR_RS16695: BBR_RS16695 - PadR family transcriptional regulator, at 1,517,321 to 1,517,926
_RS16695
Position (kb)
1514
1515
1516
1517 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.022 kb on - strand, within BBR_RS16680 at 1514.072 kb on + strand, within BBR_RS16680 at 1514.117 kb on + strand, within BBR_RS16680 at 1514.125 kb on - strand, within BBR_RS16680 at 1514.125 kb on - strand, within BBR_RS16680 at 1514.152 kb on - strand, within BBR_RS16680 at 1514.152 kb on - strand, within BBR_RS16680 at 1514.175 kb on - strand, within BBR_RS16680 at 1514.176 kb on - strand, within BBR_RS16680 at 1514.213 kb on - strand, within BBR_RS16680 at 1514.213 kb on - strand, within BBR_RS16680 at 1514.273 kb on - strand at 1514.371 kb on + strand at 1514.439 kb on + strand at 1514.452 kb on - strand at 1514.454 kb on + strand at 1514.480 kb on + strand, within BBR_RS16685 at 1514.485 kb on + strand, within BBR_RS16685 at 1514.825 kb on - strand at 1514.861 kb on - strand at 1514.889 kb on - strand at 1514.895 kb on - strand at 1514.919 kb on + strand at 1514.923 kb on - strand at 1514.927 kb on - strand at 1514.972 kb on - strand at 1515.009 kb on - strand at 1516.981 kb on - strand at 1517.058 kb on - strand at 1517.058 kb on - strand at 1517.058 kb on - strand at 1517.168 kb on + strand at 1517.219 kb on + strand at 1517.220 kb on - strand at 1517.358 kb on - strand at 1517.373 kb on - strand at 1517.378 kb on - strand at 1517.378 kb on - strand at 1517.378 kb on - strand at 1517.388 kb on - strand, within BBR_RS16695 at 1517.416 kb on - strand, within BBR_RS16695 at 1517.448 kb on - strand, within BBR_RS16695 at 1517.450 kb on - strand, within BBR_RS16695 at 1517.455 kb on - strand, within BBR_RS16695 at 1517.485 kb on - strand, within BBR_RS16695 at 1517.515 kb on + strand, within BBR_RS16695 at 1517.537 kb on + strand, within BBR_RS16695 at 1517.537 kb on + strand, within BBR_RS16695 at 1517.600 kb on - strand, within BBR_RS16695 at 1517.611 kb on + strand, within BBR_RS16695 at 1517.614 kb on - strand, within BBR_RS16695 at 1517.619 kb on - strand, within BBR_RS16695 at 1517.664 kb on + strand, within BBR_RS16695 at 1517.672 kb on - strand, within BBR_RS16695 at 1517.690 kb on + strand, within BBR_RS16695 at 1517.725 kb on - strand, within BBR_RS16695 at 1517.834 kb on + strand, within BBR_RS16695 at 1517.842 kb on - strand, within BBR_RS16695 at 1517.861 kb on + strand, within BBR_RS16695 at 1517.861 kb on + strand, within BBR_RS16695 at 1517.884 kb on + strand at 1517.906 kb on - strand at 1517.918 kb on + strand at 1517.926 kb on - strand at 1517.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Tyrosine remove 1,514,022 - BBR_RS16680 0.65 +0.8 1,514,022 - BBR_RS16680 0.65 +0.1 1,514,022 - BBR_RS16680 0.65 +0.0 1,514,072 + BBR_RS16680 0.70 -1.3 1,514,117 + BBR_RS16680 0.74 -2.3 1,514,125 - BBR_RS16680 0.75 -0.2 1,514,125 - BBR_RS16680 0.75 -0.2 1,514,152 - BBR_RS16680 0.78 -0.3 1,514,152 - BBR_RS16680 0.78 -0.8 1,514,175 - BBR_RS16680 0.80 -0.1 1,514,176 - BBR_RS16680 0.80 -0.1 1,514,213 - BBR_RS16680 0.84 +0.1 1,514,213 - BBR_RS16680 0.84 -0.2 1,514,273 - -0.5 1,514,371 + -0.2 1,514,439 + -1.3 1,514,452 - -0.2 1,514,454 + +1.1 1,514,480 + BBR_RS16685 0.12 -1.5 1,514,485 + BBR_RS16685 0.13 -0.4 1,514,825 - -0.7 1,514,861 - +1.9 1,514,889 - -0.1 1,514,895 - +0.3 1,514,919 + +0.1 1,514,923 - +0.7 1,514,927 - +0.4 1,514,972 - +1.9 1,515,009 - +0.7 1,516,981 - +0.3 1,517,058 - +0.2 1,517,058 - +0.0 1,517,058 - +0.5 1,517,168 + +0.1 1,517,219 + -1.3 1,517,220 - -0.4 1,517,358 - -0.5 1,517,373 - -0.3 1,517,378 - +0.1 1,517,378 - -0.7 1,517,378 - -1.4 1,517,388 - BBR_RS16695 0.11 -0.8 1,517,416 - BBR_RS16695 0.16 -1.3 1,517,448 - BBR_RS16695 0.21 -1.8 1,517,450 - BBR_RS16695 0.21 -0.6 1,517,455 - BBR_RS16695 0.22 -2.0 1,517,485 - BBR_RS16695 0.27 -0.3 1,517,515 + BBR_RS16695 0.32 +0.8 1,517,537 + BBR_RS16695 0.36 -1.8 1,517,537 + BBR_RS16695 0.36 -0.5 1,517,600 - BBR_RS16695 0.46 -1.2 1,517,611 + BBR_RS16695 0.48 -0.7 1,517,614 - BBR_RS16695 0.48 -1.8 1,517,619 - BBR_RS16695 0.49 -2.2 1,517,664 + BBR_RS16695 0.57 +0.4 1,517,672 - BBR_RS16695 0.58 +0.6 1,517,690 + BBR_RS16695 0.61 +0.9 1,517,725 - BBR_RS16695 0.67 -1.5 1,517,834 + BBR_RS16695 0.85 -1.3 1,517,842 - BBR_RS16695 0.86 +1.2 1,517,861 + BBR_RS16695 0.89 -0.2 1,517,861 + BBR_RS16695 0.89 -2.2 1,517,884 + -1.2 1,517,906 - +1.0 1,517,918 + -0.4 1,517,926 - -1.2 1,517,926 - +1.4
Or see this region's nucleotide sequence