Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20268 and SM_b20269 overlap by 4 nucleotides SM_b20269 and SM_b20270 are separated by 50 nucleotides
SM_b20268: SM_b20268 - hydroxyproline-2-epimerase, at 272,694 to 273,695
_b20268
SM_b20269: SM_b20269 - hypothetical protein, at 273,692 to 275,383
_b20269
SM_b20270: SM_b20270 - proline racemase, at 275,434 to 276,462
_b20270
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 272.936 kb on - strand, within SM_b20268 at 273.075 kb on + strand, within SM_b20268 at 273.075 kb on + strand, within SM_b20268 at 273.076 kb on - strand, within SM_b20268 at 273.076 kb on - strand, within SM_b20268 at 273.196 kb on - strand, within SM_b20268 at 273.257 kb on + strand, within SM_b20268 at 273.750 kb on + strand at 274.293 kb on - strand, within SM_b20269 at 274.293 kb on - strand, within SM_b20269 at 274.293 kb on - strand, within SM_b20269 at 274.293 kb on - strand, within SM_b20269 at 274.293 kb on - strand, within SM_b20269 at 274.457 kb on + strand, within SM_b20269 at 274.457 kb on + strand, within SM_b20269 at 274.696 kb on - strand, within SM_b20269 at 274.696 kb on - strand, within SM_b20269 at 275.007 kb on + strand, within SM_b20269 at 275.233 kb on + strand at 275.264 kb on + strand at 275.264 kb on + strand at 275.264 kb on + strand at 275.265 kb on - strand at 275.265 kb on - strand at 275.265 kb on - strand at 275.302 kb on - strand at 275.302 kb on - strand at 275.689 kb on - strand, within SM_b20270 at 275.847 kb on + strand, within SM_b20270 at 275.848 kb on - strand, within SM_b20270 at 275.848 kb on - strand, within SM_b20270 at 276.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 272,936 - SM_b20268 0.24 +0.7 273,075 + SM_b20268 0.38 -0.2 273,075 + SM_b20268 0.38 -1.6 273,076 - SM_b20268 0.38 +0.8 273,076 - SM_b20268 0.38 -0.0 273,196 - SM_b20268 0.50 -0.2 273,257 + SM_b20268 0.56 -0.3 273,750 + -0.2 274,293 - SM_b20269 0.36 +0.6 274,293 - SM_b20269 0.36 -0.0 274,293 - SM_b20269 0.36 -0.1 274,293 - SM_b20269 0.36 +0.3 274,293 - SM_b20269 0.36 +0.1 274,457 + SM_b20269 0.45 +1.6 274,457 + SM_b20269 0.45 -2.2 274,696 - SM_b20269 0.59 +0.2 274,696 - SM_b20269 0.59 +0.4 275,007 + SM_b20269 0.78 +0.0 275,233 + +3.2 275,264 + +0.2 275,264 + +0.9 275,264 + +0.2 275,265 - +0.1 275,265 - -0.2 275,265 - -0.9 275,302 - -0.5 275,302 - +0.0 275,689 - SM_b20270 0.25 +0.8 275,847 + SM_b20270 0.40 -1.2 275,848 - SM_b20270 0.40 -1.2 275,848 - SM_b20270 0.40 +0.8 276,378 + -0.4
Or see this region's nucleotide sequence