Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30115 and BPHYT_RS30120 are separated by 169 nucleotides BPHYT_RS30120 and BPHYT_RS30125 are separated by 29 nucleotides BPHYT_RS30125 and BPHYT_RS30130 are separated by 94 nucleotides BPHYT_RS30130 and BPHYT_RS30135 are separated by 230 nucleotides
BPHYT_RS30115: BPHYT_RS30115 - hypothetical protein, at 2,310,137 to 2,310,559
_RS30115
BPHYT_RS30120: BPHYT_RS30120 - heavy metal transporter, at 2,310,729 to 2,310,926
_RS30120
BPHYT_RS30125: BPHYT_RS30125 - hypothetical protein, at 2,310,956 to 2,311,369
_RS30125
BPHYT_RS30130: BPHYT_RS30130 - AraC family transcriptional regulator, at 2,311,464 to 2,312,450
_RS30130
BPHYT_RS30135: BPHYT_RS30135 - SAM-dependent methyltransferase, at 2,312,681 to 2,313,514
_RS30135
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-2
-1
0
1 at 2310.563 kb on + strand at 2310.572 kb on + strand at 2310.573 kb on - strand at 2310.749 kb on + strand, within BPHYT_RS30120 at 2310.852 kb on - strand, within BPHYT_RS30120 at 2311.656 kb on - strand, within BPHYT_RS30130 at 2311.845 kb on - strand, within BPHYT_RS30130 at 2312.093 kb on - strand, within BPHYT_RS30130 at 2312.765 kb on + strand, within BPHYT_RS30135 at 2313.109 kb on - strand, within BPHYT_RS30135 at 2313.356 kb on + strand, within BPHYT_RS30135
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 2,310,563 + -1.2 2,310,572 + -0.1 2,310,573 - +0.2 2,310,749 + BPHYT_RS30120 0.10 -0.0 2,310,852 - BPHYT_RS30120 0.62 +0.3 2,311,656 - BPHYT_RS30130 0.19 -0.9 2,311,845 - BPHYT_RS30130 0.39 -2.5 2,312,093 - BPHYT_RS30130 0.64 -0.1 2,312,765 + BPHYT_RS30135 0.10 +0.2 2,313,109 - BPHYT_RS30135 0.51 +0.5 2,313,356 + BPHYT_RS30135 0.81 -0.5
Or see this region's nucleotide sequence