Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00094 and SMc00127 are separated by 1 nucleotides SMc00127 and SMc00095 are separated by 166 nucleotides SMc00095 and SMc00032 are separated by 143 nucleotides
SMc00094: SMc00094 - betaine aldehyde dehydrogenase, at 1,041,153 to 1,042,616
SMc00094
SMc00127: SMc00127 - choline sulfatase, at 1,042,618 to 1,044,156
SMc00127
SMc00095: SMc00095 - transcriptional regulator BetI, at 1,044,323 to 1,044,934
SMc00095
SMc00032: SMc00032 - hypothetical protein, at 1,045,078 to 1,045,626
SMc00032
Position (kb)
1042
1043
1044
1045 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1042.058 kb on + strand, within SMc00094 at 1042.059 kb on - strand, within SMc00094 at 1042.156 kb on + strand, within SMc00094 at 1042.157 kb on - strand, within SMc00094 at 1042.205 kb on + strand, within SMc00094 at 1042.206 kb on - strand, within SMc00094 at 1042.207 kb on + strand, within SMc00094 at 1042.207 kb on + strand, within SMc00094 at 1042.232 kb on + strand, within SMc00094 at 1042.234 kb on + strand, within SMc00094 at 1042.234 kb on + strand, within SMc00094 at 1042.234 kb on + strand, within SMc00094 at 1042.235 kb on - strand, within SMc00094 at 1042.235 kb on - strand, within SMc00094 at 1042.535 kb on - strand at 1042.535 kb on - strand at 1042.573 kb on + strand at 1042.574 kb on - strand at 1042.574 kb on - strand at 1042.574 kb on - strand at 1042.683 kb on - strand at 1042.724 kb on + strand at 1042.724 kb on + strand at 1042.725 kb on - strand at 1042.727 kb on + strand at 1042.727 kb on + strand at 1042.727 kb on + strand at 1042.751 kb on + strand at 1042.752 kb on - strand at 1042.890 kb on + strand, within SMc00127 at 1042.891 kb on - strand, within SMc00127 at 1042.946 kb on + strand, within SMc00127 at 1042.947 kb on - strand, within SMc00127 at 1042.952 kb on + strand, within SMc00127 at 1042.953 kb on - strand, within SMc00127 at 1042.953 kb on - strand, within SMc00127 at 1042.991 kb on + strand, within SMc00127 at 1042.991 kb on + strand, within SMc00127 at 1042.991 kb on + strand, within SMc00127 at 1042.991 kb on + strand, within SMc00127 at 1042.992 kb on - strand, within SMc00127 at 1042.992 kb on - strand, within SMc00127 at 1042.992 kb on - strand, within SMc00127 at 1043.009 kb on + strand, within SMc00127 at 1043.010 kb on - strand, within SMc00127 at 1043.361 kb on + strand, within SMc00127 at 1043.362 kb on - strand, within SMc00127 at 1043.517 kb on - strand, within SMc00127 at 1043.546 kb on + strand, within SMc00127 at 1043.547 kb on - strand, within SMc00127 at 1043.666 kb on + strand, within SMc00127 at 1043.666 kb on + strand, within SMc00127 at 1043.667 kb on - strand, within SMc00127 at 1043.667 kb on - strand, within SMc00127 at 1043.789 kb on + strand, within SMc00127 at 1043.790 kb on - strand, within SMc00127 at 1043.870 kb on + strand, within SMc00127 at 1043.870 kb on + strand, within SMc00127 at 1043.871 kb on - strand, within SMc00127 at 1043.900 kb on + strand, within SMc00127 at 1043.900 kb on + strand, within SMc00127 at 1043.900 kb on + strand, within SMc00127 at 1043.900 kb on + strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.901 kb on - strand, within SMc00127 at 1043.921 kb on + strand, within SMc00127 at 1044.014 kb on + strand at 1044.014 kb on + strand at 1044.015 kb on - strand at 1044.144 kb on + strand at 1044.312 kb on + strand at 1044.313 kb on - strand at 1044.324 kb on + strand at 1044.381 kb on + strand at 1044.447 kb on + strand, within SMc00095 at 1044.447 kb on + strand, within SMc00095 at 1044.448 kb on - strand, within SMc00095 at 1044.448 kb on - strand, within SMc00095 at 1044.556 kb on - strand, within SMc00095 at 1044.556 kb on - strand, within SMc00095 at 1044.603 kb on + strand, within SMc00095 at 1044.604 kb on - strand, within SMc00095 at 1044.604 kb on - strand, within SMc00095 at 1044.856 kb on + strand, within SMc00095 at 1044.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 1,042,058 + SMc00094 0.62 -0.6 1,042,059 - SMc00094 0.62 -1.4 1,042,156 + SMc00094 0.69 -1.1 1,042,157 - SMc00094 0.69 -0.1 1,042,205 + SMc00094 0.72 -0.5 1,042,206 - SMc00094 0.72 -2.2 1,042,207 + SMc00094 0.72 -0.1 1,042,207 + SMc00094 0.72 -1.7 1,042,232 + SMc00094 0.74 -0.9 1,042,234 + SMc00094 0.74 -1.0 1,042,234 + SMc00094 0.74 -0.7 1,042,234 + SMc00094 0.74 -0.7 1,042,235 - SMc00094 0.74 +0.0 1,042,235 - SMc00094 0.74 -0.1 1,042,535 - -0.6 1,042,535 - -0.7 1,042,573 + -0.2 1,042,574 - -0.1 1,042,574 - -0.5 1,042,574 - -1.0 1,042,683 - -1.4 1,042,724 + +0.4 1,042,724 + -0.6 1,042,725 - -0.4 1,042,727 + +0.2 1,042,727 + -0.8 1,042,727 + +0.3 1,042,751 + -0.4 1,042,752 - +0.8 1,042,890 + SMc00127 0.18 +2.1 1,042,891 - SMc00127 0.18 -2.0 1,042,946 + SMc00127 0.21 -0.7 1,042,947 - SMc00127 0.21 -0.8 1,042,952 + SMc00127 0.22 +0.2 1,042,953 - SMc00127 0.22 +0.2 1,042,953 - SMc00127 0.22 -1.1 1,042,991 + SMc00127 0.24 +0.1 1,042,991 + SMc00127 0.24 -0.7 1,042,991 + SMc00127 0.24 -1.2 1,042,991 + SMc00127 0.24 -0.8 1,042,992 - SMc00127 0.24 -0.5 1,042,992 - SMc00127 0.24 -1.0 1,042,992 - SMc00127 0.24 -1.2 1,043,009 + SMc00127 0.25 -0.3 1,043,010 - SMc00127 0.25 +0.4 1,043,361 + SMc00127 0.48 -1.0 1,043,362 - SMc00127 0.48 -0.1 1,043,517 - SMc00127 0.58 +0.4 1,043,546 + SMc00127 0.60 -0.6 1,043,547 - SMc00127 0.60 -1.6 1,043,666 + SMc00127 0.68 -0.6 1,043,666 + SMc00127 0.68 -0.4 1,043,667 - SMc00127 0.68 -0.3 1,043,667 - SMc00127 0.68 -0.5 1,043,789 + SMc00127 0.76 -2.1 1,043,790 - SMc00127 0.76 +0.3 1,043,870 + SMc00127 0.81 -2.2 1,043,870 + SMc00127 0.81 -0.9 1,043,871 - SMc00127 0.81 +0.2 1,043,900 + SMc00127 0.83 +1.1 1,043,900 + SMc00127 0.83 -0.6 1,043,900 + SMc00127 0.83 -0.4 1,043,900 + SMc00127 0.83 -0.5 1,043,901 - SMc00127 0.83 -0.7 1,043,901 - SMc00127 0.83 -0.9 1,043,901 - SMc00127 0.83 +0.9 1,043,901 - SMc00127 0.83 -0.7 1,043,901 - SMc00127 0.83 -1.2 1,043,901 - SMc00127 0.83 -0.8 1,043,921 + SMc00127 0.85 +0.1 1,044,014 + -0.8 1,044,014 + -1.3 1,044,015 - +0.4 1,044,144 + -1.0 1,044,312 + +0.2 1,044,313 - +0.3 1,044,324 + -0.7 1,044,381 + -0.3 1,044,447 + SMc00095 0.20 -0.3 1,044,447 + SMc00095 0.20 -0.5 1,044,448 - SMc00095 0.20 +2.7 1,044,448 - SMc00095 0.20 +0.6 1,044,556 - SMc00095 0.38 -0.4 1,044,556 - SMc00095 0.38 -1.3 1,044,603 + SMc00095 0.46 -1.2 1,044,604 - SMc00095 0.46 +1.0 1,044,604 - SMc00095 0.46 +0.4 1,044,856 + SMc00095 0.87 -0.3 1,044,959 + +1.1
Or see this region's nucleotide sequence