Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0647 and SMa0649 overlap by 20 nucleotides SMa0649 and SMa5027 are separated by 589 nucleotides SMa5027 and SMa0656 are separated by 396 nucleotides
SMa0647: SMa0647 - hypothetical protein, at 346,110 to 346,361
SMa0647
SMa0649: SMa0649 - hypothetical protein, at 346,342 to 346,659
SMa0649
SMa5027: SMa5027 - hypothetical protein, at 347,249 to 347,851
SMa5027
SMa0656: SMa0656 - TRm24 transposase, at 348,248 to 349,249
SMa0656
Position (kb) 347
348 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 346.701 kb on + strand at 346.832 kb on + strand at 346.833 kb on - strand at 346.833 kb on - strand at 346.850 kb on - strand at 346.915 kb on + strand at 347.223 kb on + strand at 347.224 kb on - strand at 347.229 kb on + strand at 347.320 kb on + strand, within SMa5027 at 347.320 kb on + strand, within SMa5027 at 347.321 kb on - strand, within SMa5027 at 347.612 kb on + strand, within SMa5027 at 347.613 kb on - strand, within SMa5027 at 347.836 kb on + strand at 347.836 kb on + strand at 347.837 kb on - strand at 348.094 kb on + strand at 348.095 kb on - strand at 348.170 kb on + strand at 348.171 kb on - strand at 348.171 kb on - strand at 348.171 kb on - strand at 348.171 kb on - strand at 348.172 kb on + strand at 348.172 kb on + strand at 348.173 kb on - strand at 348.173 kb on - strand at 348.173 kb on - strand at 348.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 346,701 + +1.2 346,832 + -0.3 346,833 - +0.0 346,833 - -0.2 346,850 - -1.8 346,915 + -0.6 347,223 + +0.6 347,224 - -0.2 347,229 + +1.2 347,320 + SMa5027 0.12 +0.0 347,320 + SMa5027 0.12 -0.1 347,321 - SMa5027 0.12 -0.7 347,612 + SMa5027 0.60 -1.6 347,613 - SMa5027 0.60 -0.9 347,836 + -1.5 347,836 + +0.5 347,837 - -0.0 348,094 + +2.9 348,095 - -0.0 348,170 + +0.8 348,171 - +0.4 348,171 - -0.1 348,171 - +0.3 348,171 - -0.2 348,172 + -0.7 348,172 + -0.4 348,173 - +0.5 348,173 - +0.5 348,173 - +0.4 348,176 - +0.1
Or see this region's nucleotide sequence