Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0929 and SMa0930 overlap by 10 nucleotides SMa0930 and SMa0933 overlap by 11 nucleotides SMa0933 and SMa0934 are separated by 178 nucleotides SMa0934 and SMa0937 are separated by 448 nucleotides
SMa0929: SMa0929 - conjugal transfer protein TraG, at 513,088 to 515,007
SMa0929
SMa0930: SMa0930 - TraD conjugal transfer protein, at 514,998 to 515,237
SMa0930
SMa0933: SMa0933 - TraC conjugal transfer protein, at 515,227 to 515,514
SMa0933
SMa0934: SMa0934 - TraA1 conjugal transfer protein, at 515,693 to 520,312
SMa0934
SMa0937: SMa0937 - hypothetical protein, at 520,761 to 522,920
SMa0937
Position (kb) 515
516
517
518
519
520
521 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 514.931 kb on + strand at 514.931 kb on + strand at 514.932 kb on - strand at 515.438 kb on - strand, within SMa0933 at 515.547 kb on + strand at 515.651 kb on - strand at 515.651 kb on - strand at 515.651 kb on - strand at 515.651 kb on - strand at 515.651 kb on - strand at 515.651 kb on - strand at 516.095 kb on + strand at 516.095 kb on + strand at 516.569 kb on - strand, within SMa0934 at 516.898 kb on + strand, within SMa0934 at 517.395 kb on - strand, within SMa0934 at 518.047 kb on + strand, within SMa0934 at 518.147 kb on + strand, within SMa0934 at 518.216 kb on - strand, within SMa0934 at 519.261 kb on - strand, within SMa0934 at 519.757 kb on + strand, within SMa0934 at 519.758 kb on - strand, within SMa0934 at 520.317 kb on - strand at 520.317 kb on - strand at 520.364 kb on - strand at 520.364 kb on - strand at 520.442 kb on + strand at 520.443 kb on - strand at 520.610 kb on + strand at 520.610 kb on + strand at 520.610 kb on + strand at 520.612 kb on + strand at 520.612 kb on + strand at 520.612 kb on + strand at 520.645 kb on + strand at 520.762 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 514,931 + -1.8 514,931 + +1.3 514,932 - +2.7 515,438 - SMa0933 0.73 -0.2 515,547 + -0.1 515,651 - +2.0 515,651 - +1.7 515,651 - -0.4 515,651 - +0.7 515,651 - -0.5 515,651 - -0.1 516,095 + -0.2 516,095 + -0.2 516,569 - SMa0934 0.19 -0.2 516,898 + SMa0934 0.26 -1.3 517,395 - SMa0934 0.37 -2.3 518,047 + SMa0934 0.51 -2.2 518,147 + SMa0934 0.53 -0.7 518,216 - SMa0934 0.55 -0.4 519,261 - SMa0934 0.77 -0.0 519,757 + SMa0934 0.88 -0.1 519,758 - SMa0934 0.88 -2.5 520,317 - +0.0 520,317 - -0.9 520,364 - +0.2 520,364 - -1.4 520,442 + -0.8 520,443 - -0.3 520,610 + +0.3 520,610 + -0.4 520,610 + +0.7 520,612 + -0.5 520,612 + -0.6 520,612 + -0.6 520,645 + -2.8 520,762 + -0.8
Or see this region's nucleotide sequence