Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21231 and SM_b21232 are separated by 256 nucleotides SM_b21232 and SM_b21233 are separated by 2 nucleotides SM_b21233 and SM_b21234 overlap by 4 nucleotides
SM_b21231: SM_b21231 - glycosyltransferase, at 795,654 to 796,808
_b21231
SM_b21232: SM_b21232 - nucleotide sugar epimerase dehydratase, at 797,065 to 799,167
_b21232
SM_b21233: SM_b21233 - transposase of insertion sequence ISRm1 orfB protein, at 799,170 to 800,000
_b21233
SM_b21234: SM_b21234 - transposase of insertion sequence ISRm1 orfA protein, at 799,997 to 800,389
_b21234
Position (kb) 797
798
799
800 Strain fitness (log2 ratio) -2
-1
0
1 at 796.141 kb on + strand, within SM_b21231 at 796.142 kb on - strand, within SM_b21231 at 796.321 kb on + strand, within SM_b21231 at 796.322 kb on - strand, within SM_b21231 at 796.361 kb on - strand, within SM_b21231 at 796.379 kb on - strand, within SM_b21231 at 796.379 kb on - strand, within SM_b21231 at 796.426 kb on - strand, within SM_b21231 at 796.426 kb on - strand, within SM_b21231 at 796.428 kb on - strand, within SM_b21231 at 796.428 kb on - strand, within SM_b21231 at 796.495 kb on + strand, within SM_b21231 at 796.495 kb on + strand, within SM_b21231 at 796.495 kb on + strand, within SM_b21231 at 796.495 kb on + strand, within SM_b21231 at 796.496 kb on - strand, within SM_b21231 at 796.496 kb on - strand, within SM_b21231 at 796.496 kb on - strand, within SM_b21231 at 796.496 kb on - strand, within SM_b21231 at 796.514 kb on + strand, within SM_b21231 at 796.514 kb on + strand, within SM_b21231 at 796.516 kb on + strand, within SM_b21231 at 796.517 kb on - strand, within SM_b21231 at 796.517 kb on - strand, within SM_b21231 at 796.517 kb on - strand, within SM_b21231 at 796.689 kb on + strand, within SM_b21231 at 796.920 kb on + strand at 796.920 kb on + strand at 796.920 kb on + strand at 796.921 kb on - strand at 796.956 kb on - strand at 797.055 kb on + strand at 797.055 kb on + strand at 797.546 kb on + strand, within SM_b21232 at 797.607 kb on + strand, within SM_b21232 at 797.608 kb on - strand, within SM_b21232 at 797.765 kb on + strand, within SM_b21232 at 797.765 kb on + strand, within SM_b21232 at 797.766 kb on - strand, within SM_b21232 at 797.766 kb on - strand, within SM_b21232 at 798.032 kb on + strand, within SM_b21232 at 798.086 kb on + strand, within SM_b21232 at 798.095 kb on + strand, within SM_b21232 at 798.095 kb on + strand, within SM_b21232 at 798.096 kb on - strand, within SM_b21232 at 798.096 kb on - strand, within SM_b21232 at 798.564 kb on + strand, within SM_b21232 at 798.566 kb on + strand, within SM_b21232 at 798.566 kb on + strand, within SM_b21232 at 798.602 kb on + strand, within SM_b21232 at 798.602 kb on + strand, within SM_b21232 at 798.603 kb on - strand, within SM_b21232 at 798.603 kb on - strand, within SM_b21232 at 798.890 kb on + strand, within SM_b21232 at 798.909 kb on - strand, within SM_b21232 at 798.940 kb on + strand, within SM_b21232 at 798.940 kb on + strand, within SM_b21232 at 798.941 kb on - strand, within SM_b21232 at 798.950 kb on + strand, within SM_b21232 at 799.016 kb on + strand at 799.021 kb on + strand at 799.022 kb on - strand at 799.073 kb on + strand at 799.073 kb on + strand at 799.114 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 796,141 + SM_b21231 0.42 -0.3 796,142 - SM_b21231 0.42 +0.3 796,321 + SM_b21231 0.58 +0.5 796,322 - SM_b21231 0.58 +0.6 796,361 - SM_b21231 0.61 -0.4 796,379 - SM_b21231 0.63 -0.8 796,379 - SM_b21231 0.63 +0.4 796,426 - SM_b21231 0.67 -0.4 796,426 - SM_b21231 0.67 +0.0 796,428 - SM_b21231 0.67 -1.4 796,428 - SM_b21231 0.67 +0.6 796,495 + SM_b21231 0.73 -0.2 796,495 + SM_b21231 0.73 -1.2 796,495 + SM_b21231 0.73 -0.1 796,495 + SM_b21231 0.73 +1.0 796,496 - SM_b21231 0.73 -0.0 796,496 - SM_b21231 0.73 -0.2 796,496 - SM_b21231 0.73 -1.2 796,496 - SM_b21231 0.73 +0.9 796,514 + SM_b21231 0.74 +0.8 796,514 + SM_b21231 0.74 -0.2 796,516 + SM_b21231 0.75 +0.0 796,517 - SM_b21231 0.75 -1.9 796,517 - SM_b21231 0.75 +0.7 796,517 - SM_b21231 0.75 -0.3 796,689 + SM_b21231 0.90 +0.3 796,920 + +0.2 796,920 + -0.3 796,920 + +0.4 796,921 - -0.3 796,956 - +0.0 797,055 + -0.1 797,055 + +1.6 797,546 + SM_b21232 0.23 -1.0 797,607 + SM_b21232 0.26 -0.7 797,608 - SM_b21232 0.26 +0.3 797,765 + SM_b21232 0.33 -0.0 797,765 + SM_b21232 0.33 -1.5 797,766 - SM_b21232 0.33 -0.5 797,766 - SM_b21232 0.33 -0.6 798,032 + SM_b21232 0.46 +0.9 798,086 + SM_b21232 0.49 +0.1 798,095 + SM_b21232 0.49 +0.3 798,095 + SM_b21232 0.49 -0.6 798,096 - SM_b21232 0.49 -0.5 798,096 - SM_b21232 0.49 +0.5 798,564 + SM_b21232 0.71 +0.7 798,566 + SM_b21232 0.71 -1.0 798,566 + SM_b21232 0.71 +0.7 798,602 + SM_b21232 0.73 -0.3 798,602 + SM_b21232 0.73 +0.2 798,603 - SM_b21232 0.73 +0.7 798,603 - SM_b21232 0.73 -0.6 798,890 + SM_b21232 0.87 -0.1 798,909 - SM_b21232 0.88 -0.2 798,940 + SM_b21232 0.89 +0.2 798,940 + SM_b21232 0.89 -0.1 798,941 - SM_b21232 0.89 -1.3 798,950 + SM_b21232 0.90 +0.0 799,016 + +0.8 799,021 + +0.2 799,022 - -0.6 799,073 + -1.6 799,073 + -1.1 799,114 + -0.5
Or see this region's nucleotide sequence