Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b21117 and SM_b21119 are separated by 250 nucleotides SM_b21119 and SM_b21120 overlap by 23 nucleotides SM_b21120 and SM_b21121 overlap by 8 nucleotides
SM_b21117: SM_b21117 - transcriptional regulator, at 758,135 to 758,593
_b21117
SM_b21119: SM_b21119 - gluconokinase, at 758,844 to 759,380
_b21119
SM_b21120: SM_b21120 - hypothetical protein, at 759,358 to 759,576
_b21120
SM_b21121: SM_b21121 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) <small>(from data)</small>, at 759,569 to 760,732
_b21121
Position (kb) 759
760 Strain fitness (log2 ratio) -2
-1
0
1
2 at 758.405 kb on + strand, within SM_b21117 at 758.405 kb on + strand, within SM_b21117 at 758.406 kb on - strand, within SM_b21117 at 758.406 kb on - strand, within SM_b21117 at 758.406 kb on - strand, within SM_b21117 at 758.406 kb on - strand, within SM_b21117 at 758.406 kb on - strand, within SM_b21117 at 758.522 kb on + strand, within SM_b21117 at 758.522 kb on + strand, within SM_b21117 at 758.522 kb on + strand, within SM_b21117 at 758.523 kb on - strand, within SM_b21117 at 758.523 kb on - strand, within SM_b21117 at 758.576 kb on - strand at 758.598 kb on + strand at 758.599 kb on - strand at 758.599 kb on - strand at 758.625 kb on + strand at 758.625 kb on + strand at 758.626 kb on - strand at 758.632 kb on + strand at 758.634 kb on + strand at 758.634 kb on + strand at 758.635 kb on - strand at 758.706 kb on + strand at 758.849 kb on + strand at 758.849 kb on + strand at 758.850 kb on - strand at 758.850 kb on - strand at 758.857 kb on - strand at 759.301 kb on + strand, within SM_b21119 at 759.301 kb on + strand, within SM_b21119 at 759.301 kb on + strand, within SM_b21119 at 759.301 kb on + strand, within SM_b21119 at 759.301 kb on + strand, within SM_b21119 at 759.302 kb on - strand, within SM_b21119 at 759.469 kb on - strand, within SM_b21120 at 759.469 kb on - strand, within SM_b21120 at 759.469 kb on - strand, within SM_b21120 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.811 kb on - strand, within SM_b21121 at 759.843 kb on - strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.911 kb on + strand, within SM_b21121 at 759.912 kb on - strand, within SM_b21121 at 760.025 kb on + strand, within SM_b21121 at 760.026 kb on - strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.088 kb on + strand, within SM_b21121 at 760.089 kb on - strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.288 kb on + strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.289 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.374 kb on - strand, within SM_b21121 at 760.448 kb on + strand at 760.448 kb on + strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.449 kb on - strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.475 kb on + strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.476 kb on - strand, within SM_b21121 at 760.481 kb on + strand, within SM_b21121 at 760.540 kb on + strand, within SM_b21121
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 758,405 + SM_b21117 0.59 -0.7 758,405 + SM_b21117 0.59 +0.4 758,406 - SM_b21117 0.59 -0.9 758,406 - SM_b21117 0.59 -0.7 758,406 - SM_b21117 0.59 -0.5 758,406 - SM_b21117 0.59 +0.4 758,406 - SM_b21117 0.59 +0.7 758,522 + SM_b21117 0.84 +1.3 758,522 + SM_b21117 0.84 +1.0 758,522 + SM_b21117 0.84 +0.4 758,523 - SM_b21117 0.85 +0.3 758,523 - SM_b21117 0.85 -0.5 758,576 - +0.4 758,598 + -0.7 758,599 - -0.1 758,599 - -0.1 758,625 + +0.9 758,625 + +0.0 758,626 - -1.9 758,632 + +0.1 758,634 + -0.1 758,634 + +1.4 758,635 - -0.2 758,706 + +0.1 758,849 + +0.9 758,849 + -0.0 758,850 - -0.2 758,850 - +1.5 758,857 - +2.2 759,301 + SM_b21119 0.85 +0.2 759,301 + SM_b21119 0.85 -0.7 759,301 + SM_b21119 0.85 +0.4 759,301 + SM_b21119 0.85 +0.2 759,301 + SM_b21119 0.85 +0.0 759,302 - SM_b21119 0.85 +0.1 759,469 - SM_b21120 0.51 -1.0 759,469 - SM_b21120 0.51 -0.8 759,469 - SM_b21120 0.51 -0.4 759,811 - SM_b21121 0.21 -0.7 759,811 - SM_b21121 0.21 -0.4 759,811 - SM_b21121 0.21 +0.9 759,843 - SM_b21121 0.24 -0.2 759,911 + SM_b21121 0.29 -2.0 759,911 + SM_b21121 0.29 -0.7 759,911 + SM_b21121 0.29 +0.6 759,912 - SM_b21121 0.29 -0.0 760,025 + SM_b21121 0.39 -0.5 760,026 - SM_b21121 0.39 -0.8 760,088 + SM_b21121 0.45 -0.3 760,088 + SM_b21121 0.45 -0.7 760,089 - SM_b21121 0.45 -1.2 760,288 + SM_b21121 0.62 -1.3 760,288 + SM_b21121 0.62 +0.2 760,288 + SM_b21121 0.62 -0.5 760,288 + SM_b21121 0.62 -0.5 760,288 + SM_b21121 0.62 -0.3 760,289 - SM_b21121 0.62 -0.0 760,289 - SM_b21121 0.62 +0.4 760,374 - SM_b21121 0.69 +0.2 760,374 - SM_b21121 0.69 -0.9 760,374 - SM_b21121 0.69 +0.3 760,448 + +1.8 760,448 + SM_b21121 0.76 +0.2 760,449 - SM_b21121 0.76 -0.2 760,449 - SM_b21121 0.76 +0.2 760,449 - SM_b21121 0.76 +0.2 760,449 - SM_b21121 0.76 -1.1 760,449 - SM_b21121 0.76 -0.0 760,449 - SM_b21121 0.76 -0.8 760,475 + SM_b21121 0.78 -0.2 760,475 + SM_b21121 0.78 +0.8 760,476 - SM_b21121 0.78 +0.1 760,476 - SM_b21121 0.78 -0.5 760,476 - SM_b21121 0.78 +0.8 760,476 - SM_b21121 0.78 -0.5 760,481 + SM_b21121 0.78 -0.5 760,540 + SM_b21121 0.83 -0.2
Or see this region's nucleotide sequence