Experiment: LB, pH6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20002 and SM_b20003 are separated by 58 nucleotides SM_b20003 and SM_b20004 are separated by 6 nucleotides SM_b20004 and SM_b20005 are separated by 115 nucleotides SM_b20005 and SM_b20006 are separated by 185 nucleotides
SM_b20002: SM_b20002 - ABC transporter for Lactose, ATPase component (from data) , at 5,350 to 6,426
_b20002
SM_b20003: SM_b20003 - pyrroline-5-carboxylate reductase, at 6,485 to 7,261
_b20003
SM_b20004: SM_b20004 - gstR-like transcriptional regulator protein, at 7,268 to 8,173
_b20004
SM_b20005: SM_b20005 - glutathione S-transferase, at 8,289 to 8,900
_b20005
SM_b20006: SM_b20006 - hypothetical protein, at 9,086 to 10,039
_b20006
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-1
0
1 at 6.281 kb on - strand, within SM_b20002 at 6.448 kb on + strand at 6.448 kb on + strand at 6.449 kb on - strand at 6.449 kb on - strand at 6.449 kb on - strand at 6.449 kb on - strand at 6.490 kb on + strand at 6.947 kb on + strand, within SM_b20003 at 6.948 kb on - strand, within SM_b20003 at 6.986 kb on + strand, within SM_b20003 at 6.986 kb on + strand, within SM_b20003 at 6.987 kb on - strand, within SM_b20003 at 7.059 kb on - strand, within SM_b20003 at 7.059 kb on - strand, within SM_b20003 at 7.549 kb on + strand, within SM_b20004 at 7.806 kb on - strand, within SM_b20004 at 7.813 kb on + strand, within SM_b20004 at 7.980 kb on + strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 7.981 kb on - strand, within SM_b20004 at 8.092 kb on + strand at 8.093 kb on - strand at 8.093 kb on - strand at 8.298 kb on + strand at 8.298 kb on + strand at 8.298 kb on + strand at 8.299 kb on - strand at 8.499 kb on + strand, within SM_b20005 at 8.764 kb on - strand, within SM_b20005 at 8.770 kb on - strand, within SM_b20005 at 8.774 kb on - strand, within SM_b20005 at 8.812 kb on - strand, within SM_b20005
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH6 remove 6,281 - SM_b20002 0.86 -0.1 6,448 + -0.4 6,448 + +0.9 6,449 - +0.4 6,449 - +1.4 6,449 - -0.1 6,449 - -0.1 6,490 + -0.4 6,947 + SM_b20003 0.59 +0.9 6,948 - SM_b20003 0.60 -1.0 6,986 + SM_b20003 0.64 +0.7 6,986 + SM_b20003 0.64 -0.2 6,987 - SM_b20003 0.65 -0.4 7,059 - SM_b20003 0.74 +0.2 7,059 - SM_b20003 0.74 +1.1 7,549 + SM_b20004 0.31 -0.4 7,806 - SM_b20004 0.59 -0.1 7,813 + SM_b20004 0.60 -0.5 7,980 + SM_b20004 0.79 -0.4 7,981 - SM_b20004 0.79 -0.5 7,981 - SM_b20004 0.79 -0.3 7,981 - SM_b20004 0.79 -0.4 7,981 - SM_b20004 0.79 -0.1 8,092 + -0.9 8,093 - -0.6 8,093 - +0.1 8,298 + +0.2 8,298 + +1.1 8,298 + -0.2 8,299 - +0.8 8,499 + SM_b20005 0.34 +0.0 8,764 - SM_b20005 0.78 -0.2 8,770 - SM_b20005 0.79 +0.5 8,774 - SM_b20005 0.79 -0.8 8,812 - SM_b20005 0.85 -0.1
Or see this region's nucleotide sequence