Experiment: LB, pH7
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30330 and BPHYT_RS30335 are separated by 11 nucleotides BPHYT_RS30335 and BPHYT_RS30340 are separated by 25 nucleotides BPHYT_RS30340 and BPHYT_RS30345 are separated by 20 nucleotides BPHYT_RS30345 and BPHYT_RS30350 are separated by 222 nucleotides
BPHYT_RS30330: BPHYT_RS30330 - hypothetical protein, at 2,360,135 to 2,360,803
_RS30330
BPHYT_RS30335: BPHYT_RS30335 - hypothetical protein, at 2,360,815 to 2,361,213
_RS30335
BPHYT_RS30340: BPHYT_RS30340 - AraC family transcriptional regulator, at 2,361,239 to 2,362,093
_RS30340
BPHYT_RS30345: BPHYT_RS30345 - alanyl-tRNA synthetase, at 2,362,114 to 2,362,827
_RS30345
BPHYT_RS30350: BPHYT_RS30350 - LysR family transcriptional regulator, at 2,363,050 to 2,363,970
_RS30350
Position (kb)
2361
2362
2363 Strain fitness (log2 ratio)
-1
0
1 at 2360.558 kb on + strand, within BPHYT_RS30330 at 2360.611 kb on - strand, within BPHYT_RS30330 at 2361.031 kb on + strand, within BPHYT_RS30335 at 2361.032 kb on - strand, within BPHYT_RS30335 at 2361.460 kb on - strand, within BPHYT_RS30340 at 2361.817 kb on - strand, within BPHYT_RS30340 at 2362.079 kb on - strand at 2362.347 kb on - strand, within BPHYT_RS30345 at 2362.506 kb on - strand, within BPHYT_RS30345 at 2362.536 kb on + strand, within BPHYT_RS30345 at 2362.857 kb on - strand at 2362.984 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH7 remove 2,360,558 + BPHYT_RS30330 0.63 +0.6 2,360,611 - BPHYT_RS30330 0.71 -1.1 2,361,031 + BPHYT_RS30335 0.54 +0.2 2,361,032 - BPHYT_RS30335 0.54 +0.3 2,361,460 - BPHYT_RS30340 0.26 +0.0 2,361,817 - BPHYT_RS30340 0.68 -1.0 2,362,079 - -0.8 2,362,347 - BPHYT_RS30345 0.33 +0.0 2,362,506 - BPHYT_RS30345 0.55 -0.1 2,362,536 + BPHYT_RS30345 0.59 -0.1 2,362,857 - +0.4 2,362,984 - +1.3
Or see this region's nucleotide sequence