Experiment: CDM1 without Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS20850 and BBR_RS14740 are separated by 179 nucleotides BBR_RS14740 and BBR_RS20855 are separated by 278 nucleotides BBR_RS20855 and BBR_RS14745 are separated by 45 nucleotides
BBR_RS20850: BBR_RS20850 - resolvase, at 1,080,681 to 1,080,923
_RS20850
BBR_RS14740: BBR_RS14740 - YccF domain-containing protein, at 1,081,103 to 1,081,468
_RS14740
BBR_RS20855: BBR_RS20855 - hypothetical protein, at 1,081,747 to 1,082,028
_RS20855
BBR_RS14745: BBR_RS14745 - MFS transporter, at 1,082,074 to 1,084,125
_RS14745
Position (kb)
1081
1082
1083 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1080.864 kb on - strand, within BBR_RS20850 at 1080.901 kb on + strand at 1080.905 kb on + strand at 1080.914 kb on + strand at 1080.927 kb on - strand at 1080.932 kb on - strand at 1080.982 kb on - strand at 1081.092 kb on - strand at 1081.189 kb on + strand, within BBR_RS14740 at 1081.223 kb on + strand, within BBR_RS14740 at 1081.231 kb on - strand, within BBR_RS14740 at 1081.268 kb on + strand, within BBR_RS14740 at 1081.271 kb on - strand, within BBR_RS14740 at 1081.276 kb on - strand, within BBR_RS14740 at 1081.348 kb on + strand, within BBR_RS14740 at 1081.348 kb on + strand, within BBR_RS14740 at 1081.395 kb on - strand, within BBR_RS14740 at 1081.421 kb on + strand, within BBR_RS14740 at 1081.429 kb on - strand, within BBR_RS14740 at 1081.544 kb on + strand at 1081.558 kb on - strand at 1081.564 kb on - strand at 1081.609 kb on + strand at 1081.661 kb on - strand at 1081.731 kb on + strand at 1081.739 kb on + strand at 1081.739 kb on + strand at 1081.768 kb on + strand at 1081.776 kb on - strand, within BBR_RS20855 at 1081.791 kb on + strand, within BBR_RS20855 at 1081.846 kb on - strand, within BBR_RS20855 at 1081.865 kb on + strand, within BBR_RS20855 at 1081.871 kb on - strand, within BBR_RS20855 at 1081.916 kb on + strand, within BBR_RS20855 at 1082.007 kb on + strand at 1082.052 kb on - strand at 1082.134 kb on - strand at 1082.139 kb on + strand at 1082.224 kb on + strand at 1082.227 kb on - strand at 1082.250 kb on + strand at 1082.274 kb on - strand at 1082.284 kb on - strand, within BBR_RS14745 at 1082.380 kb on + strand, within BBR_RS14745 at 1082.380 kb on - strand, within BBR_RS14745 at 1082.384 kb on + strand, within BBR_RS14745 at 1082.387 kb on - strand, within BBR_RS14745 at 1082.389 kb on + strand, within BBR_RS14745 at 1082.389 kb on + strand, within BBR_RS14745 at 1082.391 kb on + strand, within BBR_RS14745 at 1082.399 kb on - strand, within BBR_RS14745 at 1082.440 kb on - strand, within BBR_RS14745 at 1082.488 kb on - strand, within BBR_RS14745 at 1082.544 kb on + strand, within BBR_RS14745 at 1082.570 kb on + strand, within BBR_RS14745 at 1082.575 kb on + strand, within BBR_RS14745 at 1082.592 kb on - strand, within BBR_RS14745 at 1082.646 kb on + strand, within BBR_RS14745 at 1082.646 kb on + strand, within BBR_RS14745 at 1082.651 kb on + strand, within BBR_RS14745 at 1082.681 kb on - strand, within BBR_RS14745 at 1082.762 kb on - strand, within BBR_RS14745 at 1082.762 kb on - strand, within BBR_RS14745 at 1082.762 kb on - strand, within BBR_RS14745 at 1082.762 kb on - strand, within BBR_RS14745 at 1082.785 kb on + strand, within BBR_RS14745 at 1082.785 kb on + strand, within BBR_RS14745 at 1082.793 kb on - strand, within BBR_RS14745 at 1082.835 kb on - strand, within BBR_RS14745 at 1082.876 kb on - strand, within BBR_RS14745 at 1082.883 kb on - strand, within BBR_RS14745 at 1082.921 kb on + strand, within BBR_RS14745 at 1082.934 kb on - strand, within BBR_RS14745 at 1082.940 kb on + strand, within BBR_RS14745 at 1082.943 kb on - strand, within BBR_RS14745 at 1082.947 kb on + strand, within BBR_RS14745 at 1083.000 kb on + strand, within BBR_RS14745
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Threonine remove 1,080,864 - BBR_RS20850 0.75 -0.8 1,080,901 + -1.2 1,080,905 + +0.6 1,080,914 + -1.8 1,080,927 - -0.4 1,080,932 - -2.5 1,080,982 - +0.4 1,081,092 - -0.6 1,081,189 + BBR_RS14740 0.23 -0.0 1,081,223 + BBR_RS14740 0.33 -0.2 1,081,231 - BBR_RS14740 0.35 +0.6 1,081,268 + BBR_RS14740 0.45 -1.0 1,081,271 - BBR_RS14740 0.46 +2.7 1,081,276 - BBR_RS14740 0.47 -0.2 1,081,348 + BBR_RS14740 0.67 -3.3 1,081,348 + BBR_RS14740 0.67 -1.0 1,081,395 - BBR_RS14740 0.80 -0.8 1,081,421 + BBR_RS14740 0.87 -0.1 1,081,429 - BBR_RS14740 0.89 -0.2 1,081,544 + -1.4 1,081,558 - +1.1 1,081,564 - +0.5 1,081,609 + -0.9 1,081,661 - -2.6 1,081,731 + -1.9 1,081,739 + +1.6 1,081,739 + +0.1 1,081,768 + +0.4 1,081,776 - BBR_RS20855 0.10 -2.9 1,081,791 + BBR_RS20855 0.16 -1.5 1,081,846 - BBR_RS20855 0.35 -1.3 1,081,865 + BBR_RS20855 0.42 +0.3 1,081,871 - BBR_RS20855 0.44 -3.0 1,081,916 + BBR_RS20855 0.60 -1.6 1,082,007 + +0.3 1,082,052 - +0.8 1,082,134 - +0.3 1,082,139 + -0.5 1,082,224 + +1.9 1,082,227 - +0.7 1,082,250 + -1.9 1,082,274 - +0.4 1,082,284 - BBR_RS14745 0.10 -0.0 1,082,380 + BBR_RS14745 0.15 +0.4 1,082,380 - BBR_RS14745 0.15 -1.1 1,082,384 + BBR_RS14745 0.15 -0.2 1,082,387 - BBR_RS14745 0.15 -1.2 1,082,389 + BBR_RS14745 0.15 +0.6 1,082,389 + BBR_RS14745 0.15 -0.3 1,082,391 + BBR_RS14745 0.15 +0.8 1,082,399 - BBR_RS14745 0.16 +0.2 1,082,440 - BBR_RS14745 0.18 +0.5 1,082,488 - BBR_RS14745 0.20 -1.0 1,082,544 + BBR_RS14745 0.23 -0.1 1,082,570 + BBR_RS14745 0.24 -1.9 1,082,575 + BBR_RS14745 0.24 -0.3 1,082,592 - BBR_RS14745 0.25 +1.0 1,082,646 + BBR_RS14745 0.28 +0.3 1,082,646 + BBR_RS14745 0.28 -2.4 1,082,651 + BBR_RS14745 0.28 +0.3 1,082,681 - BBR_RS14745 0.30 -0.2 1,082,762 - BBR_RS14745 0.34 +0.6 1,082,762 - BBR_RS14745 0.34 +0.8 1,082,762 - BBR_RS14745 0.34 +0.0 1,082,762 - BBR_RS14745 0.34 -2.2 1,082,785 + BBR_RS14745 0.35 -1.4 1,082,785 + BBR_RS14745 0.35 -1.5 1,082,793 - BBR_RS14745 0.35 -1.3 1,082,835 - BBR_RS14745 0.37 +0.1 1,082,876 - BBR_RS14745 0.39 +0.4 1,082,883 - BBR_RS14745 0.39 -0.4 1,082,921 + BBR_RS14745 0.41 -0.6 1,082,934 - BBR_RS14745 0.42 +1.0 1,082,940 + BBR_RS14745 0.42 -0.3 1,082,943 - BBR_RS14745 0.42 -2.7 1,082,947 + BBR_RS14745 0.43 -1.3 1,083,000 + BBR_RS14745 0.45 +0.5
Or see this region's nucleotide sequence