Experiment: CDM1 without Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS16800 and BBR_RS16805 are separated by 291 nucleotides BBR_RS16805 and BBR_RS16810 are separated by 134 nucleotides
BBR_RS16800: BBR_RS16800 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 1,538,316 to 1,539,308
_RS16800
BBR_RS16805: BBR_RS16805 - aldose 1-epimerase, at 1,539,600 to 1,540,556
_RS16805
BBR_RS16810: BBR_RS16810 - aldose 1-epimerase, at 1,540,691 to 1,541,635
_RS16810
Position (kb)
1539
1540
1541 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1539.331 kb on + strand at 1539.339 kb on - strand at 1539.341 kb on + strand at 1539.355 kb on + strand at 1539.361 kb on - strand at 1539.389 kb on - strand at 1539.438 kb on + strand at 1539.446 kb on - strand at 1539.446 kb on - strand at 1539.478 kb on - strand at 1539.502 kb on - strand at 1539.541 kb on - strand at 1539.546 kb on - strand at 1539.546 kb on - strand at 1539.584 kb on + strand at 1539.748 kb on + strand, within BBR_RS16805 at 1539.827 kb on + strand, within BBR_RS16805 at 1539.832 kb on - strand, within BBR_RS16805 at 1539.835 kb on - strand, within BBR_RS16805 at 1539.839 kb on + strand, within BBR_RS16805 at 1539.861 kb on - strand, within BBR_RS16805 at 1539.890 kb on - strand, within BBR_RS16805 at 1539.890 kb on - strand, within BBR_RS16805 at 1539.952 kb on + strand, within BBR_RS16805 at 1539.960 kb on - strand, within BBR_RS16805 at 1539.960 kb on - strand, within BBR_RS16805 at 1540.038 kb on - strand, within BBR_RS16805 at 1540.065 kb on - strand, within BBR_RS16805 at 1540.066 kb on + strand, within BBR_RS16805 at 1540.072 kb on - strand, within BBR_RS16805 at 1540.117 kb on - strand, within BBR_RS16805 at 1540.130 kb on - strand, within BBR_RS16805 at 1540.168 kb on - strand, within BBR_RS16805 at 1540.168 kb on - strand, within BBR_RS16805 at 1540.168 kb on - strand, within BBR_RS16805 at 1540.168 kb on - strand, within BBR_RS16805 at 1540.174 kb on - strand, within BBR_RS16805 at 1540.205 kb on + strand, within BBR_RS16805 at 1540.205 kb on + strand, within BBR_RS16805 at 1540.205 kb on + strand, within BBR_RS16805 at 1540.218 kb on - strand, within BBR_RS16805 at 1540.265 kb on - strand, within BBR_RS16805 at 1540.280 kb on + strand, within BBR_RS16805 at 1540.298 kb on - strand, within BBR_RS16805 at 1540.298 kb on - strand, within BBR_RS16805 at 1540.298 kb on - strand, within BBR_RS16805 at 1540.298 kb on - strand, within BBR_RS16805 at 1540.319 kb on - strand, within BBR_RS16805 at 1540.344 kb on - strand, within BBR_RS16805 at 1540.344 kb on - strand, within BBR_RS16805 at 1540.391 kb on + strand, within BBR_RS16805 at 1540.399 kb on - strand, within BBR_RS16805 at 1540.399 kb on - strand, within BBR_RS16805 at 1540.428 kb on + strand, within BBR_RS16805 at 1540.436 kb on - strand, within BBR_RS16805 at 1540.454 kb on - strand, within BBR_RS16805 at 1540.463 kb on + strand at 1540.515 kb on + strand at 1540.515 kb on + strand at 1540.525 kb on + strand at 1540.530 kb on - strand at 1540.534 kb on + strand at 1540.627 kb on + strand at 1540.682 kb on + strand at 1540.682 kb on + strand at 1540.687 kb on - strand at 1540.689 kb on - strand at 1540.689 kb on - strand at 1540.697 kb on - strand at 1540.703 kb on + strand at 1540.744 kb on - strand at 1540.757 kb on + strand at 1540.773 kb on - strand at 1540.784 kb on + strand at 1540.792 kb on - strand, within BBR_RS16810 at 1540.816 kb on - strand, within BBR_RS16810 at 1540.894 kb on + strand, within BBR_RS16810 at 1540.897 kb on - strand, within BBR_RS16810 at 1540.902 kb on - strand, within BBR_RS16810 at 1540.943 kb on + strand, within BBR_RS16810 at 1540.955 kb on + strand, within BBR_RS16810 at 1540.962 kb on + strand, within BBR_RS16810 at 1540.989 kb on + strand, within BBR_RS16810 at 1541.031 kb on + strand, within BBR_RS16810 at 1541.051 kb on - strand, within BBR_RS16810 at 1541.094 kb on - strand, within BBR_RS16810 at 1541.105 kb on - strand, within BBR_RS16810 at 1541.105 kb on - strand, within BBR_RS16810 at 1541.107 kb on + strand, within BBR_RS16810 at 1541.107 kb on + strand, within BBR_RS16810 at 1541.115 kb on - strand, within BBR_RS16810 at 1541.144 kb on + strand, within BBR_RS16810 at 1541.190 kb on + strand, within BBR_RS16810 at 1541.201 kb on + strand, within BBR_RS16810 at 1541.215 kb on + strand, within BBR_RS16810 at 1541.259 kb on + strand, within BBR_RS16810 at 1541.261 kb on + strand, within BBR_RS16810 at 1541.267 kb on - strand, within BBR_RS16810 at 1541.268 kb on + strand, within BBR_RS16810 at 1541.268 kb on + strand, within BBR_RS16810 at 1541.269 kb on - strand, within BBR_RS16810 at 1541.271 kb on - strand, within BBR_RS16810 at 1541.272 kb on - strand, within BBR_RS16810 at 1541.291 kb on - strand, within BBR_RS16810 at 1541.320 kb on - strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.331 kb on + strand, within BBR_RS16810 at 1541.339 kb on - strand, within BBR_RS16810 at 1541.356 kb on + strand, within BBR_RS16810 at 1541.382 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.397 kb on + strand, within BBR_RS16810 at 1541.402 kb on + strand, within BBR_RS16810 at 1541.405 kb on + strand, within BBR_RS16810 at 1541.405 kb on - strand, within BBR_RS16810 at 1541.413 kb on - strand, within BBR_RS16810 at 1541.435 kb on + strand, within BBR_RS16810 at 1541.470 kb on + strand, within BBR_RS16810 at 1541.516 kb on - strand, within BBR_RS16810 at 1541.535 kb on - strand, within BBR_RS16810 at 1541.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Threonine remove 1,539,331 + -0.4 1,539,339 - +0.9 1,539,341 + +0.4 1,539,355 + +0.5 1,539,361 - -0.4 1,539,389 - +0.5 1,539,438 + +0.0 1,539,446 - +1.1 1,539,446 - -1.2 1,539,478 - +0.6 1,539,502 - +0.6 1,539,541 - +0.2 1,539,546 - -0.7 1,539,546 - +0.2 1,539,584 + +0.3 1,539,748 + BBR_RS16805 0.15 +1.2 1,539,827 + BBR_RS16805 0.24 -0.2 1,539,832 - BBR_RS16805 0.24 -0.2 1,539,835 - BBR_RS16805 0.25 -0.1 1,539,839 + BBR_RS16805 0.25 +0.2 1,539,861 - BBR_RS16805 0.27 +0.5 1,539,890 - BBR_RS16805 0.30 -1.0 1,539,890 - BBR_RS16805 0.30 +0.9 1,539,952 + BBR_RS16805 0.37 +0.6 1,539,960 - BBR_RS16805 0.38 +1.8 1,539,960 - BBR_RS16805 0.38 +1.0 1,540,038 - BBR_RS16805 0.46 +1.0 1,540,065 - BBR_RS16805 0.49 +1.0 1,540,066 + BBR_RS16805 0.49 +0.5 1,540,072 - BBR_RS16805 0.49 +0.9 1,540,117 - BBR_RS16805 0.54 +1.0 1,540,130 - BBR_RS16805 0.55 +0.9 1,540,168 - BBR_RS16805 0.59 -1.7 1,540,168 - BBR_RS16805 0.59 -0.1 1,540,168 - BBR_RS16805 0.59 -0.3 1,540,168 - BBR_RS16805 0.59 +1.3 1,540,174 - BBR_RS16805 0.60 +0.4 1,540,205 + BBR_RS16805 0.63 +1.1 1,540,205 + BBR_RS16805 0.63 +0.8 1,540,205 + BBR_RS16805 0.63 -0.9 1,540,218 - BBR_RS16805 0.65 +1.6 1,540,265 - BBR_RS16805 0.69 +0.7 1,540,280 + BBR_RS16805 0.71 +0.1 1,540,298 - BBR_RS16805 0.73 +0.4 1,540,298 - BBR_RS16805 0.73 +0.4 1,540,298 - BBR_RS16805 0.73 -0.9 1,540,298 - BBR_RS16805 0.73 -0.5 1,540,319 - BBR_RS16805 0.75 -0.1 1,540,344 - BBR_RS16805 0.78 +0.5 1,540,344 - BBR_RS16805 0.78 -0.2 1,540,391 + BBR_RS16805 0.83 +0.9 1,540,399 - BBR_RS16805 0.83 -0.7 1,540,399 - BBR_RS16805 0.83 -0.1 1,540,428 + BBR_RS16805 0.87 +2.0 1,540,436 - BBR_RS16805 0.87 -0.8 1,540,454 - BBR_RS16805 0.89 -0.8 1,540,463 + -0.5 1,540,515 + -0.2 1,540,515 + -0.1 1,540,525 + +1.3 1,540,530 - +2.5 1,540,534 + +1.0 1,540,627 + +1.4 1,540,682 + +0.0 1,540,682 + -1.5 1,540,687 - +0.6 1,540,689 - +0.6 1,540,689 - -0.2 1,540,697 - +0.7 1,540,703 + +1.1 1,540,744 - +0.7 1,540,757 + -0.1 1,540,773 - +0.2 1,540,784 + +1.4 1,540,792 - BBR_RS16810 0.11 +1.4 1,540,816 - BBR_RS16810 0.13 -0.4 1,540,894 + BBR_RS16810 0.21 +1.0 1,540,897 - BBR_RS16810 0.22 -0.5 1,540,902 - BBR_RS16810 0.22 +0.0 1,540,943 + BBR_RS16810 0.27 +0.3 1,540,955 + BBR_RS16810 0.28 +0.7 1,540,962 + BBR_RS16810 0.29 -0.5 1,540,989 + BBR_RS16810 0.32 -0.2 1,541,031 + BBR_RS16810 0.36 +1.2 1,541,051 - BBR_RS16810 0.38 +1.1 1,541,094 - BBR_RS16810 0.43 +1.4 1,541,105 - BBR_RS16810 0.44 -0.3 1,541,105 - BBR_RS16810 0.44 -0.9 1,541,107 + BBR_RS16810 0.44 +0.9 1,541,107 + BBR_RS16810 0.44 +1.1 1,541,115 - BBR_RS16810 0.45 -0.6 1,541,144 + BBR_RS16810 0.48 +1.2 1,541,190 + BBR_RS16810 0.53 -0.6 1,541,201 + BBR_RS16810 0.54 +0.6 1,541,215 + BBR_RS16810 0.55 +1.1 1,541,259 + BBR_RS16810 0.60 +2.4 1,541,261 + BBR_RS16810 0.60 +1.0 1,541,267 - BBR_RS16810 0.61 -2.9 1,541,268 + BBR_RS16810 0.61 +1.2 1,541,268 + BBR_RS16810 0.61 +1.6 1,541,269 - BBR_RS16810 0.61 -1.2 1,541,271 - BBR_RS16810 0.61 -1.9 1,541,272 - BBR_RS16810 0.61 +0.2 1,541,291 - BBR_RS16810 0.63 +0.0 1,541,320 - BBR_RS16810 0.67 -1.7 1,541,331 + BBR_RS16810 0.68 -0.3 1,541,331 + BBR_RS16810 0.68 +1.3 1,541,331 + BBR_RS16810 0.68 +1.2 1,541,331 + BBR_RS16810 0.68 +1.1 1,541,331 + BBR_RS16810 0.68 +0.7 1,541,331 + BBR_RS16810 0.68 +1.3 1,541,331 + BBR_RS16810 0.68 +0.6 1,541,331 + BBR_RS16810 0.68 +0.2 1,541,339 - BBR_RS16810 0.69 +1.7 1,541,356 + BBR_RS16810 0.70 -0.5 1,541,382 + BBR_RS16810 0.73 -0.7 1,541,397 + BBR_RS16810 0.75 +2.0 1,541,397 + BBR_RS16810 0.75 +0.3 1,541,397 + BBR_RS16810 0.75 +1.6 1,541,402 + BBR_RS16810 0.75 +0.5 1,541,405 + BBR_RS16810 0.76 +1.1 1,541,405 - BBR_RS16810 0.76 +0.1 1,541,413 - BBR_RS16810 0.76 -1.4 1,541,435 + BBR_RS16810 0.79 -0.0 1,541,470 + BBR_RS16810 0.82 -0.6 1,541,516 - BBR_RS16810 0.87 -0.1 1,541,535 - BBR_RS16810 0.89 +1.1 1,541,545 - +0.0
Or see this region's nucleotide sequence