Experiment: CDM1 without Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15320 and BBR_RS15325 are separated by 216 nucleotides BBR_RS15325 and BBR_RS15330 are separated by 10 nucleotides BBR_RS15330 and BBR_RS15335 are separated by 24 nucleotides BBR_RS15335 and BBR_RS15340 are separated by 469 nucleotides
BBR_RS15320: BBR_RS15320 - hypothetical protein, at 1,211,353 to 1,211,622
_RS15320
BBR_RS15325: BBR_RS15325 - hypothetical protein, at 1,211,839 to 1,212,024
_RS15325
BBR_RS15330: BBR_RS15330 - hypothetical protein, at 1,212,035 to 1,212,556
_RS15330
BBR_RS15335: BBR_RS15335 - hypothetical protein, at 1,212,581 to 1,213,315
_RS15335
BBR_RS15340: BBR_RS15340 - hypothetical protein, at 1,213,785 to 1,214,480
_RS15340
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1211.613 kb on + strand at 1211.632 kb on + strand at 1211.667 kb on + strand at 1211.777 kb on - strand at 1211.845 kb on - strand at 1211.845 kb on - strand at 1211.890 kb on + strand, within BBR_RS15325 at 1211.912 kb on - strand, within BBR_RS15325 at 1211.943 kb on + strand, within BBR_RS15325 at 1211.943 kb on + strand, within BBR_RS15325 at 1211.965 kb on - strand, within BBR_RS15325 at 1211.983 kb on - strand, within BBR_RS15325 at 1211.983 kb on - strand, within BBR_RS15325 at 1211.996 kb on - strand, within BBR_RS15325 at 1212.041 kb on + strand at 1212.079 kb on + strand at 1212.079 kb on + strand at 1212.079 kb on + strand at 1212.180 kb on - strand, within BBR_RS15330 at 1212.180 kb on - strand, within BBR_RS15330 at 1212.226 kb on - strand, within BBR_RS15330 at 1212.311 kb on + strand, within BBR_RS15330 at 1212.338 kb on - strand, within BBR_RS15330 at 1212.346 kb on - strand, within BBR_RS15330 at 1212.553 kb on + strand at 1212.554 kb on + strand at 1212.562 kb on - strand at 1212.697 kb on + strand, within BBR_RS15335 at 1212.706 kb on + strand, within BBR_RS15335 at 1212.793 kb on - strand, within BBR_RS15335 at 1212.836 kb on + strand, within BBR_RS15335 at 1212.844 kb on - strand, within BBR_RS15335 at 1213.044 kb on + strand, within BBR_RS15335 at 1213.098 kb on + strand, within BBR_RS15335 at 1213.118 kb on - strand, within BBR_RS15335 at 1213.149 kb on + strand, within BBR_RS15335 at 1213.154 kb on + strand, within BBR_RS15335 at 1213.159 kb on + strand, within BBR_RS15335 at 1213.159 kb on + strand, within BBR_RS15335 at 1213.228 kb on + strand, within BBR_RS15335 at 1213.245 kb on - strand at 1213.318 kb on - strand at 1213.371 kb on - strand at 1213.371 kb on - strand at 1213.378 kb on - strand at 1213.451 kb on + strand at 1213.451 kb on + strand at 1213.459 kb on - strand at 1213.459 kb on - strand at 1213.459 kb on - strand at 1213.459 kb on - strand at 1213.487 kb on - strand at 1213.557 kb on + strand at 1213.651 kb on - strand at 1213.684 kb on - strand at 1213.684 kb on - strand at 1213.790 kb on + strand at 1213.833 kb on + strand at 1213.876 kb on + strand, within BBR_RS15340 at 1214.006 kb on - strand, within BBR_RS15340 at 1214.049 kb on + strand, within BBR_RS15340 at 1214.061 kb on + strand, within BBR_RS15340 at 1214.192 kb on + strand, within BBR_RS15340 at 1214.233 kb on + strand, within BBR_RS15340 at 1214.260 kb on + strand, within BBR_RS15340 at 1214.298 kb on - strand, within BBR_RS15340
Per-strain Table
Position Strand Gene LocusTag Fraction CDM1 without Threonine remove 1,211,613 + -2.2 1,211,632 + -1.5 1,211,667 + -2.5 1,211,777 - +0.3 1,211,845 - -2.5 1,211,845 - -2.2 1,211,890 + BBR_RS15325 0.27 +0.2 1,211,912 - BBR_RS15325 0.39 -2.3 1,211,943 + BBR_RS15325 0.56 -0.8 1,211,943 + BBR_RS15325 0.56 -1.6 1,211,965 - BBR_RS15325 0.68 -2.1 1,211,983 - BBR_RS15325 0.77 -2.8 1,211,983 - BBR_RS15325 0.77 -0.2 1,211,996 - BBR_RS15325 0.84 -0.8 1,212,041 + +2.2 1,212,079 + -1.6 1,212,079 + +1.1 1,212,079 + -1.6 1,212,180 - BBR_RS15330 0.28 +1.8 1,212,180 - BBR_RS15330 0.28 -0.8 1,212,226 - BBR_RS15330 0.37 -0.5 1,212,311 + BBR_RS15330 0.53 -1.4 1,212,338 - BBR_RS15330 0.58 -1.8 1,212,346 - BBR_RS15330 0.60 -2.4 1,212,553 + -0.8 1,212,554 + -1.4 1,212,562 - -1.5 1,212,697 + BBR_RS15335 0.16 -0.9 1,212,706 + BBR_RS15335 0.17 -2.1 1,212,793 - BBR_RS15335 0.29 -2.6 1,212,836 + BBR_RS15335 0.35 -0.2 1,212,844 - BBR_RS15335 0.36 -3.1 1,213,044 + BBR_RS15335 0.63 -2.8 1,213,098 + BBR_RS15335 0.70 +0.5 1,213,118 - BBR_RS15335 0.73 -1.2 1,213,149 + BBR_RS15335 0.77 -1.6 1,213,154 + BBR_RS15335 0.78 -1.5 1,213,159 + BBR_RS15335 0.79 -1.4 1,213,159 + BBR_RS15335 0.79 -0.9 1,213,228 + BBR_RS15335 0.88 -3.0 1,213,245 - -1.1 1,213,318 - +0.3 1,213,371 - +0.0 1,213,371 - -1.3 1,213,378 - +0.2 1,213,451 + -2.7 1,213,451 + -1.9 1,213,459 - -1.8 1,213,459 - -1.1 1,213,459 - +0.1 1,213,459 - +0.8 1,213,487 - -0.1 1,213,557 + -1.0 1,213,651 - +0.2 1,213,684 - +0.1 1,213,684 - -1.0 1,213,790 + -2.2 1,213,833 + -2.8 1,213,876 + BBR_RS15340 0.13 -1.6 1,214,006 - BBR_RS15340 0.32 -0.7 1,214,049 + BBR_RS15340 0.38 -1.4 1,214,061 + BBR_RS15340 0.40 -2.2 1,214,192 + BBR_RS15340 0.58 +0.2 1,214,233 + BBR_RS15340 0.64 +0.8 1,214,260 + BBR_RS15340 0.68 -0.5 1,214,298 - BBR_RS15340 0.74 +1.1
Or see this region's nucleotide sequence